Pueraria lobata planting method by using tissue culture seedlings

Abstract

The invention discloses a Pueraria lobata planting method by using tissue culture seedlings. The planting method cultivates stem tip meristematic tissues of new buds of Pueraria lobata, can effectively eliminate diseases sources, and performs callus tissue, adventitious bud and small plant stage cultivation to acquire a virus-free Pueraria lobata provenance. The planting method is not limited by seasons, and can establish a rapid high efficient regeneration system which is easy to master, and is simple and saving. Glass cultivation bottles used in tissue culture have upper openings and lower openings which are formed by upper covers and lower covers; during callus tissue transferring and adventitious bud transferring, callus tissues and adventitious buds are taken out of the lower openings of the lower covers, and the result that the survival rate is decreased due to the fact that plant tissues in bottles are broken or damaged due to puling or tearing when callus tissues and adventitious buds are taken out of single upper openings of the conventional cultivation bottles can be avoided. The Pueraria lobata planting method by using tissue culture seedlings is simple to operate, can save soil resources, is easy to promote, and has excellent economic benefits and ecological benefits.

Description

technical field [0001] The invention relates to a plant planting method. More specifically, the present invention relates to a planting method of kudzu mirifica using tissue cultured seedlings. Background technique [0002] Pueraria mirifica is a perennial vine of the genus Pueraria in the family Fabaceae, also known as Pueraria lobata, kudzu vine, etc. In the field of traditional Chinese medicine, the root of Pueraria mirifica is customarily called Pueraria lobata. Pueraria lobata likes a warm and humid environment, and is suitable for neutral and acidic soils. The suitable growth temperature is 20°C to 30°C, and it can withstand high temperatures of 39°C and low temperatures of minus 20°C. The dried kudzu root is long cylindrical, the outer skin is grayish yellow, and the inner part is powdery and rich in cellulose. Pueraria lobata is produced in Hunan, Jiangxi, Zhejiang, Henan, Guangdong, Guangxi, Sichuan, Yunnan and other provinces and regions. It is a kind of commonly...

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Problems solved by technology

[0004] In recent years, with the improvement of people's living standards, people pay more and more attention to nutrition, health care, and health preservation. Pueraria lobata, which has the reputation of "Asian ginseng", has become more and more known to people, so the demand for kudzu It is also gradually increasing. The cultivation and planting of kudzu in my country is mainly concentrated in the southern region. The quality of kudzu cultivated by traditional planting methods cannot meet the current demand.

Moreover, the traditional planting method uses kudzu vines for direct planting. The vines have not grown into seedlings, and it is difficult to resist the germs in the soil. They are often easily infected with germs, resulting in uneven quality, which makes some kudzu. good traits, the harvested kudzu root usually carries pathogens and is of poor quality

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