Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biosensor based on platinum/silicon carbide nano material and application thereof

A biosensor and nanomaterial technology, applied in the analysis of materials, material electrochemical variables, material analysis by electromagnetic means, etc., can solve the problems of complex sample pretreatment process, poor reproducibility of measurement results, and complicated instrument operation, etc. Excellent electrocatalytic activity, rapid detection, and high sensitivity

Active Publication Date: 2017-08-25
云南省产品质量监督检验研究院
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these techniques have expensive instruments, complicated instrument operation, bulky instruments, long detection time, complicated sample pretreatment process, poor reproducibility and poor stability of measurement results.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biosensor based on platinum/silicon carbide nano material and application thereof
  • Biosensor based on platinum/silicon carbide nano material and application thereof
  • Biosensor based on platinum/silicon carbide nano material and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] First, 100mg of SiC was dissolved in 40mL of ultrapure water, and 100mg of glucose was dissolved in 40mL of ultrapure water. After ultrasonic dispersion, they were transferred to a 100mL stainless steel autoclave at 180°C for 8 hours, and then cooled to room temperature. Wash three times with ethanol centrifugation; then, add 40mL ethanol and 1mL 3-aminopropyltriethoxysilane, stir for 12 hours overnight, wash three times with ethanol centrifuge, and freeze-dry the sample to obtain SiC-NH 2 nanomaterials. Take 2.0 mg of the prepared SiC-NH 2 material, added to 2.0mL 0.45mM H 2 PtCl 6 solution, add 5.0mL pyridine and 1.0mL 0.01M sodium citrate to the mixed solution and sonicate for 10 minutes, finally, add 1.0mL 0.01M NaBH 4 The solution was stirred at room temperature for 10 hours, then centrifuged at 12000 rpm for 20 minutes, washed with water three times, and the volume was adjusted to 10 mg / mL to obtain Pt NP@SiC-NH 2 Composite materials.

[0035] First, take 1.0...

Embodiment 2

[0038] First, 100mg of SiC was dissolved in 40mL of ultrapure water, and 100mg of glucose was dissolved in 40mL of ultrapure water. After ultrasonic dispersion, they were transferred to a 100mL stainless steel autoclave at 180°C for 8 hours, and then cooled to room temperature. Wash three times with ethanol centrifugation; then, add 40mL ethanol and 1mL 3-aminopropyltriethoxysilane, stir for 12 hours overnight, wash three times with ethanol centrifuge, and freeze-dry the sample to obtain SiC-NH 2 nanomaterials. Take 2.0 mg of the prepared SiC-NH 2 material, added to 2.0mL 0.45mM H 2 PtCl 6 solution, add 5.0mL pyridine and 1.0mL 0.01M sodium citrate to the mixed solution and sonicate for 10 minutes, finally, add 1.0mL 0.01M NaBH 4 The solution was stirred at room temperature for 10 hours, then centrifuged at 12000 rpm for 20 minutes, washed with water three times, and the volume was adjusted to 10 mg / mL to obtain Pt NP@SiC-NH 2 Composite materials.

[0039] First, take 1.0...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biosensor based on a platinum / silicon carbide nano material and application thereof and belongs to the technical field of electrochemical detection. A preparation method of the biosensor comprises the following steps: firstly taking SiC, Glucost, ethanol, APTES, H2PtCl6, EDTA, NaOH, NaBH4, pyridine and sodium citrate as starting raw materials, and respectively preparing an SiC-NH2 nano material through a hydrothermal method and preparing a PtNp@SiC-NH2 nano composite material through a normal-temperature reduction method; and secondly immobilizing acetylcholinesterase (AChE) on the surface of a PtNP@SiC-NH2 modified glass carbon electrode through physical absorption by using chitosan (CS)-immobilized acetylcholinesterase to build an electrochemical biosensor. The sensor is applied to detection of methyl parathion pesticides and carbaryl pesticides. The biosensor provided by the invention has the following beneficial effects: through the technology with combination of electrochemistry and enzyme sensing, the high-sensitivity detection of two pesticides comprising methyl parathion and carbaryl can be successfully achieved; the biosensor is simple in detection method, low in sample consumption, rapid in response, high in sensitivity and good in stability.

Description

[0001] technology neighborhood [0002] Based on the combination process of electrochemical technology and immobilized enzyme, the present invention relates to the construction of a biosensor based on platinum / silicon carbide nanomaterials, which belongs to the neighborhood of electrochemical detection technology. Background technique [0003] In modern society, pesticides are widely used as insecticides to prevent and control insect pests on the one hand, and increase production, ensure harvests, and improve farmers' living standards on the other hand. There have also been problems with public health, the environment and food safety in modern agriculture caused by the extensive use of different types of pesticides. Therefore, in order to improve and ensure food quality and life safety, the highly sensitive detection of pesticide residues has become a hot spot of widespread concern for researchers. At present, the commonly used and relatively mature methods for the detection ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3278G01N27/48
Inventor 于毅涛
Owner 云南省产品质量监督检验研究院
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com