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Micro-ecological regulator for necrotizing enterocolitis of piglets, preparation method thereof and feed containing regulator

A technology of microecological regulator and necrotic enteritis, which is applied in the field of veterinary biological products manufacturing, can solve the problems of poor vaccine protection, acute pathogenicity, and short toxin production time, and achieve the goal of preventing intestinal diseases and improving nutritional value Effect

Inactive Publication Date: 2018-03-13
JIANGXI AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional feeding method in the past, piglets were born with the habit of arching the sow's feces after birth, but under intensive breeding, the way of manure treatment makes it difficult for the piglets after birth to come into contact with the sow's feces
[0005] At present, the method used to prevent NEC in piglets refers to the immunization method of sheep rapid disease. After immunizing pregnant sows with vaccines, piglets are protected through breast milk, but Cp produces a variety of toxins, and the protection of vaccines is poor; and the toxins are produced due to individual differences in piglets. However, the time of toxin production is short, the disease is acute, and drug treatment is usually difficult to control. Therefore, there are certain limitations in the prevention of this disease.

Method used

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  • Micro-ecological regulator for necrotizing enterocolitis of piglets, preparation method thereof and feed containing regulator
  • Micro-ecological regulator for necrotizing enterocolitis of piglets, preparation method thereof and feed containing regulator
  • Micro-ecological regulator for necrotizing enterocolitis of piglets, preparation method thereof and feed containing regulator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] LB liquid medium: Weigh 10g of tryptone, 5g of yeast extract and 10g of NaCl in a beaker, add 800mL of distilled water into the beaker and stir with a glass rod to dissolve all the medicines, adjust the pH to 7.2 with 1M NaOH, pour the solution Add distilled water to the measuring cylinder to 1L, sterilize with high-pressure steam for 20 minutes, add the required antibiotics on the ultra-clean bench and shake well when the temperature of the culture medium drops to 50°C, and spread about 20mL of culture medium in a 90mm diameter petri dish, 4°C save.

[0068] The preparation method of piglet necrotic enteritis microecological regulator is implemented according to the following steps:

[0069] (1) Expanded culture of strains: Lactobacillus salivarius CGMCC No.14458 strains were inoculated in MRS liquid medium with an inoculum size of 8%, and cultured at a constant temperature for 12 hours at 35°C; then Bacillus subtilis GIM 1.976 Inoculate into LB liquid medium with an ...

Embodiment 2

[0073] The preparation method of piglet necrotic enteritis microecological regulator is implemented according to the following steps:

[0074] (1) Expanded culture of strains: Lactobacillus salivarius CGMCC No.14458 was inoculated in MRS liquid medium with an inoculum size of 8%, and cultured at a constant temperature for 12 hours at 37°C; then Bacillus subtilis GIM 1.976 was inoculated into LB In the liquid culture medium, the inoculum size is 16%. At 37°C, culture at constant temperature for 12 hours, supplement carbon source maltose 6g / 100mL, nitrogen source peptone 6g / 100mL and 0.3g / 100mL inorganic salt magnesium chloride and disodium hydrogen phosphate, Continue to ferment and cultivate for 60 hours, mix the two bacterial liquids, and obtain the mixed bacterial fermentation liquid;

[0075] (2) Obtaining of microbial fermentation broth: adjusting the pH value of the mixed bacteria fermentation broth obtained in step (1) to 4.0 to obtain a liquid microbial fermentation bro...

Embodiment 3

[0078] Soybean meal feed preparation method:

[0079] In cooperation with Jiangxi Nanchang Interui Biological Co., Ltd., culture and ferment the microbial fermentation liquid prepared in the above-mentioned Example 1 to the highest peak stage of the logarithmic growth phase, and inoculate it in unfermented soybean meal according to the mass ratio of 1:10. Fermentation, after 72 hours of fermentation, the obtained fermented soybean meal was added to piglet feed with a mass ratio of 1:50.

[0080] By observing and testing the crude protein, crude fiber, particle size and other data of soybean meal before and after fermentation, as well as the comparison between fermented soybean meal and feed raw material fish meal, the data shown in Table 4 and Table 5 below are obtained:

[0081] Table 4 Comparison of soybean meal before and after fermentation by Lactobacillus salivarius

[0082]

[0083] Table 5 Comparison of fermented soybean meal and feed raw fish meal

[0084]

[0...

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Abstract

The present invention discloses a micro-ecological regulator for necrotizing enterocolitis (NEC) of piglets, a preparation method thereof and a feed containing the regulator, and belongs to the fieldof veterinary biological product manufacturing. The regulator is a microbial fermentation solution comprising two strains of lactobacillus salivarius CGMCC No.14458 and bacillus subtilis GIM 1.976. Apure strain expanded culture and a mixed strain liquid fermentation culture are conducted, finally the microbial fermentation solution with viable organisms in dormant states is obtained, and a totalbacterial count of the microbial strains is larger than 4.0x10<9> cfu / mL. The disclosed micro-ecological regulator for the necrotizing enterocolitis of the piglets can help the piglets to establish amicro-ecological flora, and forms an intestinal lactic acid environment beneficial for preventing intestinal diseases, particularly NEC, of the piglets within 7 days of age. The disclosed feed containing the regulator can replace antibiotics to prevent enteritis and other diseases of the piglets.

Description

technical field [0001] The invention relates to the field of veterinary biological product manufacturing, and more specifically relates to a microecological regulator for piglet necrotic enteritis, a preparation method thereof, and a feed containing the regulator. Background technique [0002] Piglet diarrhea is an important intestinal disease that harms piglets and has caused great losses to the pig industry. There are many causes of diarrhea in piglets, such as antibiotic-related diarrhea, virus-related diarrhea, and stress-induced diarrhea. Excessive use of antibiotics can cause intestinal flora disorder, reduce normal flora, increase opportunistic pathogens, and further develop diarrhea. Necrotizing enterocolitis (NEC) is a new syndrome of neonatal diarrhea in piglets, mainly caused by toxins produced by Clostridium perfringens (Cp) type A. Cp is an opportunistic pathogenic bacterium. It is easy to grow in large quantities in the intestinal tract and produce toxins to ...

Claims

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Application Information

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IPC IPC(8): A23K50/30A23K10/37A23K10/12C12N1/20C12R1/225C12R1/125
CPCA23K10/12A23K10/37A23K50/30C12N1/20A23V2400/181Y02P60/87
Inventor 张锦华刘宝生张庆生周姣李昆韩春生
Owner JIANGXI AGRICULTURAL UNIVERSITY
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