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Method for preparing anti-pollution surface of prism type surface plasmon resonance chip based on modification of hyaluronic acid coupled lubricin proteins

A technology of surface plasma and hyaluronic acid, which is applied in measurement devices, material analysis by optical means, instruments, etc., can solve the problems of surface contamination of sensor chips, false positives, and reduce the accuracy of quantitative detection, and achieve anti-pollution performance. No change, strong anti-pollution performance, simple and fast preparation method

Inactive Publication Date: 2017-07-07
TIANJIN UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when surface plasmon resonance is used for analysis and testing, the surface contamination of the sensor chip is a common problem, and non-specific adsorption from biomolecules or microorganisms in the sample will reduce the accuracy of quantitative detection and produce false positive results

Method used

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  • Method for preparing anti-pollution surface of prism type surface plasmon resonance chip based on modification of hyaluronic acid coupled lubricin proteins
  • Method for preparing anti-pollution surface of prism type surface plasmon resonance chip based on modification of hyaluronic acid coupled lubricin proteins

Examples

Experimental program
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Effect test

Embodiment 1

[0027] A 2nm-thick chromium layer was coated on the BK7 glass substrate by electron beam evaporation coating technology, and a 48nm-thick gold film was coated on the chromium layer to obtain a bare gold chip; the obtained bare gold chip was put into ultraviolet light Clean in an ozone cleaner for 20 minutes, take it out, wash it with ethanol and deionized water three times in sequence, and then blow dry with nitrogen; use degassed pure water to prepare a 1 mg / mL hyaluronic acid (HA) solution containing mercaptolation, and dry it After the chip was attached to the prism of the surface plasmon resonance system with asphalt, the phosphate buffer solution with a pH of 7.4 was used as the mobile phase, and the flow rate was 50 μL / min to continuously flow over the chip surface. When the baseline was stable, 100 μL of 1 mg / mL thiolated hyaluronic acid solution, and flowed through the flow cell at a rate of 20 μL / min. When the signal became stable, 100 μL of the above thiolated hyaluro...

Embodiment 2

[0029]A 2nm-thick chromium layer was coated on the BK7 glass substrate by electron beam evaporation coating technology, and a 47nm-thick gold film was coated on the chromium layer to obtain a bare gold chip; the obtained bare gold chip was put into ultraviolet light Clean in an ozone cleaner for 20 minutes, take it out, wash it with ethanol and deionized water three times in turn, and then blow dry with nitrogen; prepare a 2 mg / mL hyaluronic acid (HA) solution containing sulfhydrylation with degassed pure water, and dry it After the chip was attached to the prism of the surface plasmon resonance system with asphalt, the phosphate buffer solution with a pH of 7.4 was used as the mobile phase, and the flow rate was 100 μL / min to continuously flow over the surface of the chip. When the baseline was stable, inject 100 μL of 2 mg / mL thiolated hyaluronic acid solution, and flowed through the flow cell at a rate of 10 μL / min. When the signal became stable, inject 100 μL of the above t...

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Abstract

The invention discloses a method for preparing an anti-pollution surface of a prism type surface plasmon resonance chip based on modification of hyaluronic acid coupled lubricin proteins. The method comprises the steps of linking hyaluronic acid with a terminal end containing dopamine groups to a surface of a metal film through polymerization of dopamine, and winding hyaluronic acid with lubricin proteins by virtue of physical effects such as static electricity so as to form a self-assembled monomolecular layer, namely the prism type surface plasmon resonance chip based on the hyaluronic acid coupled lubricin proteins. The chip prepared by virtue of the method has relatively strong anti-pollution performance, good lubricating property and biological compatibility and high stability, and the anti-pollution performance is not changed after the chip is stored at a drying state for three months or stored at room temperature for four months; and the method for preparing the chip is relatively simple, convenient and rapid, a multistep complicated preparation process in the past is reduced, and the surface modification efficiency of the chip is improved.

Description

technical field [0001] The invention relates to the field of preparation of an anti-pollution chip for a surface plasmon resonance spectrometer, in particular to a method for preparing a surface plasmon resonance chip based on hyaluronic acid-coupled lubricating protein modification. Background technique [0002] The Surface Plasmon Resonance (SPR) instrument is a biosensor technology that appeared in the 1980s. When the incident light is incident from a high-refractive-index medium to a low-refractive-index medium, total reflection will occur. A layer of metal film (gold or silver) is coated at the interface of the medium. The evanescent wave generated by the incident light will cause the free electrons on the metal surface to oscillate collectively, and then form plasma. When the frequency and wave number of the evanescent wave and surface plasmon oscillation When they are equal, the resonance phenomenon (SPR) will occur, which will cause the energy to transfer from the ev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552
CPCG01N21/554
Inventor 苏荣欣叶慧君黄仁亮齐崴
Owner TIANJIN UNIV
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