Extraction purification and detection method of basella alba volatile oil and application of basella alba volatile oil

A purification method and volatile oil technology are applied in the field of research and development of pharmaceutical products, can solve problems such as reporting of volatile oil of tofu and vegetables, and achieve the effects of low cost, good anti-oxidation and simple process

Active Publication Date: 2017-06-20
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet there is not yet the report about the volatile oil of bean curd dish in the prior art

Method used

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  • Extraction purification and detection method of basella alba volatile oil and application of basella alba volatile oil
  • Extraction purification and detection method of basella alba volatile oil and application of basella alba volatile oil
  • Extraction purification and detection method of basella alba volatile oil and application of basella alba volatile oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 The extraction and purification method of bean curd vegetable volatile oil

[0041] (1) Take 500g of fresh bean curd, wash and dry it, then grind it to a particle size of 60 mesh, add 1000g of distilled water, stir and carry out steam distillation, and the distillation extraction time is 5 hours to obtain a water system containing volatile oil;

[0042] (2) Add ether to the water system containing volatile oil for extraction, discard the separated water layer, add anhydrous sodium sulfate to the ether solution containing volatile oil for dehydration, filter and recover ether to obtain dry and pure volatile oil.

Embodiment 2

[0043] Example 2 Detection method of bean curd vegetable volatile oil

[0044] Adopt GC-MS to detect the bean curd volatile oil that embodiment 1 obtains, and concrete detection condition is as follows: HP-5MS chromatographic column (30m * 0.25mm * 0.25 μ m); Carrier gas: helium; Flow rate: 1.2mL / min, solvent Delay for 4 minutes; heating program: the initial temperature is 60°C, hold for 1 minute, raise the temperature to 200°C at 15°C / min, hold for 5 minutes, then rise to 280°C at 5°C / min, and hold for 2 minutes. Ionization method: EI, 70eV; ion source temperature: 250°C; carrier gas: helium; column flow rate: 1.2mL / min. A total of 49 chemical components in the volatile oil of tofu were detected, and the specific chemical components and relative contents are shown in Table 1.

[0045] Table 1 Chemical composition of volatile oil in bean curd

[0046]

[0047]

Embodiment 3

[0048] Example 3 Antioxidation test of volatile oil of bean curd

[0049] 1 Preparation of DPPH test solution Preparation of 0.08mmol / L DPPH solution: Accurately weigh 8.0mg of DPPH, dissolve it with absolute ethanol and set the volume to a 200ml brown volumetric flask to obtain a DPPH solution with a concentration of 0.004%, and store it away from light. spare.

[0050] 2 Experimental steps: Take 1.0ml of each sample solution (0.24, 0.48, 0.72, 0.96, 1.20mg / ml) of different concentrations, put it into a 10ml centrifuge tube, add 3.0ml of DPPH solution, react at room temperature for 30min in the dark, and at the same time Absorbance was measured at a wavelength of 517 nm with absolute ethanol as a blank. Calculate the DPPH free radical scavenging rate according to the following formula. DPPH free radical scavenging rate (%)=A0-(As-Ac) / A0×100% In the formula, A0—absorbance value of 1.0ml distilled water + 3.0ml DPPH solution As—absorbance value of 1.0ml sample solution + 3.0m...

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Abstract

The invention discloses an extraction purification and detection method of basella alba volatile oil and application of the basella alba volatile oil. A vapor distillation and organic extraction technology is firstly adopted to extract and purify the basella alba volatile oil, the extraction and purification method is simple in process and low in cost, distilled water and organic solvent in the preparation process are reusable, and the volatile oil obtained by the preparation process of the invention is low in water content, is proved by virtue of test to have good antioxidant, antibacterial and anticancer activity at the same time, has a good bacteriostatic effect on staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, has a good anticancer and cancer inhibition effect on HepG2 (human liver cancer cells) and MCF-7 (human breast cancer cells) and is suitable for the industrialized production and application.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products, and in particular relates to a method for extracting, purifying and detecting the volatile oil of bean curd and vegetable and its application. Background technique [0002] Tofu dish (Premna microphylla Turcz), belongs to Verbenaceae, Tofuchai plants, aliases are stinky yellow vitex, tofu wood, tofuchai, tofu leaf, Guanyinchai and jelly chai, the roots, stems and leaves are used as medicine, can clear away heat and detoxify, Reduce swelling and stop bleeding. Indications for poisonous snake bites, unknown swelling, bleeding from trauma, etc. It is widely distributed in my country, mainly in the south of the Yangtze River Basin, including East China, Central South, South China, Sichuan, Guizhou and other places. It is suitable for growing on hillsides, forest margins, under sparse forests, and shrubs on both sides of streams below 1000m above sea level....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02G01N30/02A61K36/85A61P39/06A61P31/04A61P35/00
CPCA61K36/85C11B9/022C11B9/025C11B9/027G01N30/02Y02A50/30
Inventor 赖鹏翔高阳张瀚予
Owner SHANDONG UNIV
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