Phytopathogen antagonistic bacterium and its application in plant disease control

A plant disease and disease technology, which is applied to a plant pathogenic bacteria antagonism and its application in the prevention and control of plant diseases, can solve the problems of destroying soil microecology, adverse effects on farmland environment, and high cost

Active Publication Date: 2017-09-22
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of chemical agents for prevention and control has problems such as high cost and environmental pollution. Moreover, the agents can also inhibit the growth of beneficial bacteria while killing pathogenic bacteria, destroying the micro-ecology of the soil, and causing great adverse effects on the farmland environment.
There have been many reports on the use of endophytic bacteria to control plant diseases, but there has been no report on the use of cassava endophytic bacteria to control cassava diseases at home and abroad.

Method used

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  • Phytopathogen antagonistic bacterium and its application in plant disease control
  • Phytopathogen antagonistic bacterium and its application in plant disease control
  • Phytopathogen antagonistic bacterium and its application in plant disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, screening and identification of phytopathogen antagonistic bacteria

[0020] 1. Screening of antagonistic bacteria of plant pathogens

[0021] The bacterial strain of the present invention is isolated from the inside of healthy cassava leaves (the cassava variety is Huanan No. 8). The specific method is: take 1g of leaves, cut them into small pieces of 5mm × 5mm, put them into 70% alcohol, and leave them for 60s. Finally, rinse once with sterile water, then put it into 3.25% sodium hypochlorite, and rinse repeatedly with sterile water after 5 minutes. Put it into a sterilized mortar with 9 mL of sterile water, add a little sterilized quartz sand to grind evenly, and let it stand for 15 minutes. Take 1mL and dilute to 10 -3 、10 -4 、10 -5 Concentration gradient, take 0.1mL from each concentration gradient suspension, and spread it on NA medium with a sterile applicator (recipe: peptone 5g, yeast extract 1g, beef extract 3g, agar powder 15g, glucose 10g, ...

Embodiment 2

[0035] Embodiment 2, Paenibacillus sp. (Paenibacillus sp.) CEB33 CGMCC No.12719 antibacterial spectrum determination

[0036] According to the method in Example 1, CEB33 was co-cultured with cassava bacterial wilt (Xanthomonasaxonopodis pv.manihotis, purchased from China Agricultural Microorganism Culture Collection Management Center, preservation number ACCC03517) ( figure 1 ). The results of the antagonistic effect of the strain on cassava bacterial wilt were as follows: figure 1 As shown, the results showed that the screened strains had a strong antagonistic effect on cassava bacterial wilt. figure 1 Middle II is the confrontation culture test between CEB33 and cassava bacterial wilt, and I is the growth of control cassava bacterial wilt. This bacterial strain has good inhibitory effect to cassava bacterial wilt, and the diameter of the inhibition zone is 1.7cm (such as figure 1 shown).

[0037] Use PDA medium plate (recipe: peeled potato 200g, glucose 20g, agar 20g, af...

Embodiment 3

[0038] Example 3, Endogenous Determination of Paenibacillus sp. CEB33 CGMCC No.12719

[0039] 1. Acquisition of ampicillin-resistant bacteria from Paenibacillus sp. CEB33 CGMCC No.12719

[0040] Inoculate Paenibacillus sp. CEB33 into LB liquid medium containing 10 μg / mL ampicillin (Amp) (recipe: tryptone 10 g, sodium chloride 10 g, yeast extract 5 g, dilute to 1000 mL with distilled water, adjust pH to 7.0, sterilized at 121°C for 20 minutes), cultured by shaking for about 48 hours until the bacterial liquid became cloudy, inoculated a small amount of cultured bacterial liquid into LB liquid medium containing 15 μg / mL ampicillin (Amp), and cultured by shaking until the bacterium becomes cloudy. According to this method, the concentration of ampicillin was gradually increased for cultivation, and finally Paenibacillus that could grow stably on the LB medium containing ampicillin at a concentration of 300 μg / mL, and whose colony morphology and antagonism to pathogenic bacteria ...

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Abstract

The invention discloses a phytopathogen antagonistic bacterium and its application in plant disease control. The phytopathogen antagonistic bacterium refers to paenibacillus (Paenibacillus sp.) CEB33 CGMCC No.12719. The phytopathogen antagonistic bacterium, namely the paenibacillus (Paenibacillus sp.) CEB33 CGMCC No.12719 can control plant diseases caused by cassava xanthomonas axonopodis (Xanthomonas axonopodis pv.manihotis), cassava corynespora cassiicola (Corynespora cassiicola), cassava colletotrichum gloeosporioides (Colletotrichum gloeosporioides) and cassava phytophthora palmivora (Phytophthora palmivora); especially, the phytopathogen antagonistic bacterium and cassava can coexist, and the phytopathogen antagonistic bacterium stably survives in the cassava for controlling cassava xanthomonas axonopodis.

Description

technical field [0001] The invention relates to a plant pathogen antagonistic bacteria and its application in preventing and treating plant diseases. Background technique [0002] Plant endophytic bacteria refer to bacteria that can enter living plant tissues during their lifetime or at least during a certain period of their lifetime, and their existence does not significantly change the phenotypic characteristics and functions of plant tissues. In 1926, Perotti discovered the existence of endophytic bacteria in the root tissues of many healthy plants, and then the research in this area made great progress, and a variety of endophytic bacteria were found in different tissues of many plants. These endophytic bacteria use plant tissues as habitats, and often play a role in promoting growth, preventing pests, nitrogen fixation, adversity resistance (such as drought, etc.), and animal (insect, herbivorous mammals, etc.) ingestion effects on host plants, etc. It can produce acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 黄贵修陈奕鹏李超萍时涛霍姗姗刘先宝蔡吉苗杨杨李博勋
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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