Synthesis method of stable isotope 18O labeled nipagin ester

Abstract

The invention relates to a synthesis method of stable isotope 18O labeled nipagin ester. The synthesis method comprises the following steps: taking stable isotope 18O-water as a raw material to reactwith benzoic acid-4-diazosulfate by utilizing different feeding amounts of the 18O-water; carrying out column chromatography silica gel separation to obtain an intermediate product p-hydroxy benzoic acid-18O or p-hydroxy benzoic acid-18O2 respectively; then enabling the intermediate product to be subjected to condensation reaction with a hydroxyl compound under the condition of a catalyst and an organic solvent; finally, separating and purifying to obtain a target product nipagin ester-18O or nipagin ester-18O2. According to the synthesis method provided by the invention, the prepared 18O labeled nipagin ester series product has the advantages that the chemical purity is higher than 98 percent and the abundance of stable isotopes is higher than 95 percent atom 18O; the product can sufficiently meet a mass spectrum detection internal standard substance for detecting content of nipagin ester type preservatives in foods and cosmetics.

Description

technical field [0001] The invention belongs to the technical field of stable isotope-labeled compound synthesis, in particular to a stable isotope 18 Synthetic method of O-labeled parabens. Background technique [0002] In 1932, paraben ester was officially approved as an antifungal agent and fungicide for use in food, and later it was used in cosmetics, medicine and other fields. Among them, in the food industry, paraben esters, as internationally recognized broad-spectrum and high-efficiency food preservatives, have been approved for use in food by the United States, Europe, Japan, Canada, China, Russia and other countries. Currently, they are mainly used in Condiments such as soy sauce and vinegar, pickled products, baked food, sauce products, beverages, rice wine, and fruit and vegetable preservation. China Food Additives Use Hygienic Standard (GB2760-2007) also stipulates that ethylparaben, propylparaben and sodium methylparaben, sodium ethylparaben and sodium propyl...

AI Technical Summary

Problems solved by technology

There are also research reports that paraben will accumulate in the human body, increasing the risk of breast cancer and uterine cancer in women, and a large number of paraben residues are found in the pathological sections of a large number of breast cancer patients

What's more serious is that pregnant women who use excessive paraben ester preservatives can cause genital deformities in newborns

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