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Process for adsorbing heparin molecules through large-aperture resin

A technology of resin adsorption and large pore size, which is applied in the chemical industry, can solve the problems of low purity and low recovery rate, and achieve the effects of high purity of fine products, saving ethanol and low cost

Inactive Publication Date: 2017-09-19
南通游龙肠衣食品有限公司
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AI Technical Summary

Problems solved by technology

The most commonly used extraction method is salt hydrolysis or enzymatic hydrolysis-ion exchange-ethanol precipitation method, but these two methods generally have the problems of low recovery rate and low purity

Method used

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Examples

Experimental program
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Embodiment 1

[0020] A method for extracting heparin sodium from pig small intestine, comprising the steps of:

[0021] (1) Adsorption: Weigh 500kg of pig small intestinal mucosa, put it in a reaction pot, add water to dilute, adjust the pH value to 9.0-9.5 with 20% sodium hydroxide solution, heat to boiling, keep for 15min, cool down to 35°C, and at the same time, siphon the filtrate to In the reaction pool, add 10% D204 resin resin, keep stirring and absorb for 10h;

[0022] (2) Washing: take the adsorbed liquid in step (1) and let it stand for 30 minutes, then filter it, collect the resin, rinse the resin with water, and drain it for later use;

[0023] (3) Elution: Take the solid in step (2) and add 1mol / L sodium chloride to wash for 1 to 3 hours. After draining, add 4mol / L sodium chloride to 1 to 3 times the amount, stir and elute for 5 hours, and drain Then eluted twice, adding 4mol / L sodium chloride solution to reduce the amount by half each time, the elution time was 2h and 1h resp...

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Abstract

The iinvention discloses an extraction method for heparin sodium, belongs to the field of chemical engineering, and particularly relates to a process for adsorbing heparin molecules through large-aperture resin. The process comprises the following steps: (1) adsorption; (2) washing; (3) elution; (4) separation; (5) removal of proteins; (6) precipitation. According to a preparation method provided by the invention, readily available porcine intestinal mucosa is used as a raw material, so that the cost is low. A polyacrylonitrile film is used for separation, so that a lot of ethyl alcohol can be saved, and the production cost can be reduced; meanwhile, a prepared heparin sodium quality product is high in purity, and the activity of heparin is guaranteed.

Description

technical field [0001] The invention belongs to the field of chemical industry, and relates to a method for extracting heparin sodium, in particular to a technique for adsorbing heparin molecules with a large-pore resin. Background technology [0003] [0002] Heparin is mainly extracted from the mucous membrane of bovine lung or pig small intestine, and is a mucopolysaccharide sulfate composed of glucosamine, L-iduroside, N-acetylglucosamine and D-glucuronic acid alternately. Heparin combines with antithrombin III to expose the arginine reaction center, and combines with activated coagulation factors to inactivate, mainly inactivating factors Xa and IIa. Heparin is mainly used for the prevention and treatment of thromboembolic diseases, such as myocardial infarction, pulmonary embolism, cerebrovascular embolism, peripheral venous thrombosis, etc., and can prevent the formation and expansion of thrombus. It can also be used in the early stage of DIC and other anticoagulatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10
CPCC08B37/0075C08B37/0003
Inventor 陈文龙张宏宋磊
Owner 南通游龙肠衣食品有限公司
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