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Modification method for dietary fiber of black fungus

A technology of dietary fiber and black fungus, applied in the functions of food ingredients, food extraction, food science and other directions, can solve the problems of high viscosity, unfavorable enzyme molecular fluidity and diffusion, increased difficulty in the extraction and preparation process, and increased viscosity of the extraction system, etc. To achieve the effect of loose macromolecule structure of dietary fiber, good sensory properties, and improvement of cell wall

Active Publication Date: 2017-06-30
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, black fungus is a special colloidal fungus, which is different from common edible fungi (such as: mushrooms), and its carbohydrate content is very high. After the black fungus absorbs water and swells, the soluble part from the tissue The outflow increases the viscosity of the extraction system and increases the difficulty of the extraction and preparation process of black fungus polysaccharides, especially for the preparation method that needs to use enzyme catalysis in the preparation process. High viscosity is not conducive to the fluidity and diffusion of enzyme molecules

Method used

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  • Modification method for dietary fiber of black fungus
  • Modification method for dietary fiber of black fungus
  • Modification method for dietary fiber of black fungus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1 Materials and reagents

[0033] Black fungus (provided by Hangzhou Huadan Agricultural Products Co., Ltd.). Before use, dry in an oven at 50°C, pulverize, and sieve samples with a mesh size of 40-60 mesh for later use.

[0034] Phenol, concentrated sulfuric acid, DPPH, absolute ethanol are analytically pure;

[0035] Cellulase (activity 10000u / g) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0036] 2 experimental equipment

[0037] Grinder, AL04 electronic balance, HH-2 water bath, drying oven, centrifuge, agitator, spectrophotometer, ultrasonic breaker

[0038] 3 Experimental methods

[0039] 3.1 Preparation of black fungus DF

[0040] Crush black fungus, pass through a 40-60 mesh sieve, take 40g of black fungus coarse powder, mix with 2000g of deionized water, stir and extract at 80°C for 4 hours, centrifuge for 30min (4000r / min), discard the supernatant, and The slag was washed repeatedly with deionized water, vacuum-dried, pulverized...

Embodiment 2

[0111] The experimental operation is the same as in Example 1, the difference is that 20g of black fungus DF is taken, deionized water is added according to the ratio of material to liquid at 1:50, and then 120ml of cellulase (0.9%) is added (the mass ratio of black fungus DF is 5.4 %), put it into an ultrasonic breaker, and ultrasonicate it for 10 minutes under the conditions of ultrasonic frequency 30kHz, power 4W, and treatment temperature 50°C, then take it out and put it in a water bath to continue enzymatic hydrolysis for 260 minutes, and then make up the ratio of solid to liquid to 1: 110, put it into the ultrasonic breaker again, and ultrasonicate for 20 minutes under the condition of ultrasonic frequency 30kHz and power 4W. The average value of total sugar content is 12.35%, the scavenging rate for DPPH·91.21%, the scavenging rate for hydroxyl radicals is 68.41%, and the binding water capacity is 22.84g / g.

Embodiment 3

[0113] The experimental operation is the same as in Example 1, except that 20g of black fungus DF is taken, deionized water is added according to the ratio of material to liquid at 1:50, and then 130ml of cellulase (0.9%) is added (the mass ratio of black fungus DF is 5.85%) %), put it into an ultrasonic breaker, and ultrasonicate for 5 minutes at an ultrasonic frequency of 20kHz, a power of 4W, and a treatment temperature of 50°C, then take it out and put it in a water bath to continue enzymatic hydrolysis for 265 minutes, and then make up the ratio of solid to liquid to 1: 110, put it into the ultrasonic breaker again, and ultrasonicate for 25 minutes under the condition of ultrasonic frequency 20kHz and power 4W. The average value of total sugar content is 12.13%, the scavenging rate for DPPH·91.21%, the scavenging rate for hydroxyl radicals is 68.31%, and the binding water capacity is 24.35g / g.

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Abstract

The invention provides a modification method for dietary fiber of black fungus. The modification method comprises the following steps: taking dry black fungus, mechanically crushing, sieving, mixing obtained rough black fungus powder with deionized water, stirring, extracting, then carrying out centrifugation, removing supernatant, cleaning slags, carrying out vacuum drying, crushing, and thus obtaining the dietary fiber of the black fungus; mixing the dietary fiber of the black fungus with the deionized water, then adding cellulase, carrying out ultrasonic treatment for 5-20 minutes at the temperature of 40-60 DEG C, then maintaining the temperature to carry out enzymolysis for 240-280 minutes, then supplementing the deionized water, then carrying out ultrasonic treatment for 10-25 minutes, and then freezing and drying to obtain the modified dietary fiber of the black fungus. The modification method provided by the invention has the advantages that the black fungus is adopted as a raw material, the dietary fiber of the black fungus is modified by adopting a modifying treatment process with optimized ultrasound and conjugated enzyme under specific conditions, soluble sugar in the dietary fiber of the black fungus can be effectively dissolved out, and active groups are fully exposed, so that the characteristics such as hydratability and oxidation resistance can be effectively improved.

Description

[0001] (1) Technical field [0002] The invention relates to a method for modifying black fungus dietary fiber. [0003] (2) Background technology [0004] Dietary fiber (dietary fiber, DF) usually refers to the sum of edible plant ingredients, carbohydrates and similar substances that can resist digestion and absorption in the small intestine of the human body and can be partially or completely fermented in the large intestine of the human body, including some indigestible Polysaccharides, oligosaccharides, lignin and other plant associations. According to the solubility characteristics of DF, it can be divided into water-soluble dietary fiber (soluble dietary fiber, SDF) and water-insoluble dietary fiber (insoluble dietary fiber, IDF), SDF mainly includes dextran, resistant dextrin, carboxymethyl cellulose , plant colloid, etc., IDF mainly includes cellulose, hemicellulose, lignin, etc. Although DF cannot be digested and absorbed by the human body, a large number of studies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/21A23L5/30A23L29/00A23L31/00
CPCA23V2002/00A23V2200/30A23V2300/48A23V2250/208A23V2300/14A23V2250/5116
Inventor 张拥军卢傲雪张雪绒宗丽菁姚晓瞳
Owner CHINA JILIANG UNIV
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