Ludisia discolor tissue culture method

Abstract

The invention discloses a ludisia discolor tissue culture method. The ludisia discolor tissue culture method comprises four steps of culturing and germinating ludisia discolor seeds into round bulbs,culturing strong seedlings, inducing rooting and hardening the seedlings, wherein a seed culture medium used in the step of culturing and germinating the ludisia discolor seeds into the round bulbs isprepared from the following components: 1,600mg / L of potassium nitrate, 1,250mg / L of ammonium nitrate, 370mg / L of magnesium sulfate, 170mg / L of monopotassium phosphate, 440mg / L of calcium chloride, 0.025mg / L of copper sulfate, 0.025mg / L of cobalt chloride, 16.9mg / L of manganese sulfate, 8.6mg / L of zinc sulfate, 6.2mg / L of boric acid, 0.83mg / L of potassium iodide, 0.25mg / L of sodium molybdate, 37.3mg / L of Na2-EDTA (Ethylene Diamine Tetraacetate), 27.8mg / L of ferrous sulfate, 2.0mg / L of glycine, 0.1mg / L of thiamine hydrochloride, 0.5mg / L of pyridoxine hydrochloride, 0.5mg / L of nicotinic acid, 100mg / L of inositol, 1 to 10 mg / L of NAA (Naphthalene Acetic Acid), 5 to 10 mg / L of 6-BA (Butyl Acetate), 100,000mg / L of potatoes, 250mg / L of carbon powder, 30,000mg / L of saccharose and 700mg / L of lactoalbumin hydrolysate. According to the ludisia discolor tissue culture method disclosed by the invention, the germination rate of the ludisia discolor seeds is high and is 90 percent or more, and thereproductive rate is greatly increased.

Description

technical field [0001] The invention relates to a method for culturing blood leaf orchid tissue. Background technique [0002] Blood leaf orchid (Ludisia discolor (Ker-Gawl.) A. Rich.): Plant height 10-25 cm. Rhizome elongated, prostrate, articulate. Stem erect, with 3-4 leaves near base. Leaves are ovate or ovate-oblong, black-green above, with 5 golden-red shiny veins, and light red on the back; 2-3 light red sheath-like bracts on the stem above the leaves. Racemes terminal, with several to more than 10 flowers; flowers white or reddish, about 7mm in diameter; petals nearly semiovate, 8-9mm long, 2-2.2mm wide, apex obtuse. The flowering period is from February to April. At present, the propagation of blood leaf orchid mainly depends on branch propagation or cutting propagation, which can be carried out in spring and autumn, and the branch propagation is generally carried out every three years. However, the propagation efficiency of the above-mentioned propagation meth...

AI Technical Summary

Problems solved by technology

However, the propagation efficiency of the above-mentioned propagation method is low and cannot meet the market demand. Therefore, it is necessary to use plant tissue culture technology to rapidly propagate the blood leaf orchid.

However, the germination rate of the existing blood leaf orchid tissue culture is low, and the productive rate is not high

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