Method for breeding of specific-pathogen-free parent penaeus vannamei
A technology for broodstock and prawns is applied in the field of broodstock cultivation without specific pathogens of Litopenaeus vannamei. Effect
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Embodiment 1
[0020] Use seawater with a salinity of 28‰~30‰, a temperature of 27~28°C, and a dissolved oxygen concentration above 3mg / L. After filtering and purifying the seawater, it is disinfected with ozone, and then used for secondary disinfection with ultraviolet light. Finally, oxidizing agent is added. Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Choose one or more combinations of oysters, clamworms, and squids as bait, and use 60Co-γ irradiation after freezing. The irradiation dose exceeds 10.4kGy to sterilize the frozen bait. After thawing in the environment of 4~15℃.
[0021] Discharge the cultivation water after broodstock cultivation, remove the particulate matter in the water through filtration, and then remove the organic matter in the water body through the foam separator to purify the cultivation water; injec...
Embodiment 2
[0023] Use seawater with a salinity of 30‰~32‰, a temperature of 27~28°C, and a dissolved oxygen concentration of more than 3mg / L. After filtering and purifying the seawater, disinfect it with ozone, then use ultraviolet rays for secondary disinfection, and finally add oxidizing Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Nereis worm was selected as bait, frozen and irradiated with 60Co-γ. The irradiation dose exceeded 10.4kGy to sterilize the frozen bait. Before feeding, the frozen bait was thawed in an environment of 6-8°C.
[0024] Discharge the cultivation water after broodstock cultivation, remove the particulate matter in the water through filtration, and then remove the organic matter in the water body through the foam separator to purify the cultivation water; inject the purified cultivation water into t...
Embodiment 3
[0026] Use seawater with a salinity of 29‰~30‰, a temperature of 27~28℃, and a dissolved oxygen concentration above 3mg / L. The seawater is filtered and purified, then disinfected with ozone, and then used for secondary disinfection with ultraviolet light. Finally, oxidizing agent is added. Chlorine is used for disinfection. After disinfection, sodium thiosulfate is used to remove residual oxidative chlorine, and the disinfected seawater is injected into the broodstock pond as water for cultivation. Choose squid as bait, after freezing, use 60Co-γ irradiation, and the irradiation dose exceeds 10.4kGy to sterilize the frozen bait.
[0027] Discharge the cultivation water after broodstock cultivation, filter to remove particulate matter in the water, and then remove organic matter in the water body through a foam separator to purify the cultivation water; inject the purified cultivation water into the Gracilaria culture device, and use Gracilaria Absorb eutrophic nitrogen and pho...
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