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Method for separating L-glutamic acid from electroosmosis fresh water

A technology of glutamic acid and electrodialysis, which is applied in the field of bioengineering, can solve the problems of industrialized production and environmental protection management, the harsh requirements of chemical synthesis process conditions, and the proneness to side reactions, etc., achieving outstanding implementation effects and specific substrate Strong performance and high enzymatic catalytic efficiency

Inactive Publication Date: 2018-03-06
湖北新生源生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process conditions of the chemical synthesis method are demanding, side reactions are prone to occur, industrial production and environmental protection are difficult, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Cultivation of strains: Culture Pseudomonas de akunha in 1000 mL medium at 37°C for 15 hours with shaking, and centrifuge at 4000r / min for 15 min to obtain wet bacteria with aspartate-β-decarboxylase activity. Cells 15 g, medium formula (g / mL): 4.0% corn steep liquor, 0.5% protein hydrolyzate, 0.5% beef extract, 1.0% maltose, 0.5% lactose, 0.5% NaCl, KH 2 PO 4 0.5%, MgSO 4 •7H 2 O 0.05%, the pH value of the medium was controlled at 7.2.

[0026] 2. Carry out enzymatic reaction: take the fresh water effluent after the keratin hydrolyzate is treated by the electrodialysis device, after concentration and desalination, adjust the pH value to 5.0 with hydrochloric acid with a mass percentage concentration of 30%, and set the volume to 1000 mL, where L - The total concentration of glutamic acid and L-aspartic acid is 50 g / L, the mass ratio of L-glutamic acid to L-aspartic acid is 20:80, and the addition of aspartic acid-β-decarboxylase activity 15 g of wet bacteria, an...

Embodiment 2

[0029] 1. Cultivation of strains: Culture Pseudomonas deaquinha in 1000 mL culture medium at 37°C for 14 hours with shaking, and centrifuge at 4000r / min for 15 minutes to obtain wet bacteria with aspartate-β-decarboxylase activity. Cells 13 g, medium formula (g / mL): corn steep liquor 1.0%, NaCl 0.5%, KH 2 PO 4 0.05%, MgSO 4 •7H2 O 0.03%, beef extract 2%, glucose 1%, maltose 0.5%, the pH value in the medium is controlled at 7.2.

[0030] 2. Carry out enzymatic reaction: take the fresh water effluent after the keratin hydrolyzate is treated by the electrodialysis device, after concentration and desalination, adjust the pH value to 6.0 with hydrochloric acid with a mass percentage concentration of 30%, and set the volume to 1000 mL, where L - The total concentration of glutamic acid and L-aspartic acid is 100 g / L, the mass ratio of L-glutamic acid and L-aspartic acid is 30:70, and the addition of aspartic acid-β-decarboxylase activity 13 g of wet bacteria, and Tween 80 with a...

Embodiment 3

[0033] 1. Cultivation of strains: Culture Pseudomonas de akunha in 1000 mL medium at 37°C for 15 hours with shaking, and centrifuge at 4000r / min for 15 min to obtain wet bacteria with aspartate-β-decarboxylase activity. Cells 14 g, medium formula (g / mL): corn steep liquor 1.0%, protein hydrolyzate 0.5%, beef extract 2%, maltose 1.0%, lactose 0.5%, NaCl 0.5%, KH 2 PO 4 0.5%, MgSO 4 •7H 2 O 0.04%, the pH value of the medium was controlled at 7.2.

[0034] 2. Carry out enzymatic reaction: take the fresh water effluent after the keratin hydrolyzate is treated by the electrodialysis device, after concentration and desalination, adjust the pH value to 5.0 with hydrochloric acid with a mass percentage concentration of 30%, and set the volume to 1000 mL, where L - The total concentration of glutamic acid and L-aspartic acid is 100 g / L, the mass ratio of L-glutamic acid and L-aspartic acid is 40:60, and the addition of aspartic acid-β-decarboxylase activity 14 g of wet bacteria, t...

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Abstract

The invention provides a method for separating L-glutamic acid from electroosmosis fresh water. The method comprises the following steps: (1) culturing a strain; (2) carrying out enzymatic reaction; and (3) preparing L-glutamic acid. According to the method, two acidic amino acids with similar properties are separated by virtue of an enzyme method, the specifity of a substrate is strong, reactionconditions are mild, and the problem of efficient separation of two mixed acidic amino acids is solved; and cheap biological resources are adequately utilized in enzyme method conversion and separation processes, so that the method has the advantages that the raw material sources are wide, the production cost is low, the implementation effect is outstanding, and the economic and social benefits are good.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for separating L-glutamic acid from electrodialysis fresh water. Background technique [0002] L-glutamic acid is widely used in the food industry, for example as a raw material in the production of flavoring agents. At present, it is usually obtained by fermentation of coryneform bacteria capable of producing L-glutamic acid, and large-scale industrial production has been realized. [0003] L-alanine is a non-essential amino acid in the human body. In the pharmaceutical industry, L-alanine is one of the main components of many compound amino acid injections; in the food industry, L-alanine is used as a nutrient Adjuncts and artificial sweeteners can not only improve the utilization of protein in food and beverages, but also improve the taste of many artificial sweeteners and increase the sweetness. At present, the main methods of L-alanine production include enzymatic co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C12P13/20C12P13/06C12R1/15C12R1/38
CPCC12P13/06C12P13/14C12P13/20
Inventor 吴聪李娟曾庆国袁劲松刘均忠焦庆才章平肖国安胡家艳
Owner 湖北新生源生物工程有限公司
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