Preparation method of bovine serum albumin-fenoxycarb nanodrug used for preventing and treating eucryptorrhynchus chinenis

Abstract

The invention discloses a preparation method of a bovine serum albumin-fenoxycarb nanodrug used for preventing and treating eucryptorrhynchus chinenis. A physical embedding method is adopted for the process of wrapping fenoxycarb with bovine serum albumin for preventing and treating the eucryptorrhynchus chinenis. The preparation method comprises the following steps: firstly taking a certain amount of bovine serum albumin (BSA) and dissolving in double-distilled water to be prepared into an aqueous phase, and taking a certain amount of fenoxycarb and dissolving in acetonitrile to be prepared into an oil phase; then under the condition of stirring at high speed, dropwise adding the oil phase into the aqueous phase, emulsifying for a period of time, then rapidly cooling, carrying out ultrasonic disintegration to obtain ultramicro emulsion, and cooling; and finally, carrying out cold drying, so that fenoxycarb albumin nanoparticles are obtained. The preparation method adopts a novel albumin nanoparticle preparation technology that drug is wrapped in albumin without changing the structure of albumin, drug loading capacity is increased, and slow release effect is realized. Usage amount, residual amount, cost and environmental pollution of chemical pesticide can be reduced by utilizing a nanotechnology; meanwhile, pesticide effect can be improved, contact area between a medicament and a target pest is effectively increased, and high efficiency, low toxicity and safety of the chemical pesticide are really realized.

Description

technical field [0001] The invention discloses a preparation method of bovine serum albumin-fenoxycarb nano-medicine for preventing and treating groove-orbital phenomenon, and the preparation process of fenoxycarb nano-particles belongs to the technical fields of life science, chemistry, nano-materials and new pesticides. Background technique [0002] Fenoxycarb, English name Ethyl 2-(4-phenoxyphenoxy)ethylcarbamate , the molecular formula is C 17 h 19 NO 4 , fenoxycarb, fenoxycarb or fenoxycarb, benzene is a non-terpene insect growth regulator insecticide with juvenile hormone activity, which was first put on the market by the Swiss company Syngenta in the 1990s , and was listed by Syngenta as one of the dozen or so reserved varieties in the 21st century. The pure product of fenoxycarb is a colorless crystal, melting point 53-54 ℃, solubility (at 25 ℃): 5.7mg / Kg in water, more than 250g in acetone, chloroform, ethyl ether acetate, methanol, isopropanol, toluene, etc. / ...

AI Technical Summary

Problems solved by technology

Use the embedding technology to make pure drug nanoparticles into nano-biological pesticide microspheres, and play the unique volume effect, surface effect, quantum size effect and macro-quantum tunnel effect of nanomaterials, and significantly improve the efficacy of pesticides. Traditional pesticide application methods It is through the spraying method, through which 80-90% of the active ingredients of the drug will be lost to the soil and the air, directly resulting in an increase in the amount of pesticide application and the increase in the frequency of application, etc.

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