Special fertilizer for disease resistance of bergamot yams and preparation method of fertilizer
A special fertilizer, yam technology, applied in the application, nitrogen fertilizer, potash fertilizer and other directions, can solve the problems affecting the quality and yield of yam, farmers' economic losses, etc., and achieve the effects of low cost, reduced incidence, and simple preparation method.
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Embodiment 1
[0028] Embodiment 1: Antagonism test of different bacterial strains to anthracnose pathogenic bacteria
[0029] Inoculate the pathogenic bacteria Colletotrichum gloeosporioides in the center of the PDA medium plate and culture at 25°C. In the well-grown plate of Colletotrichum gloeosporioides, use a 1 cm diameter puncher to cut out colony circles at the edge of the colony for future use. Dilute the Sphingomonas liquid and the Brevibacillus potsdam liquid in the logarithmic growth phase with sterile water to a cell concentration of 1×10 5 pieces / ml. Add 100 μL of the test bacteria dilution to the sterilized PDA plate, spread it evenly with a spreader, and repeat 4 times for each treatment. The compound bacterial liquid treatment group was formed by mixing Sphingomonas and Brevibacillus Potsdam at a ratio of 1:1. Inoculate the cut gliospora anthrax bacteria block (diameter 1cm) in the center of the coated plate with the mycelium facing down, and after incubating upside down at...
Embodiment 2
[0035] Example 2: Production of special fertilizer for bergamot yam disease resistance
[0036](1) Heap the manure of livestock and poultry to a height of 1.0-1.2 meters, turn the pile when the temperature of the pile reaches 80°C, and add water to keep the water content of the compost within the range of 30-32%. matrix;
[0037] (2) Flatten the solid fermentation substrate and make it drop to normal temperature, then insert respectively the Sphingomonas fermentation liquid and the Brevibacillus potsdam fermentation liquid in the logarithmic growth phase according to the standard of 25L per ton of substrate, stir and mix, Then the pile height is 0.4-0.5 meters, the pile is turned when the pile temperature reaches 35° C., spread out after continuous fermentation for 6 days, and the water content of the fertilizer is evaporated to below 30% to obtain bio-organic fertilizer. The number of effective viable bacteria per gram is not less than 0.4 billion, the organic matter content...
Embodiment 3
[0039] Example 3: Field test of bergamot yam disease-resistant special fertilizer for disease-resistant and yield-increasing
[0040] On April 10, 2016, a field fertilizer test was carried out. Before planting, fertilize in the soil ditch according to the fertilization method of each treatment, make a furrow after filling the soil, and open a small ditch on the furrow for planting, with a row spacing of 30 cm, a depth of about 10 cm, and a plant spacing of about 15 cm. The soil should be leveled to avoid uneven watering. When planting, the bergamot yam seeds should be placed in the same direction, and then covered with soil. In summer, water and fertilizer management should be strengthened, and drainage design should be done well to ensure that there is no water in the fields, so as to reduce the occurrence and spread of diseases and insect pests. Wuxue bergamot yam is harvested after Lidong. Except for the amount of fertilization and its types, the other management measure...
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