Pueraria lobata planting method by using tissue culture seedlings

A planting method and technology of tissue culture seedlings, applied in the planting field of Pueraria mirifica, can solve problems such as unsatisfactory quality of Pueraria mirifica, inability to maintain properties of Pueraria mirifica, uneven quality of itself, etc., achieve good economic and ecological benefits, be easy to popularize, The effect of improving the germination rate

Inactive Publication Date: 2017-09-15
马山县盛世农业发展有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, with the improvement of people's living standards, people pay more and more attention to nutrition, health care, and health preservation. Pueraria lobata, which has the reputation of "Asian ginseng", has become more and more known to people, so the demand for kudzu It is also gradually increasing. The cultivation and planting of kudzu in my country is mainly concentrated in the southern region. The quality of kudzu cultivated by traditional planting methods cannot meet the current demand.
Moreover, the traditional planting method uses kudzu vines for direct planting. The vines have not grown into seedlings, and it is difficult to resist the germs in the soil. They are often easily infected with germs, resulting in uneven quality, which makes some kudzu. good traits, the harvested kudzu root usually carries pathogens and is of poor quality

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  • Pueraria lobata planting method by using tissue culture seedlings

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Embodiment 1

[0037] The invention provides a method for planting kudzu mirifica using tissue culture seedlings, comprising the following steps:

[0038] Step 1. Select a sandy loam field with deep soil layer, loose and fertile soil as the planting field, apply soil disinfectant, and after 5 to 7 days of soil disinfection, plow the land until the soil is soft and free of sticky lumps, add humus soil, and adjust The pH is 5.5-7.5.

[0039] Step 2. In the greenhouse, bury the strong kudzu root in the growth substrate, water it thoroughly, and spray the nutrient solution once every 3 days until new tender stems grow, wherein the growth substrate includes the following raw materials in parts by weight: river sand 70 ~80 parts, 15-20 parts of perlite, 10-15 parts of urea-formaldehyde foam, 12-16 parts of porous ceramsite, 8-12 parts of biochemical fulvic acid; the nutrient solution includes the following raw materials in parts by weight: 35-45 parts of plant peat , 15-20 parts of wheat bran, 4-...

Embodiment 2

[0058] The invention provides a method for planting kudzu mirifica using tissue culture seedlings, comprising the following steps:

[0059] Step 1. Select a sandy loam field with deep soil layer, loose and fertile soil as the planting field, apply soil disinfectant, and after 5 to 7 days of soil disinfection, plow the land until the soil is soft and free of sticky lumps, add humus soil, and adjust The pH is 5.5-7.5.

[0060] Step 2. In the greenhouse, bury the strong kudzu root in the growth substrate, water it thoroughly, and spray the nutrient solution once every 3 days until new tender stems grow, wherein the growth substrate includes the following raw materials in parts by weight: river sand 70 ~80 parts, 15-20 parts of perlite, 10-15 parts of urea-formaldehyde foam, 12-16 parts of porous ceramsite, 8-12 parts of biochemical fulvic acid; the nutrient solution includes the following raw materials in parts by weight: 35-45 parts of plant peat , 15-20 parts of wheat bran, 4-...

Embodiment 3

[0079] The invention provides a method for planting kudzu mirifica using tissue culture seedlings, comprising the following steps:

[0080] Step 1. Select a sandy loam field with deep soil layer, loose and fertile soil as the planting field, apply soil disinfectant, and after 5 to 7 days of soil disinfection, plow the land until the soil is soft and free of sticky lumps, add humus soil, and adjust The pH is 5.5-7.5.

[0081] Step 2. In the greenhouse, bury the strong kudzu root in the growth substrate, water it thoroughly, and spray the nutrient solution once every 3 days until new tender stems grow, wherein the growth substrate includes the following raw materials in parts by weight: river sand 70 ~80 parts, 15-20 parts of perlite, 10-15 parts of urea-formaldehyde foam, 12-16 parts of porous ceramsite, 8-12 parts of biochemical fulvic acid; the nutrient solution includes the following raw materials in parts by weight: 35-45 parts of plant peat , 15-20 parts of wheat bran, 4-...

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Abstract

The invention discloses a Pueraria lobata planting method by using tissue culture seedlings. The planting method cultivates stem tip meristematic tissues of new buds of Pueraria lobata, can effectively eliminate diseases sources, and performs callus tissue, adventitious bud and small plant stage cultivation to acquire a virus-free Pueraria lobata provenance. The planting method is not limited by seasons, and can establish a rapid high efficient regeneration system which is easy to master, and is simple and saving. Glass cultivation bottles used in tissue culture have upper openings and lower openings which are formed by upper covers and lower covers; during callus tissue transferring and adventitious bud transferring, callus tissues and adventitious buds are taken out of the lower openings of the lower covers, and the result that the survival rate is decreased due to the fact that plant tissues in bottles are broken or damaged due to puling or tearing when callus tissues and adventitious buds are taken out of single upper openings of the conventional cultivation bottles can be avoided. The Pueraria lobata planting method by using tissue culture seedlings is simple to operate, can save soil resources, is easy to promote, and has excellent economic benefits and ecological benefits.

Description

technical field [0001] The invention relates to a plant planting method. More specifically, the present invention relates to a planting method of kudzu mirifica using tissue cultured seedlings. Background technique [0002] Pueraria mirifica is a perennial vine of the genus Pueraria in the family Fabaceae, also known as Pueraria lobata, kudzu vine, etc. In the field of traditional Chinese medicine, the root of Pueraria mirifica is customarily called Pueraria lobata. Pueraria lobata likes a warm and humid environment, and is suitable for neutral and acidic soils. The suitable growth temperature is 20°C to 30°C, and it can withstand high temperatures of 39°C and low temperatures of minus 20°C. The dried kudzu root is long cylindrical, the outer skin is grayish yellow, and the inner part is powdery and rich in cellulose. Pueraria lobata is produced in Hunan, Jiangxi, Zhejiang, Henan, Guangdong, Guangxi, Sichuan, Yunnan and other provinces and regions. It is a kind of commonly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G9/10A01H4/00C05G3/00C05G3/04C05G1/00C05G3/80
CPCA01H4/001A01H4/008C05B1/02C05C3/00C05D9/00C05G3/00C05G3/80C05G5/20C05F11/02C05B7/00C05D9/02C05F5/002C05F11/00C05C5/04C05C11/00C05G1/00
Inventor 蒙有胜
Owner 马山县盛世农业发展有限责任公司
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