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PLGA/zwitterionic composite porous bone tissue engineering scaffold and preparation method thereof

A tissue engineering scaffold, zwitterion technology, applied in tissue regeneration, pharmaceutical formulations, coatings, etc., can solve the problems of unstable degradation rate, lack of efficient slow release performance of growth factors, tissue inflammatory response, etc., to improve the biological phase. Capacitive and biosafety properties, stable degradation, and simple preparation methods

Active Publication Date: 2018-02-23
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure PLGA materials have some disadvantages as scaffolds for bone tissue processes: the degradation rate is not stable, and there is a rapid degradation phenomenon, and the local high concentration of acid caused by the rapid degradation phenomenon is likely to cause tissue inflammation; releasing performance

Method used

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  • PLGA/zwitterionic composite porous bone tissue engineering scaffold and preparation method thereof
  • PLGA/zwitterionic composite porous bone tissue engineering scaffold and preparation method thereof
  • PLGA/zwitterionic composite porous bone tissue engineering scaffold and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0027] The preparation method of the PLGA / zwitterionic composite porous bone tissue engineering scaffold is as follows:

[0028] (1) Preparation of PLGA polymer

[0029] With 0.43g of polyethylene glycol (PEG) with a molecular weight of 700 as the initiator, and 100 μL of stannous isooctanoate as a catalyst with a concentration of 8% w / v, pass through 0.1g of lactide and 9.9g of glycolide at a temperature of 200 ° C. After reacting for 24 hours in methanol, it was settled in methanol and then dried under vacuum to obtain PLGA polymer.

[0030] (2) Preparation of porous PLGA scaffolds

[0031] The PLGA polymer prepared in step (1) is dissolved in 1,4-dioxane to form a solution with a concentration ratio of 2 wt%, and after dissolving, it is injected into a polytetrafluoroethylene cake mold and frozen at -20°C After 4 hours, it was freeze-dried at -50° C. for 12 hours under vacuum conditions to obtain a porous PLGA scaffold with a pore size of about 300 μm.

[0032] (3) Plasm...

Embodiment 2

[0038] The preparation method of the PLGA / zwitterionic composite porous bone tissue engineering scaffold is as follows:

[0039] (1) Preparation of PLGA polymer

[0040] Using 0.3g of PEG with a molecular weight of 1000 as the initiator, and 100 μL of stannous octoate with a concentration of 8% w / v as the catalyst, react 7.5g of lactide and 2.5g of glycolide at a temperature of 140°C for 24h, and pass through methanol After settling in medium, dry under vacuum condition to obtain PLGA polymer.

[0041] (2) Preparation of porous PLGA scaffolds

[0042]Dissolving the PLGA polymer prepared in step (1) in 1,4-dioxane to form a solution with a concentration ratio of 10 wt%, inject it into a polytetrafluoroethylene cake mold after dissolving, and freeze at -60°C After 12 hours, it was freeze-dried at -60° C. for 36 hours under vacuum conditions to obtain a porous PLGA scaffold with a pore size of about 200 μm.

[0043] (3) Plasma surface treatment of porous PLGA scaffolds

[004...

Embodiment 3

[0049] The preparation method of the PLGA / zwitterionic composite porous bone tissue engineering scaffold is as follows:

[0050] (1) Preparation of PLGA polymer

[0051] Using 0.15g of PEG with a molecular weight of 2000 as the initiator, and 100 μL of stannous octoate with a concentration of 8% w / v as the catalyst, react 9.9g of lactide and 0.1g of glycolide at a temperature of 100°C for 72h, and pass through methanol After settling in medium, dry under vacuum condition to obtain PLGA polymer.

[0052] (2) Preparation of porous PLGA scaffolds

[0053] Dissolving the PLGA polymer prepared in step (1) in 1,4-dioxane to form a solution with a concentration ratio of 20 wt%, inject it into a polytetrafluoroethylene cake mold after dissolving, and freeze at -80°C After 36 hours, it was freeze-dried at -80° C. for 96 hours under vacuum conditions to obtain a porous PLGA scaffold with a pore size of about 20 μm.

[0054] (3) Plasma surface treatment of porous PLGA scaffolds

[00...

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Abstract

The invention provides a composite porous bone tissue engineering scaffold. The composite porous bone tissue engineering scaffold comprises a porous PLGA scaffold and zwitterionic hydrogel compoundedwith the porous PLGA scaffold. The invention also provides a method for preparing the composite porous bone tissue engineering scaffold. The method comprises preparing a zwitterionic hydrogel performed liquid containing a zwitterionic monomer, an initiator and a crosslinking agent, immersing the porous PLGA scaffold subjected to plasma surface modification in the performed liquid, taking out the porous PLGA scaffold and carrying out UV crosslinking and freeze drying. Through compounding of the zwitterions and the porous PLGA scaffold, the sharp degradation of the common biodegradable tissue engineering scaffold is avoided, stable degradation of the whole scaffold is realized and the biocompatibility and biosafety of the scaffold material are greatly improved. Through compounding of the zwitterionic hydrogel and the PLGA scaffold, the whole scaffold has a growth factor efficient sustained-release function.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a PLGA / zwitterion composite porous bone tissue engineering scaffold and a preparation method thereof. Background technique [0002] With the aging of the global population, the repair of bone defects has become an increasingly serious clinical and socioeconomic problem facing mankind. Autologous bone (auto-grafts) has a good physical and physiological similarity with the host bone, can effectively integrate with the host bone and promote the repair of bone defects, and is the "gold standard" for clinical bone defect repair at this stage. However, the source of autologous bone is very limited and prone to complications, while allo-grafts have a wide range of sources, but there are risks of cross-infection, disease transmission, and rejection. Therefore, it is of great scientific and socioeconomic significance to develop effective alternative bone repair materials. [0003] Ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/34A61L27/18A61L27/56A61L27/58A61L27/52
CPCA61L27/18A61L27/34A61L27/52A61L27/56A61L27/58A61L2400/18A61L2430/02C08L67/04C08L51/08
Inventor 刘平生沈健刘沛铭
Owner NANJING NORMAL UNIVERSITY
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