A functionalized temperature-sensitive polymer, a preparing method thereof and applications of the polymer

A temperature-sensitive polymer technology, applied in the field of analytical chemistry, can solve the problems of reducing the enrichment reaction rate and efficiency, and achieve the reduction of solid-liquid interface mass transfer resistance and steric hindrance, high enrichment efficiency and selectivity, Reactive Bioorthogonal Effects
CN106905472AActive Publication Date: 2017-06-30ACAD OF MILITARY SCI ACAD OF MILITARY MEDICAL SCI BEIJING INST OF LIFEOMICS
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ACAD OF MILITARY SCI ACAD OF MILITARY MEDICAL SCI BEIJING INST OF LIFEOMICS
Publication Date
2017-06-30

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Abstract

A functionalized temperature-sensitive polymer, a preparing method thereof and applications of the polymer is disclosed. The structure formula of the polymer is shown as a formula I. The preparing method includes subjecting a temperature-sensitive monomer and a functional monomer to free radical polymerization under the existence of an initiator to obtain a temperature-sensitive polymer; subjecting the temperature-sensitive polymer and hydrazine hydrate to an acylhydrazine conversion reaction to obtain a hydrazide-modified temperature-sensitive polymer; and reacting the hydrazide-modified temperature-sensitive polymer with 2-(diphenylphosphino) terephthalic acid 1-methyl 4-pentafluorophenyl diester. An azide-marked O-G1cNAc modified protein enriching mechanism of the functionalized temperature-sensitive polymer is based on a Staudinger ligation reaction between azide and triphenylphosphine, and the reaction is high in biological orthogonality, high in yield in an aqueous solution, specific and stable and does not need a catalyst, and therefore the reaction has good compatibility with subsequent mass spectrum identification. The method greatly increases the amino acid sequence coverage rate of low-abundance O-G1cNAc modified protein mass spectrum identification in a mixed sample.
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Description

technical field

[0001] The invention relates to a functionalized temperature-sensitive polymer and its preparation method and application, belonging to the field of analytical chemistry. Background technique

[0002] O-linked β-N-acetylglucosamine (O-GlcNAc) is a post-translational modification ubiquitous in eukaryotic cells. Different from the classic O-glycosylation modification, O-GlcNAc glycosylation modification only exists in nuclear and cytoplasmic proteins, has only one monosaccharide structure, and is a highly dynamic, inducible and reversible modification method . Although the abundance of this modification on proteins is extremely low, O-GlcNAc modified proteins play an important role in organisms, from gene transcription to signal transduction and cell cycle regulation, there are O-GlcNAc glycosylated modified proteins participate. Studies have shown that abnormal levels of O-GlcNAc glycosylation modification on proteins are closely related to many human disea...

Claims

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