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A kind of balsa-lysozyme anti-infection dressing and its preparation method and application

A lysozyme and anti-infection technology, applied in medical science, absorbent pads, bandages, etc., can solve the problem of limited effect of free lysozyme, and achieve the effect of low price, low cost and easy access

Inactive Publication Date: 2020-06-19
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, free lysozyme has limited effect on Gram-negative bacilli due to the presence of lipopolysaccharide components that protect the bacterial cell wall

Method used

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  • A kind of balsa-lysozyme anti-infection dressing and its preparation method and application
  • A kind of balsa-lysozyme anti-infection dressing and its preparation method and application
  • A kind of balsa-lysozyme anti-infection dressing and its preparation method and application

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preparation example Construction

[0035] The invention discloses a preparation method of a balsa-lysozyme anti-infection dressing, which comprises the following steps:

[0036] Step 1, prepare delignified balsa wood: choose a density of 150-170kg / m 3The balsa wood is punched with a puncher to make a disc with a diameter of 0.6mm and a thickness of 0.8mm; dried at 102-108°C for 20-28 hours, and then soaked in a mass concentration of 0.5%-1.5 % NaClO 2 -React in acetate buffer (PH4.6, 80°C) for 8-16h; wash the soaked balsa wood carefully with deionized water and mix acetone with ethanol-ethanol (1:1, v / v)-acetone Three steps of sequential drying, each step of 10min, repeated three times to prepare delignified balsa wood;

[0037] Step 2. Dopamine gel-linked lysozyme: Dissolve hydrochloric acid in deionized solution, and then add dopamine powder to prepare a Tris-dopamine solution of 1.8-2.2mg / ml, pH 8.4-8.6, within the concentration and pH range In the process, the dopamine adhesion efficiency is maximized; t...

Embodiment 1

[0039] A preparation method of balsa-lysozyme anti-infection dressing, comprising the following steps:

[0040] Step 1, preparing delignified balsa wood: choosing a density of 150kg / m 3 The balsa wood is punched with a puncher to make a disc with a diameter of 0.6mm and a thickness of 0.8mm; dry it at 105°C for 24 hours, and then soak it in 1% NaClO 2 -React in acetate buffer solution (PH4.6, 80° C.) for 12 hours. The extracted sample was carefully cleaned with deionized water and then dried according to the three-step sequence of ethanol-ethanol-acetone mixture (1:1, v / v)-acetone, each step was 10 minutes, and repeated three times to prepare delignified balsa wood;

[0041] Step 2, dopamine gel-linked lysozyme: Weigh 131.14mg Tris hydrochloric acid and dissolve it in 100ml deionized, then add 200mg dopamine powder to prepare a 2mg / ml Tris-dopamine solution with a pH of 8.5. Then the delignified balsa wood was soaked in dopamine solution for 12 hours, placed on a shaker at 3...

Embodiment 2

[0043] A preparation method of balsa-lysozyme anti-infection dressing, comprising the following steps:

[0044] Step 1, preparing delignified balsa wood: choosing a density of 170kg / m 3 The balsa wood is punched with a hole punch to make a disc with a diameter of 0.6mm and a thickness of 0.8mm; dried at 102-108°C for 20 hours, and then soaked in NaClO with a mass concentration of 1.5% 2 -React in acetate buffer solution (PH4.6, 80°C) for 8 hours; carefully wash the extracted sample with deionized water and then dry it according to the three-step sequence of ethanol-ethanol-acetone mixture (1:1, v / v)-acetone, Each step is 10min, repeated three times to prepare delignified balsa wood;

[0045] Step 2, dopamine gel-linked lysozyme: Weigh Tris hydrochloric acid and dissolve it in deionized, then add dopamine powder to prepare a Tris-dopamine solution with a pH of 2.2mg / ml and a pH of 8.4; then soak the delignified balsa wood in In the Tris-dopamine solution for 10 hours, place i...

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Abstract

The invention discloses a balsa-lysozyme anti-infection dressing, and a preparation method and application thereof. The preparation method comprises the following steps of selecting balsa; performing hole making by a hole making device for preparing the balsa into round sheets; performing drying treatment on the round sheets; then, performing soaking treatment; carefully cleaning the soaked balsa by deionized water; performing drying treatment; performing preparation to obtain balsa subjected to lignin removal; preparing Tris-dopamine solution; then, soaking the balsa subjected to lignin removal into the Tris-dopamine solution; performing soaking treatment; preparing a lysozyme solution; cleaning the dopamine soaked balsa; soaking the balsa into the lysozyme solution; performing soaking treatment in a table concentrator; preparing the balsa-lysozyme anti-infection dressing. In a form of crosslinking the lysozyme by the dopamine, the lysozyme is sufficiently adhered into multiple apertures of the balsa, a relative stable release microenvironment is provided, so that a certain sterilization effect is also achieved on gram-negative bacteria.

Description

technical field [0001] The invention belongs to the field of medicine preparation, and in particular relates to a balsa-lysozyme anti-infection dressing and its preparation method and application. Background technique [0002] For patients with large and severe skin defects, it is urgent to quickly and effectively seal the wound by various means to prevent bacterial invasion and prevent loss of body fluids, water electrolytes and energy. Wound dressings can rebuild the skin barrier, accelerate wound healing, and prepare for later surgery. An ideal wound dressing should have the following characteristics: good mechanical properties, proper water vapor permeability and excellent biocompatibility. More importantly, infection, inflammatory response disorders, etc. will cause wound healing to slow down or even not heal. An ideal wound dressing should also be able to provide a sterile microenvironment suitable for local wound growth. And natural materials such as chitin and chit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/40A61L15/38A61L15/46A61L15/20
CPCA61L15/20A61L15/38A61L15/40A61L15/46A61L2300/254A61L2300/404
Inventor 周代君罗高兴邢梦秋吴军
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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