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Use of tanshinone in resisting uric acid nephropathy

A technology of uric acid nephropathy and tanshinone, applied in the field of natural medicines, can solve problems such as unclear structure of active ingredients, uncertain stability and reproducibility of quality control pharmacological experiment results, unclear synergistic effect, etc., and achieve good kidney protection , reduce blood uric acid, good curative effect

Inactive Publication Date: 2018-04-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the fat-soluble components of Danshen have anti-inflammatory and anti-oxidative effects, and the structures of tanshinone components are similar. It is not clear whether other components have a synergistic effect on tanshinone II A in kidney protection. The quality control of drugs, the stability and reproducibility of pharmacological experiment results will bring uncertain factors

Method used

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  • Use of tanshinone in resisting uric acid nephropathy
  • Use of tanshinone in resisting uric acid nephropathy
  • Use of tanshinone in resisting uric acid nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of tanshinone compounds and quantification of the four main components of tanshinone II A, 15,16-dihydrotanshinone I, cryptotanshinone and tanshinone.

[0033] 1 Materials and Instruments

[0034] The extraction and separation reagent ethyl acetate (Nanjing Chemical Reagents) was analytically pure, and the identification reagents methanol (Shanghai Xingkegao) and acetonitrile (Merck) were chromatographically pure. Salvia miltiorrhiza was purchased from Shandong Rizhao.

[0035] Shimadzu liquid chromatography (Shimadzu Corporation), rotary evaporator EYELA OSB-2100 (Nanjing Huiheng).

[0036] 2 methods

[0037] 2.1 Extraction and separation

[0038] Weigh 150g of Salvia miltiorrhiza powder, put it into a stoppered Erlenmeyer flask, add 8 times the amount of ethyl acetate for ultrasonic extraction for 2 hours. The extracted obtained filtrate and filter residue were obtained by suction filtration, the filtrates were combined, concentrated by rotar...

Embodiment 2

[0062] Example 2 Detection of general indicators and biochemical indicators of the effects of tanshinone compounds and tanshinone II A monomers on mice with uric acid nephropathy.

[0063] 1 animal

[0064] SPF-grade Kunming mice were purchased from the Experimental Animal Center of the Academy of Military Medical Sciences of the Chinese People’s Liberation Army. After a week of adaptive feeding in an environment with a temperature of 22±3°C and a humidity of 65±5%, the experiment began. The mice had free access to food and water. .

[0065] 2 Materials and Instruments

[0066] Uric acid, creatinine, urea nitrogen test kit (Nanjing Jiancheng Bioengineering Research Institute).

[0067] Microplate reader (Beijing Hao Nuosi Technology Co., Ltd., Beijing Hao Nuosi Technology Co., Ltd., varioskanflash), electric constant temperature water bath (Guohua Electric Co., Ltd., HH-4 digital display constant temperature water bath), centrifuge (Thermo Corporation of the United States ,...

Embodiment 3

[0108] The preferred process of embodiment 3 four kinds of compound ratios in described composition and effect thereof

[0109] The ratio of the four compounds of tanshinone II A, 15,16-dihydrotanshinone I, cryptotanshinone and tanshinone in the composition is 6:3:6:4.

[0110] 1 animal

[0111] SPF-grade Kunming mice were purchased from the Experimental Animal Center of the Academy of Military Medical Sciences of the Chinese People’s Liberation Army. After a week of adaptive feeding in an environment with a temperature of 22±3°C and a humidity of 65±5%, the experiment began. The mice had free access to food and water. .

[0112] 2 Materials and Instruments

[0113] Tanshinone II A, 15,16-dihydrotanshinone I, cryptotanshinone, and tanshinone monomer compounds are all purchased from outside.

[0114] Uric acid, creatinine, urea nitrogen test kit (Nanjing Jiancheng Bioengineering Research Institute).

[0115] Microplate reader (Beijing Hao Nuosi Technology Co., Ltd., Beijing...

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Abstract

The invention relates to the field of natural medicine, in particular to a use of ethyl acetate extract of Salvia miltiorrhiza, namely tanshinone, in the preparation of drugs to resist uric acid nephropathy, and a use thereof in the preparation of antioxidant drugs. The drugs containing tanshinone are applied to treat uric acid nephropathy, particularly to prevent or treat hyperuricemia, gout andhyperuricemia-induced chronic nephropathy. Tanshinone mainly includes diterpene phenanthraquinone ingredients such as tanshinone IIA, 15,16-dihydrotanshinone I, cryptotanshinone and miltirone; owing to their synergy, the ingredients have better therapeutic effect in terms of prevention and treatment of uric acid nephropathy than single tanshinone IIA and the clinically common drug allopurinol under single dosage. When the four ingredients, tanshinone IIA, 15,16-dihydrotanshinone I, cryptotanshinone and miltirone, are combined in the optimal ratio of 6:3:6:4 into a tanshinone mixture, best prevention and treatment effect is provided for mouse uric acid nephropathy; through the use of the tanshinone combinations, Kunming mice co-modeled with adenine and oteracil potassium are significantly lower than model groups in terms of blood uric acid, creatinine and urea nitrogen, and kidney protection is achieved.

Description

technical field [0001] The invention relates to the field of natural medicines, in particular to the use of tanshinone compounds for preventing and treating uric acid nephropathy. Background technique [0002] Uric acid nephropathy (UAN), also known as gouty nephropathy, is due to purine metabolism disorder, excessive blood uric acid production or decreased renal uric acid excretion, resulting in elevated blood uric acid levels and deposition of urate crystals in the renal marrow. Renal damage due to interstitium. Clinical manifestations include uric acid stones, proteinuria, edema, hypertension, renal tubular dysfunction, etc., and severe cases can lead to renal failure. Epidemiological data show that the incidence of UAN is increasing year by year worldwide. The increase of uric acid level can lead to the continuous deterioration of kidney function, causing changes in body mass index, blood pressure and proteinuria-creatinine ratio. More and more studies have shown that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61K31/122A61P19/06A61P13/12A61P13/04
CPCA61K31/122A61K31/58A61K2300/00
Inventor 陈君周美樊沁陆萌
Owner CHINA PHARM UNIV
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