Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tribonema and culture and application thereof

A technology of yellow silk algae and algae strains, applied in the fields of application, single-cell algae, animal feed, etc., can solve the problems of slow oil accumulation, unsuitable for production, and few research records, etc., and achieve the effect of fast oil accumulation

Active Publication Date: 2018-04-20
ENN SCI & TECH DEV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing reports, there are 28 species in this genus, and there are only 17 species and 2 varieties in my country, and there are few related research records, and it is generally believed that the growth rate of Xanthophyllum is slow, it cannot continue to be cultured for a long period, and the oil accumulation rate is also slow. It is not suitable for the production of large-scale algae products, so there have been no reports on the large-scale cultivation and industrial application of Xanthophyllum

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tribonema and culture and application thereof
  • Tribonema and culture and application thereof
  • Tribonema and culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Screening and molecular identification of Xanthophyllum ENN172

[0035] 1. Screening of Xanthophylla ENN172 (Tribonema sp.)

[0036] In autumn, water samples were taken from lakes in northeastern Inner Mongolia. In the laboratory, BG11 medium diluted 1 / 10 was added to the water samples, enriched and cultured for 2-3 days under natural light and room temperature, and treated with sterile deionized water. The samples were diluted, and single algae filaments were taken for purification and culture in a 24-well plate. The medium was BG11 medium, the culture temperature was 25°C, and the light was natural light. The purified algae filaments in the orifice plate were transferred to the Erlenmeyer flask for expansion culture, and finally the pure algae strain of ENN172 was obtained.

[0037] Table 1BG11 medium composition

[0038] substance name

Amount per liter of deionized water

NaNO 3 (sodium nitrate)

1.5g

K 2 HPO 4 ·3H 2 O...

Embodiment 2

[0062] The indoor cultivation of embodiment 2ENN172 algal strain

[0063] In a column reactor with an inner diameter of 5cm and a height of 60cm, cultured with sterile BG11 medium, the culture temperature was 25°C, the initial inoculation concentration was about 1g / L, and a mixture of filtered air and carbon dioxide was introduced. gas, wherein the content of carbon dioxide is 1-2% of the total mixed gas. The initial light intensity is 120umol / m 2 / s, in the later stage, according to the growth of biomass, the light intensity is increased to 200 umol / m 2 / s. Samples were taken every day for determination of biomass and fatty acid content in cells for a total of 4 days of culture.

[0064] Biomass determination method: Accurately measure 50 mL of algal liquid, filter it with a glass fiber membrane with a known initial weight after drying (pore size is 0.45um), rinse with 150 mL of deionized water for three times to remove ash, and then remove the attached algal cells. The g...

Embodiment 3

[0066] The oil production of embodiment 3ENN172 algal strain

[0067] The ENN172 algal species at the end of exponential growth was inserted into a column reactor with an inner diameter of 5 cm and a height of 60 cm. The initial inoculation amount of algal cells was about 1.5 g / L, and the medium used was BG11 medium deficient in nitrogen and phosphorus. The temperature is 25℃, and the initial light intensity is 150umol / m 2 / s, the subsequent light intensity is gradually increased to 200umol / m 2 / s, other parameters are the same as in Example 2. Samples were taken every day to measure the fatty acid content in the cells, and the biomass was measured every two days for a total of 4 days of culture. The measurement results are shown in 4.

[0068] Composition and content determination method of fatty acid: Weigh 25 mg of freeze-dried sample, add 2 mL of 2% H 2 SO 4 Anhydrous methanol-toluene solution. After filling with nitrogen, it was heated at 80°C for 2 hours. Then add ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Cell concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a tribonema and culture and application thereof. The tribonema is named as ENN172 (Tribonema sp.), and is already preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 27, 2017, and the preservation number is CGMCC No.14712. The tribonema can be applied in the fields of carbon dioxide emission reduction, wastewater treatment, biofuel production, food, nutrient health-care products, pharmaceutical products, cosmetics, chemical products, feed and bait.

Description

technical field [0001] The present invention relates to a strain of Xanthophyllum sp. and its cultivation and application. Specifically, the present invention relates to a strain of Xanthophyllum sp. , nutrition and health products, pharmaceutical products, cosmetics, chemicals, feed, and bait applications. Background technique [0002] While the social economy is developing rapidly, the greenhouse effect and energy crisis are also coming one after another, which are threatening global security. The capture and utilization of carbon dioxide has risen from an environmental issue to an international political issue, and has become the focus of many governments. Various countries have formulated ambitious emission reduction targets and promulgated support policies for new energy sources, especially biofuels. Biofuels have gone through three stages of development: the first generation of biofuels is derived from corn, soybeans, sugarcane, sugar beets, vegetable oils, and anima...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/12C12P7/64C12P19/04C02F3/32A23K10/16C12R1/89
CPCA23K10/16C02F3/322C12P7/6427C12P7/6463C12P19/04C12N1/125C12R2001/89
Inventor 白雪梅吴洪王慧岭尹顺吉王琳杨巧利李青刘芳伟陈昱朱振旗
Owner ENN SCI & TECH DEV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com