High-yield extraction method of salicin in branches and leaves of white willows

An extraction method and technology of salicin, which is applied in the field of high-yield extraction of salicin in white willow branches and leaves, can solve the problems of low product price, high overall cost, and high price, and achieve improved extraction rate, sufficient extraction, and increased yield. rate effect

Inactive Publication Date: 2017-12-19
安徽龙津生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The preparation of salicin by chemical synthesis method uses various organic intermediates, the raw materials are not easy to obtain, and some organic solvents are used, the price is high, so the overall cost is high. serious pollution
[0008] At present, the mai...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A high-yield extraction method for salicin in white willow branches and leaves, comprising the following steps:

[0034] (1) Crush: Put the white willow leaves under the ultraviolet wavelength of 240nm and the irradiation intensity of 100μW / cm 2 Ultraviolet radiation treatment under the condition of 30min, and then under the condition of pressure of 1MPa, airflow crushing, through a 400 mesh sieve, to obtain the white willow branches and leaves fine powder;

[0035] (2) Pretreatment: Put the fine powder of willow branches and leaves in liquid nitrogen and quickly freeze it at an ultra-low temperature of -40°C for 5 minutes. After taking it out quickly, thaw it in a microwave with a microwave frequency of 900MHz and a microwave power of 200W for 4 minutes, and freeze-thaw in this way Cycle through 2 times;

[0036] (3) Extraction and concentration: add the fine powder of willow branches and leaves into the ethanol solution with a volume fraction of 80% according to the ...

Embodiment 2

[0044] A high-yield extraction method for salicin in white willow branches and leaves, comprising the following steps:

[0045] (1) Crushing: the white willow branches and leaves are irradiated at a wavelength of 255nm and an irradiation intensity of 120μW / cm 2 Ultraviolet radiation treatment under the conditions of 25min, and then under the condition of pressure of 1.2MPa, airflow pulverization, through a 450 mesh sieve, to obtain the white willow branches and leaves fine powder;

[0046] (2) Pretreatment: Put the fine powder of willow branches and leaves in liquid nitrogen and quickly freeze it at an ultra-low temperature of -45°C for 4 minutes. After taking it out quickly, microwave it at a microwave frequency of 950MHz and a microwave power of 250W for 3 minutes. So freeze-thaw cycle processing 3 times;

[0047] (3) Extraction and concentration: according to the solid-liquid ratio of 1:12, add the fine powder of white willow branches and leaves into the ethanol solution w...

Embodiment 3

[0055] A high-yield extraction method for salicin in white willow branches and leaves, comprising the following steps:

[0056] (1) Crush: Put the white willow branches and leaves under the ultraviolet wavelength of 270nm and the irradiation intensity of 150μW / cm 2 Ultraviolet radiation treatment under the conditions of 15min, and then under the condition of pressure of 1.5MPa, airflow crushing, through a 500 mesh sieve, to obtain the white willow branches and leaves fine powder;

[0057] (2) Pretreatment: Put the fine powder of willow branches and leaves in liquid nitrogen and quickly freeze it at an ultra-low temperature of -50°C for 3 minutes. After taking it out quickly, microwave it at a microwave frequency of 1000MHz and a microwave power of 300W for 2 minutes. Freeze-thaw cycle treatment 3 times;

[0058] (3) Extraction and concentration: according to the solid-liquid ratio of 1:15, add the fine powder of white willow branches and leaves into the ethanol solution with ...

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PUM

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Abstract

The invention discloses a high-yield extraction method of salicin in branches and leaves of white willows. The method includes the steps of: crushing, pre-treatment, extraction, concentration, adsorption separation, decoloration, concentration crystallization, etc. In the method, before extraction, the branches and leaves of white willows are treated through ultraviolet irradiation, gas flow pulverization, liquid nitrogen freezing and microwave de-freezing, so that the cell wall structure of the branches and leaves of white willows is effectively damaged while active components in the cells are not damaged, thus remaining the nutrients as most as possible and bringing convenience to dissolution-out of the salicin in subsequent extraction step. In the extraction method, through treatment successively carried out with low-frequency ultrasonic wave, high-frequency ultrasonic wave and medium-frequency ultrasonic wave, cell tissue of the branches and leaves of white willows is further subjected to wall disruption or deformation, so that the active components are extracted more completely and extraction rate of the salicin is greatly increased; and then a filter residue of the branches and leaves of white willows is processed in a high-voltage pulse electric field, so that dissolution-out of the salicin in the filter residue of the branches and leaves of white willows is further promoted, thereby greatly increasing the yield of the salicin.

Description

technical field [0001] The invention belongs to the field of biological extraction, and in particular relates to a high-yield extraction method for salicin in white willow branches and leaves. Background technique [0002] Salicin, the English name is D(-)-Salicin, another name is D-(-)-salicin, the molecular formula is C13H18O7, the CAS number is 138-52-3, the molecular weight is 286.28, and the melting point is 199-202℃. [0003] Salicin is white crystal; taste bitter; specific optical rotation [α] 厍-45.6° (0.6 g / 100 cm 3 Anhydrous ethanol); soluble in water, easily soluble in boiling water, insoluble in ethanol (1:90), insoluble in ether or chloroform, but soluble in alkaline solution, pyridine or glacial acetic acid. Its aqueous solution has a neutral reaction, and there is no free phenolic hydroxyl group in the molecule, which belongs to the phenolic glycoside compound. Hydrolyzed by dilute acid or amygdalin to generate glucose and salicyl alcohol. The molecular form...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
CPCC07H1/08C07H15/203
Inventor 钟玉林
Owner 安徽龙津生物科技有限公司
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