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Preparation method of nano-fiber scaffold for promoting cardiac muscle cell reconstruction

A nanofiber, cardiomyocyte technology, applied in conductive/antistatic filament manufacturing, medical science, prosthesis, etc., can solve the problem that polydimethylsiloxane is difficult to manufacture nanofiber structure, poor biocompatibility, limitation Clinical application and other issues, to achieve the effect of improving cell compatibility, improving electrical conductivity, and good biocompatibility

Inactive Publication Date: 2018-01-19
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, polydimethylsiloxane is often used as a scaffold material to manufacture cardiomyocyte biological preparations, but polydimethylsiloxane has poor biocompatibility and is difficult to biodegrade. In addition, polydimethylsiloxane is difficult to manufacture The nanofibrous structure used to simulate the extracellular matrix of cardiomyocytes to promote the growth of cardiomyocytes has great limitations, which greatly limits its clinical application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Dissolving polyaniline (PANI) and camphorsulfonic acid (CSA) with a weight average molecular weight of 20,000 in hexafluoroisopropanol, stirring evenly to obtain a polyaniline mass percentage concentration of 1.5%, and camphorsulfonic acid mass percent A mixed solution with a concentration of 0.5%;

[0025] (2) adding polycaprolactone (PCL) with a weight average molecular weight of 100,000 to the mixed solution obtained in step (1), stirring evenly to obtain a spinning solution with a polymer concentration of 20 wt %;

[0026] (3) Input the spinning solution in step (2) to the spinneret of the electrospinning equipment at a propulsion speed of 15 μL / min, and connect the spinneret to a 21KV power supply for electrospinning to prepare a composite nanometer with a diameter of 600 nm. For fiber, collect electrospun nanofibers with a stainless steel drum with a rotating speed of 200rpm / min, and the receiving distance between the stainless steel drum and the spinneret is ...

Embodiment 2

[0029] (1) Dissolving polyaniline (PANI) and camphorsulfonic acid (CSA) with a weight average molecular weight of 10,000 in hexafluoroisopropanol, stirring evenly to obtain a polyaniline mass percentage concentration of 0.5%, and camphorsulfonic acid mass percent A mixed solution with a concentration of 0.2%;

[0030] (2) adding poly-L-lactide with a weight average molecular weight of 60,000 to the mixed solution obtained in step (1), stirring evenly to obtain a spinning solution with a polymer concentration of 10wt%;

[0031] (3) Input the spinning solution in step (2) to the spinneret of the electrospinning equipment at a propulsion speed of 20 μL / min, and at the same time connect the spinneret to a 17KV power supply for electrospinning to prepare a composite nanotube with a diameter of 450 nm. Fiber, collect the electrospun nanofiber with the stainless steel roller of rotating speed 300rpm / min, the receiving distance between stainless steel roller and spinneret is 21cm;

...

Embodiment 3

[0034] (1) Dissolving polyaniline (PANI) and camphorsulfonic acid (CSA) with a weight average molecular weight of 30,000 in hexafluoroisopropanol, stirring evenly to obtain a polyaniline mass percentage concentration of 4%, and camphorsulfonic acid mass percent The concentration is 0.8% mixed solution;

[0035] (2) Adding poly(glycolactide) with a weight average molecular weight of 200,000 to the mixed solution obtained in step (1), stirring evenly to obtain a spinning solution with a polymer concentration of 30wt%;

[0036] (3) Input the spinning solution in step (2) to the spinneret of the electrospinning equipment at a propulsion speed of 10 μL / min, and at the same time connect the spinneret to a 28KV power supply for electrospinning to prepare a composite nanotube with a diameter of 900 nm. For fibers, collect electrospun nanofibers with a stainless steel drum with a rotating speed of 100rpm / min, and the receiving distance between the stainless steel drum and the spinneret...

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PUM

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Abstract

The invention belongs to the field of preparation of nanometer functional materials. At present, a cardiac muscle cell biological agent is prepared by often using polydimethylsiloxane as a scaffold material. However, the polydimethylsiloxane is poor in biocompatibility and difficult to be biodegraded, is difficult to manufacture a nano-fiber structure and has a great limitation for simulating a cardiac muscle cell extracellular matrix to promote growth of the cardiac muscle cells. Aiming at the problems existing in the prior art, the invention discloses a preparation method of a nano-fiber scaffold for promoting cardiac muscle cell reconstruction. The nano-fiber scaffold with electroactivity is prepared by performing mixed electrostatic spinning on polyaniline and an appropriate. The preparation method disclosed by the invention is simple, and the prepared nano-fiber scaffold has biocompatibility and electroactivity and can promote the growth of the cardiac muscle cells.

Description

technical field [0001] The invention relates to a preparation method of a nanofiber support for promoting cardiomyocyte reconstruction, and belongs to the field of preparation of nano functional materials. Background technique [0002] At present, 17.5 million people die from heart disease and vascular disease every year in the world, accounting for 30% of the total number of deaths from diseases. Among them, myocardial infarction is one of the main causes of death. Due to factors such as the low regeneration capacity of cardiomyocytes and the formation of scar tissue around the infarction site, it is difficult to restore damaged myocardial tissue. The use of tissue engineering technology to construct cardiomyocyte-based biological agents can overcome the above shortcomings and help cardiomyocytes recover and regenerate. [0003] At present, polydimethylsiloxane is often used as a scaffold material to manufacture cardiomyocyte biological preparations, but polydimethylsiloxan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F6/92D01F1/09A61L27/18A61L27/50A61L27/58
Inventor 赵亮亮许杉杉孟庆怡
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS