Gradient preparation method of ultrahigh barley malt syrup
A maltose syrup and gradient technology, which is applied in the field of gradient preparation of ultra-high maltose syrup, can solve the problems of environmental pollution and high production cost, and achieve the effects of simple and easy process, improved utilization rate, and increased concentration.
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Embodiment 1
[0034] A gradient preparation method for ultra-high maltose syrup, characterized in that: the gradient preparation method follows the steps:
[0035] Step 1, adding starch into an aqueous ethanol solution, stirring and dissolving by ultrasonic to obtain a starch solution;
[0036] Step 2, the starch solution is sealed and pressurized for 1 hour, left to stand for 10 minutes, and filtered after rapid pressure release to obtain a refined starch solution;
[0037] Step 3, add pullulanase and β-amylase to citric acid-sodium citrate buffer, stir well to obtain the first enzymolysis solution; add pullulanase and maltase to citric acid-citric acid In sodium buffer solution, after stirring evenly, the second enzymolysis solution was obtained
[0038] Step 4, adding the first enzymolysis liquid to the inner layer reaction kettle in the double-layer reaction kettle, and microwave heating to a constant temperature; adding the second enzymolysis liquid to the outer layer reaction kettle,...
Embodiment 2
[0052] A gradient preparation method for ultra-high maltose syrup, characterized in that: the gradient preparation method follows the steps:
[0053] Step 1, adding starch into an aqueous ethanol solution, stirring and dissolving by ultrasonic to obtain a starch solution;
[0054] Step 2, the starch solution is sealed and pressurized for 2 hours, left to stand for 30 minutes, and filtered after rapid pressure release to obtain a refined starch solution;
[0055] Step 3, add pullulanase and β-amylase to citric acid-sodium citrate buffer, stir well to obtain the first enzymolysis solution; add pullulanase and maltase to citric acid-citric acid In sodium buffer solution, after stirring evenly, the second enzymolysis solution was obtained
[0056] Step 4, adding the first enzymolysis liquid to the inner layer reaction kettle in the double-layer reaction kettle, and microwave heating to a constant temperature; adding the second enzymolysis liquid to the outer layer reaction kettle...
Embodiment 3
[0070] A gradient preparation method for ultra-high maltose syrup, characterized in that: the gradient preparation method follows the steps:
[0071] Step 1, adding starch into an aqueous ethanol solution, stirring and dissolving by ultrasonic to obtain a starch solution;
[0072] Step 2, the starch solution is sealed and pressurized for 2 hours, left to stand for 20 minutes, and filtered after rapid pressure release to obtain a refined starch solution;
[0073] Step 3, add pullulanase and β-amylase to citric acid-sodium citrate buffer, stir well to obtain the first enzymolysis solution; add pullulanase and maltase to citric acid-citric acid In sodium buffer solution, after stirring evenly, the second enzymolysis solution was obtained
[0074] Step 4, adding the first enzymolysis liquid to the inner layer reaction kettle in the double-layer reaction kettle, and microwave heating to a constant temperature; adding the second enzymolysis liquid to the outer layer reaction kettle...
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