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Preparation method and application of lung matrix hydrogel and medicine

A hydrogel and matrix technology, applied in the field of lung matrix hydrogel preparation, can solve the problems of poor selectivity and large side effects, achieve the effects of mild implementation conditions, reduce lung damage, and reduce excessive cell apoptosis

Inactive Publication Date: 2017-12-22
中国人民解放军西部战区总医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a preparation method, application and medicine of lung matrix hydrogel, aiming to solve the problem of large side effects and poor selectivity in regulating the apoptosis process of alveolar epithelial cells in APALI by using medicine etc.

Method used

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  • Preparation method and application of lung matrix hydrogel and medicine
  • Preparation method and application of lung matrix hydrogel and medicine
  • Preparation method and application of lung matrix hydrogel and medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of lung matrix hydrogel, comprising the following steps:

[0032] The rat decellularized lung tissue is pulverized, and then added into hydrochloric acid-pepsin solution at a ratio of 8mg / ml-12mg / ml for dissolution.

[0033] Add 0.05M-0.15M sodium hydroxide and 8×PBS-12×PBS to the solution after dissolving to adjust to physiological state (at the time of physiological state, pH is about 7.4, hydrogel concentration is about 8mg / ml); Under the condition of 37°C until a jelly-like lung matrix hydrogel is formed.

[0034] Wherein, the preparation method of rat decellularized lung tissue is as follows:

[0035] 1) After the rat is under general anesthesia, the whole body is perfused with heparin, and the complete heart and lung tissue of the rat is taken.

[0036] 2) Insert the trocar into the pulmonary artery through the right ventricle and fix it, set the speed of the peristaltic pump to 4-6ml / min, and pre-fill 0.1% sodium lauryl sulfate to the entir...

Embodiment 2

[0040] On the basis of Example 1, further, after general anesthesia of the rat, the abdominal aorta was intubated and 50 units / mL of heparin was used to perfuse the whole body, and the complete heart and lung tissue of the rat was obtained. Insert the trocar into the pulmonary artery through the right ventricle and fix it. The peristaltic pump adopts a card-type peristaltic pump. The peristaltic pump prefills 0.1% sodium lauryl sulfate to the entire perfusion pipeline at a speed of 5ml / min. Sequentially perfuse with 0.1% sodium lauryl sulfate for 2 hours, deionized water for 15 minutes, 1% Triton X-100 solution for 10 minutes, and double-antibody-added PBS for 72 hours until the tissue is transparent.

Embodiment 3

[0042] On the basis of Example 1, further, the step of comminuting the rat decellularized lung tissue is as follows: after the rat decellularized lung tissue is freeze-dried at a low temperature in a low-temperature freeze-dryer, it is ground into a powder with a pulverizer, and then powdered with ethylene oxide Alkanes for disinfection. Prepare the hydrochloric acid-pepsin solution according to the ratio of 10mg:10ml, add the rat decellularized lung tissue powder into the hydrochloric acid-pepsin solution according to the ratio of 10mg / ml, stir slowly at room temperature for 48 hours until dissolved, and centrifuge at 4°C and 10000rpm / min Remove undissolved substances; adjust to physiological state (PH=7.4) by adding 0.1M sodium hydroxide and 10×PBS, and place at 37° C. until gelatinous lung matrix hydrogel is formed.

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Abstract

The invention provides a preparation method and application of lung matrix hydrogel and a medicine, and aims to solve the problem that no effective medicine is available to adjust and control apoptosis process of alveolar epithelial cells in APALI in the existing clinical application. The preparation method and application of lung matrix hydrogel comprises the following steps: crushing acellular lung tissues of a rat; then, adding into a hydrochloric acid-pepsin solution for dissolving; adding sodium hydroxide and PBS into the solution for adjusting to a physiological state; placing at 37 DEG C until the lung matrix hydrogel is formed; the lung matrix hydrogel prepared by the preparation method of the lung matrix hydrogel is applied to treatment of lung injury following severe acute pancreatitis; a medicine for treating the lung injury following severe acute pancreatitis comprises the lung matrix hydrogel prepared by the preparation method of the lung matrix hydrogel. When the lung matrix hydrogel prepared in the invention is used for preparing the medicine for treating the lung injury following severe acute pancreatitis, the medicine has an effect of adjusting and controlling the apoptosis process of alveolar epithelial cells in APALI, has low immunogenicity and substantially has no side effects.

Description

technical field [0001] The invention relates to the technical field of tissue engineering and regenerative medicine, in particular to a preparation method, application and medicine of a lung matrix hydrogel. Background technique [0002] Acute pancreatitis (AP) is one of the common acute abdomen diseases with high mortality rate. When AP occurs, pancreatic enzymes are abnormally activated in the acinar cells and release inflammatory mediators, thereby activating a large number of inflammatory cells, causing a "cascade reaction" of inflammation in the body, leading to multiple organ failure. Under the action of inflammatory mediators, the alveolar epithelial-endothelial cell barrier function is damaged, the circulating protein, liquid, neutrophils and other components enter the alveolar cavity, the alveolar structure is destroyed, the ventilation function is impaired, and pancreatitis-related lung cancer occurs. Injury (pancreatitis associated lung injury, APALI). APALI is ...

Claims

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Application Information

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IPC IPC(8): A61K35/42A61K9/06A61P1/18A61P11/00A61L27/52A61L27/36A61L27/50
Inventor 周菁孙红玉肖贞良田坤陈章连亨宁胡绳李志伟马李杰
Owner 中国人民解放军西部战区总医院
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