Stable HRP (horse radish peroxidase) chemiluminescence substrate solution

An enzymatic chemiluminescence and substrate liquid technology, applied in the field of immunoassay, can solve the problems of inaccurate results, poor stability and high cost, and achieve the effects of high luminescence intensity value, good stability and low cost
CN106990100AActive Publication Date: 2017-07-28武汉海吉力生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
武汉海吉力生物科技有限公司
Publication Date
2017-07-28

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Abstract

The invention discloses stable HRP (horse radish peroxidase) chemiluminescence substrate solution which comprises luminescence agent solution and oxidant solution. The comprises luminescence agent solution is prepared by specific proportion of Luminol or Luminol derivative or Luminol sodium salt, p-Iodophenol, phenolphthalein and buffer solution, the p-Iodophenol serves as reinforcing agents, the phenolphthalein serves as synergistic reinforcing agents, the oxidant solution is prepared by specific proportion of urea peroxide, ethylene diamine tetraacetic acid, tween-20 and buffer solution, and the luminescence agent solution and the oxidant solution are mixed before use of the substrate solution. The HRP chemiluminescence substrate solution is simple to prepare, the p-Iodophenol serves as the reinforcing agents, the phenolphthalein serves as the synergistic reinforcing agents, the tween-20 serves as stabilizing agents, the stability of the chemiluminescence substrate solution is further improved, and the HRP chemiluminescence substrate solution is stable, high in signal-to-noise ratio and sensitivity, long in platform phase and purple red.
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Description

technical field

[0001] The invention relates to the field of immunoanalysis, in particular to a chemiluminescence substrate solution using HRP as an enzymatic reaction detection system. Background technique

[0002] Chemiluminescence immunoassay (CLIA) is a new type of labeled immunoassay technology for the detection of trace antigens or antibodies established by combining luminescence analysis and immune response. It has both the specificity of immune response and the High sensitivity with chemiluminescence reaction. It overcomes the radioactive hazard and pollution problems caused by the use of radioactive isotopes in radioimmunoassay (RIA); it overcomes the shortcomings of complex instruments and large background interference in fluorescence immunoassay (FIA). With its unique advantages such as high sensitivity, low instrument price, easy to use, safety, and no radioactive pollution, it is favored by people and has become an important direction of marker immunization. A...

Claims

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