Pleurotus primary culture compound culture medium and preparation method thereof

A culture medium and a technology of Pleurotus genus are applied in the field of a first-class strain compound medium of Pleurotus genus and the field of preparation thereof, which can solve the problems of waste of resources of S. The effect of chemical industry production, strong mycelium and short growth cycle

Active Publication Date: 2017-07-14
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems existing in the prior art, the present invention provides a compound culture medium of the first-class strain of Pleurotus and its preparation method. The culture medium utilizes Sargassum polysaccharide as a supplementary carbon source, effectively solving the problem of my country's coastal Sargassum The problem of

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  • Pleurotus primary culture compound culture medium and preparation method thereof
  • Pleurotus primary culture compound culture medium and preparation method thereof
  • Pleurotus primary culture compound culture medium and preparation method thereof

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preparation example Construction

[0033] The preparation method of the sage polysaccharide crude extract of the present invention can refer to the preparation method of the prior art, such as microwave extraction, ultrasonic extraction or ultrasonic enzymatic extraction, the sage polysaccharide crude extract in the present embodiment 1-4 The preparation method is obtained by referring to the optimal technical scheme in the process research of Li Qingtao et al. for extracting sargassum polysaccharides by microwave method. The specific process is: microwave time 8min, microwave power 540W, solid-liquid ratio 1:30, ultrasonic time 3min, the obtained crude The polysaccharide content is 26.7%.

[0034] Preparation of 1L plate medium A: Peel the potatoes, dig out the teeth, wash and weigh 150g, slice and boil with water until the potato slices are cooked and not rotten, filter with four layers of gauze to obtain the filtrate . Weigh peptone 1.5g, VB 6 0.015mg, potassium dihydrogen phosphate 2.0g, magnesium sulfat...

Embodiment 1

[0048] Inoculate 5 tubes of Pleurotus ostreatus strains stored in the refrigerator on the slant activated mother culture medium under aseptic conditions, and culture in a constant humidity incubator at 25° C. and a relative humidity of 50% for 5 days in shading. After 5 days, select the activated strain with the best growth status and inoculate it in the center of the PDA plate under aseptic conditions to ensure that the colony edge is continuous and the mycelium grows evenly, so that the growth of the mycelium used in the next punching inoculation is the same or similar. Five small plates (small Petri dishes) were inoculated for each strain. Humidity incubator 25 ℃, relative humidity 50%, shading culture 8d.

[0049] Select the plate with the best growth status after 8 days of cultivation, under sterile conditions, on the culture medium with mycelium growing at the same distance from the center of the plate, use a puncher to punch into uniform small pieces, directly 5 mm in s...

Embodiment 2

[0053] Inoculate 5 tubes of Pleurotus citrinopileatus strains stored in the refrigerator on the slant activated mother seed medium under aseptic adjustment, and inoculate 5 tubes in a constant humidity incubator at 25° C., with a relative humidity of 50%, and culture in shading for 5 days. After 5 days, select the activated strain with the best growth status and inoculate it in the center of the PDA plate under aseptic conditions to ensure that the colony edge is continuous and the mycelium grows evenly, so that the growth of the mycelium used in the next punching inoculation is the same or similar. Five small plates (small Petri dishes) were inoculated for each strain. Humidity incubator 25 ℃, relative humidity 50%, shading culture 8d.

[0054] Select the plate with the best growth status after 8 days of cultivation, under sterile conditions, on the culture medium with mycelium growing at the same distance from the center of the plate, use a puncher to punch into uniform smal...

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Abstract

The invention relates to a pleurotus primary culture compound culture medium which contains potatoes, a supplementing carbon source, a supplementing nitrogen source, a vitamin and inorganic salts. The supplementing carbon source is polysaccharide of salvia, the supplementing nitrogen source is one of yeast powder, beef extract or peptone, the vitamin is VB6, and the inorganic salt contains potassium dihydrogen phosphate, magnesium sulfate and calcium chloride. By taking extract of polysaccharide of salvia as a delayed carbon source, a primary culture of the pleurotus edible mushroom is exuberant in growth, the mycelia are dense and thick and strong, uniform in growing, more in germinating point and short in growing period, so that the vitality of the culture is effectively guaranteed; growth on a secondary high-salt culture medium is facilitated, and a lot of manpower and material resources are saved for industrially artificial cultivation. According to the technical scheme provided by the invention, recycling of a sargassum thunbergii resource is facilitated, the cost is low, and industrial production on a large scale is facilitated.

Description

technical field [0001] The invention relates to a culture medium for strains, in particular to a compound culture medium for first-grade strains of the genus Pleurotus and a preparation method thereof. Background technique [0002] Fungi of the genus Pleurotus are rich in nutrients, including polysaccharides, proteins, amino acids, fatty acids, etc. These bioactive ingredients have important functions such as improving immunity, delaying aging, anti-fatigue, anti-tumor, regulating blood sugar, regulating blood lipids, and chemoprevention. The fungi of the genus Pleurotus have pharmacological activity and have health-care effects on the human body. They are a kind of good edible fungi with great medicinal potential and edible value. The genus Pleurotus mainly includes Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus ostreatus, Pleurotus ostreatus, etc. Among them, Pleurotus ostreatus and Pleurotus ostreatus are important medicinal fungi. At present, there are few artificia...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 张志健
Owner ZHEJIANG OCEAN UNIV
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