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Biomass charcoal based microbial agent for preventing and controlling watermelon fusarium wilt as well as preparation method and application thereof

A watermelon wilt and microbial agent technology, applied in the direction of botanical equipment and methods, applications, chemical instruments and methods, etc., can solve complex and difficult to receive effects and other problems, and achieve simple preparation methods, strong prevention and treatment function, the effect of improving survival time

Active Publication Date: 2017-11-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The generation of continuous cropping obstacles involves many complex biological and abiotic factors such as crops, soil, environment, etc., and the interaction of these factors makes solving this problem more complicated. Any single measure or a few measures are very important. Difficult to get the desired effect

Method used

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  • Biomass charcoal based microbial agent for preventing and controlling watermelon fusarium wilt as well as preparation method and application thereof
  • Biomass charcoal based microbial agent for preventing and controlling watermelon fusarium wilt as well as preparation method and application thereof
  • Biomass charcoal based microbial agent for preventing and controlling watermelon fusarium wilt as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Preparation of Biomass Charcoal-Based Microbial Inoculant for Control of Watermelon Fusarium Wilt

[0038]Step 1: Take the purified biocontrol bacteria (Bacillus tequila and Bacillus death valley), insert them into LB liquid medium, and cultivate them on a shaker (150 rpm) at 37±1°C for 48 hours, and the obtained bacteria content is 10 12 cfu / mL biocontrol bacterial fermentation broth, for later use.

[0039] Step 2: Using rice husk as raw material, use a high-temperature open-type tube furnace at 500°C (in the specific implementation process, the temperature of the tube furnace can be controlled within the range of 400-700°C) to prepare biochar under anaerobic conditions, and crush it 40-mesh sieve, sterilized in a sterilizing pot at 121°C for 1h (in the specific implementation process, it can be sterilized for 1-2h according to the actual situation), and then taken out to obtain rice husk biochar, which is set aside.

[0040] Step 3, take a total of 20Kg ...

Embodiment 2

[0043] The shelf life test of embodiment 2 bacterial agents

[0044] (1) Dilute the biocontrol bacterial fermentation broth obtained in Step 1 of Example 1 with physiological saline to make it the same as the initial average amount of the biomass charcoal-based microbial agent obtained in Step 3, and the bacterial content is about 5.6 billion cfu / g, sealed and stored at room temperature.

[0045] (2) Determination of the number of biocontrol bacteria in biocontrol bacterial fermentation liquid and biomass charcoal-based microbial agent: take 50 mL of biocontrol bacterial fermentation liquid (or 50 g of biomass charcoal-based microbial agent), add sterile water to dilute to 1000 mL, placed in a shaker at 30°C and 200r / min for 30 min; take 10 mL of the solution into a 100 mL volumetric flask, set the volume to 100 mL, and then dilute 10 times for 5 times, then take 100uL and spread it on an LB plate, 30 Cultivate upside down for 12 hours at ℃, count the number of colonies, and...

Embodiment 3

[0048] Effect on soil enzyme activity after the application of embodiment 3 biomass charcoal-based microbial bacterial agent

[0049] (1) In order to eliminate the interference of miscellaneous bacteria in the soil, the Bacillus in the biochar-based microbial agent was first marked with antibiotics: the Bacillus was inoculated on an LB plate containing 1 μg / mL rifampicin, and the rifampicin was gradually increased. Level concentration to 300 μ g / mL, screen out the antibiotic-resistant bacterial strain that can grow stably and the physiological characteristic is consistent with original bacterial strain, prepare biomass charcoal-based microbial bacterial agent according to the step of embodiment 1;

[0050] (2) Set 2 processes:

[0051] A. Take 10 kg of field soil, add the biomass charcoal-based microbial agent obtained in step (1) according to the proportion of 2% by mass, mix well and place it at room temperature for sealed storage;

[0052] B. Resistant bacterial strains pr...

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Abstract

The invention discloses a biomass charcoal based microbial agent for preventing and controlling watermelon fusarium wilt as well as a preparation method and application thereof. The microbial agent contains the following components: biomass charcoal, attapulgite, watermelon fusarium wilt biocontrol bacterium, calcium superphosphate or nitrohumic acid and sterile water. The preparation method for the microbial agent comprises the following steps: absorbing the biocontrol bacterium solution to the biomass charcoal, and then adding attapulgite and calcium superphosphate or nitrohumic acid and uniformly mixing, adding a defined amount of sterile water, blending, pelletizing and drying in a dry ventilating place under a natural condition, thereby acquiring the microbial agent. The viable count in the microbial agent is more than or equal to 50 hundred million cfu / g, the diameter of the grain is 3-6mm and pH value is 6.5-7.5. The microbial agent disclosed by the invention has the functions of improving chemical and physical properties of soil, promoting soil enzyme activity, promoting crop growth, increasing crop output, and the like, and has an excellent function for preventing and controlling the watermelon fusarium wilt.

Description

technical field [0001] The invention relates to a microbial inoculum loaded with biomass charcoal in the field of agricultural technology, in particular to a method for preparing a solid microbial inoculum suitable for various facility crops and its application in preventing and treating wilt of watermelon. Background technique [0002] In recent years, my country's protected vegetables have developed rapidly, with a planting area of ​​58.326 million mu. Under protected cultivation conditions, long-term surface covering cultivation has changed the original ecological environment of the soil. The soil is often in a closed environment with high temperature, high humidity, high evaporation, and no rainwater leaching. In the environment of cyclic metabolism, the physical and chemical properties and biological characteristics of soil change, soil compaction, porosity decrease, soil salt surface accumulation phenomenon is obvious, and soil quality continues to deteriorate and intens...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40A01G13/00C09K101/00
CPCA01G13/00C09K17/40C09K2101/00
Inventor 余向阳张猛冯发运王琼
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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