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Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose contents

A technology of xylo-oligosaccharides and xylotriose, applied in the direction of fermentation, etc., can solve the problems of low reaction intensity and low biological activity of xylo-oligosaccharides, and achieve the effects of high biological activity, low production cost, and small waste water pollution load.

Inactive Publication Date: 2017-07-28
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the xylan degradation reaction in the hydrothermal treatment is in a weakly acidic environment, the reaction intensity is low, so the xylooligosaccharides produced have fewer xylodisaccharides and xylotriose components with a low degree of polymerization, resulting in hydrothermal Xylo-oligosaccharides prepared by the method have low biological activity

Method used

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  • Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose contents
  • Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose contents
  • Preparation method of xylo-oligosaccharide with high xylobiose and xylotriose contents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 The hot water treatment of crop stalks

[0030] (1) Hot water treatment of wheat straw: mix 50g of dry wheat straw with 500mL of distilled water, cook at 180°C for 40min, after cooking, separate the solid and liquid with a cloth bag, and obtain two parts of hot water treated solid slag and hot water treated liquid.

[0031] (2) Analysis of the concentration of xylooligosaccharide components with different degrees of polymerization in the hot water treatment liquid and the proportion of the total xylooligosaccharides, the results are shown in Table 1.

[0032] Table 1 Concentrations of xylooligosaccharide components with different degrees of polymerization in hot water treatment liquid and their proportions in total xylooligosaccharides

[0033]

[0034] The results showed that when wheat straw was treated in hot water at 180°C for 40 minutes at a solid-to-liquid ratio of 1:10, most of the xylan in wheat straw was dissolved in the form of xylo-oligosaccha...

Embodiment 2

[0035] Example 2 Preparation of xylooligosaccharides with high xylobiose and xylotriose content by enzymatic hydrolysis of wheat straw hot water treatment liquid

[0036] Get 20 mL of the hot water treatment solution of Example 1 in a 150 mL hydrolysis bottle, adjust the pH value to 4.8 with 50% NaOH, add endoxylanase according to the enzyme dosage of 75U / g xylo-oligosaccharide, place in 50 Enzymolysis was carried out in a shaker at ℃ for 24 hours, 1 mL was sampled at different reaction times, centrifuged at 10,000 for 10 minutes, and the supernatant was taken to analyze the concentration of xylooligosaccharide components with different degrees of polymerization in the enzymolysis solution and the proportion of xylooligosaccharides in the total xylooligosaccharides. The results are shown in Table 2.

[0037] Table 2 Concentration of xylooligosaccharide components with different degrees of polymerization in the enzymatic hydrolysis solution and their proportions in the total xy...

Embodiment 3

[0040] Example 3 Effect of Enzyme Dosage on the Content of Xylobiose and Xylotriose in Xylooligosaccharides Prepared by Enzymolysis of Wheat Straw Hot Water Treatment Solution

[0041] Take respectively 20 mL of the hot water treatment solution of Example 1 in a 150 mL hydrolysis bottle, adjust the pH value to 4.8 with 50% NaOH, and add endo-xylo-oligosaccharides at an enzyme dosage of 50, 75, and 100 U / g xylo-oligosaccharides respectively. Carbohydrase, placed in a shaker at 50°C for 24 hours, after the enzymolysis, the hydrolyzate was centrifuged at 10,000 for 10 minutes, and the supernatant was taken to analyze the sum of the concentration of xylobiose and xylotriose in the enzymolysis solution and the total concentration The ratio of xylan. The results are shown in Table 3.

[0042] Table 3 Effect of enzyme dosage on xylobiose and xylotriose content in xylooligosaccharides prepared by enzymatic hydrolysis of wheat straw hot water treatment solution

[0043]

[0044] T...

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Abstract

The invention discloses a preparation method of xylo-oligosaccharide with high xylobiose and xylotriose contents. The xylo-oligosaccharide is prepared by the aid of a coupling mode of hot-water treatment of crop straws and endo-xylanase degradation, and the weight sum of the xylobiose and the xylotriose is smaller than 60% of total weight of the xylo-oligosaccharide. Experiments confirm that by the method, the xylo-oligosaccharide can be acquired through enzymolysis of 24h, and the weight percentage of the xylobiose and the xylotriose to the xylo-oligosaccharide is larger than 60%. The method effectively solves the problem of low biological activity of xylo-oligosaccharide prepared by a hydrothermal method, overcomes the shortcomings that xylan is difficult to extract and high in purification cost, wastewater pollution load is large and the like in a conventional method and has the advantages that chemicals cannot be added, production cost is low, wastewater pollution load is small, the biological activity of the prepared xylo-oligosaccharide is high and the like, and the xylo-oligosaccharide has good industrial prospect and practicability.

Description

technical field [0001] The invention relates to the technical field of preparation of functional oligosaccharides, in particular to a method for preparing xylooligosaccharides with high xylobiose and xylotriose content. [0002] technical background [0003] Hemicellulose is a kind of high polysaccharide in lignocellulosic raw materials after the content of cellulose, among which xylan is the main component of crop straw hemicellulose. The industrial utilization of xylan mainly includes the production of xylose, xylitol, furfural and xylooligosaccharides, etc., and the degradation of xylan into xylose, which is then fermented by microorganisms to produce bioenergy or chemicals such as ethanol. Sugar is the xylan derivative with the highest added value. At present, there are mainly two methods for preparing xylo-oligosaccharides from crop straws with industrial application prospects: one is to use alkali to extract xylan from crop straws, and the xylan is hydrolyzed by endoxy...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P19/12C12P19/14
CPCC12P19/04C12P19/12C12P19/14C12P2201/00
Inventor 勇强黄晨武新星黄阳赖晨欢李鑫陈牧徐勇欧阳嘉余世袁
Owner NANJING FORESTRY UNIV