Ludisia discolor tissue culture method

A technology of tissue culture and blood leaf orchid, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of inability to meet market demands, low reproductive efficiency, low germination rate, etc. , the effect of high germination rate

Pending Publication Date: 2018-02-23
容县明曦铁皮石斛种植场(微型企业)
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the propagation efficiency of the above-mentioned propagation method is low and cannot meet the market demand. Therefore, it is necessary to use plant tissue culture

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ludisia discolor tissue culture method
  • Ludisia discolor tissue culture method
  • Ludisia discolor tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Blood leaf orchid tissue culture is divided into four stages, is respectively four stages of seed germination, strong seedling cultivation, induced rooting and seedling hardening, so the blood leaf orchid tissue culture method provided in the present invention comprises blood leaf orchid seed germination and becomes a round bulb, Strong seedlings, rooting, hardening four steps, the specific cultivation process is as follows:

[0025] (1) Seeds germinate into round bulbs: take mature blood leaf orchid seeds for disinfection treatment, rinse with tap water and detergent for 3 minutes, then sterilize with 75% alcohol for 30 seconds, then sterilize with 35% hydrogen peroxide for 10 minutes, and finally use aseptic Rinse with water three times and sow in the seed medium for cultivation, turn green in one week, continue to cultivate for 20-30 days, after the seed embryo expands and germinates to form a green round bulb, the germination rate of the seeds is above 90%. The cult...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a ludisia discolor tissue culture method. The ludisia discolor tissue culture method comprises four steps of culturing and germinating ludisia discolor seeds into round bulbs,culturing strong seedlings, inducing rooting and hardening the seedlings, wherein a seed culture medium used in the step of culturing and germinating the ludisia discolor seeds into the round bulbs isprepared from the following components: 1,600mg/L of potassium nitrate, 1,250mg/L of ammonium nitrate, 370mg/L of magnesium sulfate, 170mg/L of monopotassium phosphate, 440mg/L of calcium chloride, 0.025mg/L of copper sulfate, 0.025mg/L of cobalt chloride, 16.9mg/L of manganese sulfate, 8.6mg/L of zinc sulfate, 6.2mg/L of boric acid, 0.83mg/L of potassium iodide, 0.25mg/L of sodium molybdate, 37.3mg/L of Na2-EDTA (Ethylene Diamine Tetraacetate), 27.8mg/L of ferrous sulfate, 2.0mg/L of glycine, 0.1mg/L of thiamine hydrochloride, 0.5mg/L of pyridoxine hydrochloride, 0.5mg/L of nicotinic acid, 100mg/L of inositol, 1 to 10 mg/L of NAA (Naphthalene Acetic Acid), 5 to 10 mg/L of 6-BA (Butyl Acetate), 100,000mg/L of potatoes, 250mg/L of carbon powder, 30,000mg/L of saccharose and 700mg/L of lactoalbumin hydrolysate. According to the ludisia discolor tissue culture method disclosed by the invention, the germination rate of the ludisia discolor seeds is high and is 90 percent or more, and thereproductive rate is greatly increased.

Description

technical field [0001] The invention relates to a method for culturing blood leaf orchid tissue. Background technique [0002] Blood leaf orchid (Ludisia discolor (Ker-Gawl.) A. Rich.): Plant height 10-25 cm. Rhizome elongated, prostrate, articulate. Stem erect, with 3-4 leaves near base. Leaves are ovate or ovate-oblong, black-green above, with 5 golden-red shiny veins, and light red on the back; 2-3 light red sheath-like bracts on the stem above the leaves. Racemes terminal, with several to more than 10 flowers; flowers white or reddish, about 7mm in diameter; petals nearly semiovate, 8-9mm long, 2-2.2mm wide, apex obtuse. The flowering period is from February to April. At present, the propagation of blood leaf orchid mainly depends on branch propagation or cutting propagation, which can be carried out in spring and autumn, and the branch propagation is generally carried out every three years. However, the propagation efficiency of the above-mentioned propagation meth...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 涂湘炎
Owner 容县明曦铁皮石斛种植场(微型企业)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap