Improved rhizoma bletillae tissue culture seedling production method
A production method and technology of tissue culture seedlings, applied in the field of producing high-quality white and tissue culture seedlings, can solve the problems of thin seedlings, small diameter of pseudobulbs, long tissue culture time, etc.
Inactive Publication Date: 2017-06-23
云南天泉生物科技股份有限公司
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Problems solved by technology
At present, there are also tissue culture rooms in China to carry out the production of white and tissue culture seedlings, but the common phenomenon is that the seedlings are thinner, the diameter of the pseudobulb formed is smaller, the survival rate of tissue culture seedlings is not high, and the tissue culture time is longer
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Embodiment 1
[0032] Embodiment 1 The inventive method comprises the following steps:
[0033] (1) Sterilization of white and capsules and sowing and cultivation in bottles
[0034] Wipe the surface of the mature white and capsule with alcohol with a volume fraction of 75%. The mature white and capsule are the fruits whose growth cycle reaches 22 weeks, and then use the washing powder water with a mass fraction of 3% to clean the mature. The surface of the pods and pods was then placed on a clean bench and sterilized with a 0.2% mercuric chloride solution for 15 minutes, then rinsed with sterile water for 3 to 5 times, and the moisture on the pods of pods was blotted dry with sterilized filter paper. Use a scalpel to cut off the top of the Baiji capsule, then cut it longitudinally from the middle of the Baiji capsule, take out the seeds and spread them evenly in bottles containing the seed sowing medium, and use thin sowing, the sowing amount is 60 bottles of Baiji capsule. , the bottle is...
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The invention discloses an improved rhizoma bletillae tissue culture seedling production method. The method is characterized in that the common culture medium MS serves as control, the use amount of phosphorus and potassium in macroelements is increased, and the use amount of nitrogen, calcium and magnesium is reduced. The use amount of B vitamins is increased, choline chloride, AgNO3, citric acid and compound sodium nitrophenolate are newly increased, and low-concentration plant hormone 6-BA of 0.02 to 0.2 mg / L and NAA of 0.02 to 0.5 mg / L are adopted. The temperature of a culture room is increased to 28 DEG C, the illumination intensity of a sowing stage, a strong seedling stage and a rooting stage is adjusted, and rhizoma bletillae seedlings are produced by a four-step method of seed sowing, strong seedling cultivation, rooting cultivation and seedling shining cultivation. By the method provided by the invention, the quality of the rhizoma bletillae tissue culture seedlings is improved remarkably, the production cycle is shortened to be 170 to 180 days, the cultivation cycle is shortened, the rhizoma bletillae tissue culture seedlings grow strongly, the formed pseudobulb reaches above 0.8 cm, and the bottle-out transplanting survival rate reaches above 90 percent.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for producing high-quality bletilla root tissue culture seedlings by improving key components in a bletilla root tissue culture medium. Background technique [0002] The orchid plant Bletilla striata (Thunb.) Reichb.f.) was included in the Chinese Pharmacopoeia (2000 edition 1), and its functions and indications are astringent to stop bleeding, detumescence and granulation. For hemoptysis and hematemesis, traumatic hemorrhage, sore swelling, chapped skin; pulmonary tuberculosis hemoptysis, ulcer disease hemorrhage. [0003] In recent years, it has been found that Baiji polysaccharide gum has a special viscosity and can be used as a suspending agent and emulsifier in the food and chemical fields. Modern pharmacological studies have shown that Baiji polysaccharide gum has inhibitory effects on Staphylococcus, Streptococcus, Mycobacterium tuberculo...
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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张国武万春雷周正娇吴旻潘艳玲李琼华
Owner 云南天泉生物科技股份有限公司