Micro-fluidic chip immunofluorescence rapid detection kit as well as preparation and detection methods thereof

Abstract

The invention discloses a micro-fluidic chip immunofluerescence rapid detection kit as well as preparation and detection methods thereof. The kit comprises a micro-fluidic chip; the micro-fluidic chip comprises one or more detection units; the detection units are arranged on a radius axis of the micro-fluidic chip and are arranged in a divergence manner by taking the micro-fluidic chip as a center point; and a sample feeding groove, a fluorescence probe groove, a reaction detection groove, a waste liquid groove and microchannels which are mutually connected are sequentially arranged on the detection units from the center point of the chip to the edge. By using the kit, the dosage of a reagent is small, the integration degree is high, and a reaction systems is a homogeneous phase; and all the reagents are solidified in the chip in advance, and antigen-antibody reaction is carried out in a relatively closed space, so that crossed contamination and environmental pollution do not occur, and the kit has the advantages of high efficiency, high flux, high sensitivity, rapidness, sensitiveness, stability and the like.

Description

technical field [0001] The invention belongs to the field of immunological detection, and in particular relates to a microfluidic chip fluorescence immunological rapid detection kit and a preparation and detection method thereof. Background technique [0002] In recent years, the technology of microfluidic chips has developed rapidly, and its applications in medicine, life science and other fields have been continuously expanded. Microfluidic chip refers to the use of microfabrication technology to integrate the microchannel network structure and other functional components on a substrate of several square centimeters, and to control the fluid in the microchannel to realize sampling, dilution, mixing, reaction, The micro-total analysis system with multiple functions such as separation and detection has the remarkable advantages of miniaturization, integration, fast analysis speed, and less reagent consumption. [0003] Immunolabeling technology is based on the basic princip...

AI Technical Summary

Problems solved by technology

This patent has the following deficiencies: (1) The antigen and antibody reagents used still need to be stored in reagent bottles separately (same as the traditional method), and the liquid flow, mixing and reaction are driven by vacuuming during the test, and the reaction cannot be integrated; (2) The solid phase carrier used - polyester membrane instead of microporous plate (or test paper), the cost is high; (3) The pore size of polyester membrane affects the enrichment of antigen or antibody; (3) The heterogeneous reaction is carried out on the solid phase carrier The surface reacts, the reaction is insufficient, and the sensitivity and repeatability are limited

However, this technology needs to be matched with large-scale detection instruments, which is expensive, time-consuming, and consumes a large amount of reagents and samples.

[0009] "Research on microfluidic biochip system based on magnetic bead technology" ("Shanghai Jiaotong University" 2007) is to place immunomagnetic beads in microfluidic channels. Effects of exposure, antigens and antibodies do not guarantee a full and complete response

[0010] There are no more literature reports on the combination of microfluidic technology, magnetic particle immune technology and fluorescent immunological technology

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