Method for breeding of specific-pathogen-free parent penaeus vannamei

A technology for broodstock and prawns is applied in the field of broodstock cultivation without specific pathogens of Litopenaeus vannamei. Effect

Inactive Publication Date: 2017-11-21
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the breeding process of broodstock, it is usually necessary to change the water by 100% or more every day, and in order to ensure that the broodstock will not be affected by factors such as the temperature difference of the water change, it is often necessary to heat up or cool down the newly taken water. This method is a great waste of water and electricity resources; transforming and degrading ammonia nitrogen and nitrite nitrogen in the water body with microorganisms is another commonly used method to ensure water quality in the breeding process of broodstock, but in the general biodegradation process, The rate of degradation of ammonia nitrogen into nitrite nitrogen is much faster than the process of conversion from nitrite nitrogen to nitrite nitrogen and denitrification of nitrite nitrogen and nitrate nitrogen. Therefore, during the breeding process of broodstock, especially in the early stage, nitrite nitrogen Exceeding the standard is still the main factor affecting the health of broodstock
Aquatic plants or algae can effectively absorb eutrophication of nitrogen and phosphorus in the water body. Using aquatic plants or algae to establish a circulating water system for broodstock cultivation can achieve efficient ecological control of water quality. At present, there is no relevant report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Use seawater with a salinity of 28‰~30‰, a temperature of 27~28°C, and a dissolved oxygen concentration above 3mg / L. After filtering and purifying the seawater, it is disinfected with ozone, and then used for secondary disinfection with ultraviolet light. Finally, oxidizing agent is added. Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Choose one or more combinations of oysters, clamworms, and squids as bait, and use 60Co-γ irradiation after freezing. The irradiation dose exceeds 10.4kGy to sterilize the frozen bait. After thawing in the environment of 4~15℃.

[0021] Discharge the cultivation water after broodstock cultivation, remove the particulate matter in the water through filtration, and then remove the organic matter in the water body through the foam separator to purify the cultivation water; injec...

Embodiment 2

[0023] Use seawater with a salinity of 30‰~32‰, a temperature of 27~28°C, and a dissolved oxygen concentration of more than 3mg / L. After filtering and purifying the seawater, disinfect it with ozone, then use ultraviolet rays for secondary disinfection, and finally add oxidizing Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Nereis worm was selected as bait, frozen and irradiated with 60Co-γ. The irradiation dose exceeded 10.4kGy to sterilize the frozen bait. Before feeding, the frozen bait was thawed in an environment of 6-8°C.

[0024] Discharge the cultivation water after broodstock cultivation, remove the particulate matter in the water through filtration, and then remove the organic matter in the water body through the foam separator to purify the cultivation water; inject the purified cultivation water into t...

Embodiment 3

[0026] Use seawater with a salinity of 29‰~30‰, a temperature of 27~28℃, and a dissolved oxygen concentration above 3mg / L. The seawater is filtered and purified, then disinfected with ozone, and then used for secondary disinfection with ultraviolet light. Finally, oxidizing agent is added. Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Choose squid as bait, after freezing, use 60Co-γ irradiation, and the irradiation dose exceeds 10.4kGy to sterilize the frozen bait.

[0027] Discharge the cultivation water after broodstock cultivation, filter to remove particulate matter in the water, and then remove organic matter in the water body through a foam separator to purify the cultivation water; inject the purified cultivation water into the Gracilaria culture device, and use Gracilaria Absorb eutrophic nitrogen and pho...

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PUM

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Abstract

The invention discloses a method for breeding of specific-pathogen-free parent penaeus vannamei. The method includes the steps of early-stage preparation of for-breeding water, preparation and feeding of bait, and for-breeding water ecological purification cycle; filtered, purified and disinfected seawater serves as for-breeding water, the frozen bait is used for feeding penaeus vannamei after being subjected to ray disinfection, then the for-breeding water is injected into an aquatic plant cultivation device after being purified, after cultivation, the obtained water is disinfected and then injected into a parent penaeus vannamei pool, and water cycle is formed. The method avoids occurrence of the phenomenon that raw seawater carries pathogens and thus infects parent penaeus vannamei, provides the bait which is rich in nutrition, can ensure bio-safety and is conveniently frozen and stored for a long time for parent penaeus vannamei breeding, and provides a safe nutrition guarantee for the high-efficiency healthy-larva production of the parent penaeus vannamei. A water cycle system using aquatic plants or algae to control the water quality can achieve the efficient ecological control over the water quality, the quality of the parent penaeus vannamei is ensured, and the production efficiency is improved.

Description

technical field [0001] The invention belongs to the field of aquaculture and relates to a method for cultivating broodstock of Litopenaeus vannamei without specific pathogens. Background technique [0002] Shrimp farming is the pillar industry of aquaculture in my country, and Litopenaeus vannamei is the leading species of aquaculture. However, epidemic diseases caused by pathogenic microorganisms often have a fatal blow to the shrimp farming industry. Broodstock is infected with pathogens by eating bait carrying viruses and other pathogenic pathogenic microorganisms. The virus then spreads vertically and horizontally from broodstock to larvae, and then spreads horizontally from shrimp seedlings to shrimp seedlings, causing a large-scale epidemic of infectious diseases. one of the main factors. Studies have shown that oysters, clamworms, and squid are the intermediate hosts of pathogenic microorganisms such as shrimp viruses, while oysters, clamworms, and squid are the mos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59A01K63/04A23K50/80A23K10/22A23K10/20C02F9/14C02F103/20
CPCA01K61/59A01K63/04A23K10/20A23K10/22A23K50/80C02F1/001C02F1/32C02F3/32C02F9/00C02F2103/20C02F2301/08C02F2303/04Y02A40/81
Inventor 赵永贞陈晓汉刘青云陈秀荔李强勇谢达祥王卉
Owner GUANGXI ACADEMY OF FISHERY SCI
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