Method for synthesizing soybean oligosaccharides through biological catalysis

A technology of soybean oligosaccharide and synthase, applied in the direction of fermentation, can solve the problems of high price, difficulty and low yield of plant extraction method.
CN107586809AInactive Publication Date: 2018-01-16TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Publication Date
2018-01-16
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a method for synthesizing soybean oligosaccharides through biological catalysis. The method has the beneficial effects that that through establishing an in-vitro multi-enzyme cascade reaction system composed of sucrose synthetase, uridine diphosphate glucose 4-differential isomerase, inositol galactoside synthase, raffinose synthase and threonose synthase, sucrose is takenas a substrate to synthesize a single component or a mixed component in inositol galactoside, raffinose and threonose; the method realizes the recycling conversion of uridine diphosphate and inositol,and a crude enzyme solution added in the system contains a small amount of uridine diphosphate, so that the expensive uridine diphosphate can be avoided; the inositol galactoside yield reaches 50 g / L, the conversion rate is 70 percent, the raffinose yield reaches 20 g / L, the threonose yield reaches 10 g / L, and the obtained inositol galactoside, raffinose and threonose can be used in the fields offood, medicines and the like; the method disclosed by the invention is of great significance in the utilization of high attachment values of the sucrose and a biomass rich in the sucrose.
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Description

technical field

[0001] The invention relates to the field of biocatalysis, in particular to an in vitro multi-enzyme cascade catalyzed method for converting sucrose into soybean oligosaccharides. Background technique

[0002] Soy oligosaccharides are the general term for soluble sugars in soybeans. The main ingredients refer to sucrose (disaccharides), raffinose (trisaccharides) and stachyose (tetrasaccharides) with a monosaccharide number of 3 to 4. It is a sweetener with low sweetness and low calories. Replace sucrose in functional food or low-energy food; improve the balance of intestinal flora, prevent constipation; inhibit the production of harmful substances in the intestinal tract; reduce the concentration of serum cholesterol and blood lipids; enhance the body's immunity and inhibit the growth of tumor cells; Promote the generation and absorption of nutrients in the intestines and protect the liver. Soybean oligosaccharides are fermented or partially fermented in t...

Claims

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