PML/RARA (promyelocytic leukemia/retinoic acid receptor alpha) fusion gene quick detection probe with low cost and preparation method and application thereof

A technology of fusion genes and detection probes, applied in the field of molecular biology, can solve the problems of increased preparation costs and uncontrollable factors, increased probe preparation costs, instability between batches, etc., to achieve high specificity and sample detection High efficiency, reduced preparation cost, and high labeling efficiency
CN106929576AActive Publication Date: 2017-07-07WUHAN HEALTHCHART BIOLOGICAL TECH
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
WUHAN HEALTHCHART BIOLOGICAL TECH
Publication Date
2017-07-07

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Abstract

The invention belongs to the field of molecule biology, and particularly relates to a PML / RARA (promyelocytic leukemia / retinoic acid receptor alpha) fusion gene quick detection probe with low cost and a preparation method thereof. The preparation method comprises the following steps of downloading non-repeated sequences of g genomeene groups in the area covered by PML and RARA gene probes from USCS Genome Browser, and importing the segmented sequences into OligoArray software by batches to design the probes; adding a tag sequence onto the designed probe, and directly synthesizing; using a tag primer with a fluorophorefluorescent gene to amplify and mark the probe, so as to obtain the PML / RARA fusion gene quick detection probe. The prepared PML / RARA fusion gene quick detection probe has the advantages that the hybridizing time is short, the hybridizing experiment can be completed within 15 to 30min, the hybridizing signal is bright, the signal to noise ratio is high, the preparation cost is low, and the like.
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Description

technical field

[0001] The invention belongs to the field of molecular biology, and in particular relates to a low-cost rapid detection probe of PML / RARA fusion gene and its preparation method and application. Background technique

[0002] Detection of PML / RARA fusion gene is one of the most specific and sensitive methods for diagnosing acute promyelocytic leukemia (APL), and it is also the most reliable indicator for APL treatment options, curative effect analysis and prognosis analysis. At present, karyotype analysis, PCR method and FISH method are several common detection methods. FISH has the advantages of safety, rapidity, high sensitivity and simultaneous display of multiple colors.

[0003] Fluorescence in situ hybridization is a non-radioactive molecular genetic technique developed on the basis of radioactive in situ hybridization in the 1980s. It is a new in situ hybridization method formed by replacing radioactive isotope labels with fluorescein labels. At presen...

Claims

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