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Xanthone Schiff base modified graphene quantum dots CPCG as well as preparation method thereof and application in preparing sodium glutamate detection reagent

A technology of xanthone Schiff base and graphene quantum dots, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of slow development, complex synthesis methods, and low solubility of compounds , to achieve the effect of convenient operation, simple synthesis method and high selectivity

Inactive Publication Date: 2018-04-24
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the development of these methods is relatively slow due to the low solubility of the compounds in water or the complicated synthesis methods.

Method used

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  • Xanthone Schiff base modified graphene quantum dots CPCG as well as preparation method thereof and application in preparing sodium glutamate detection reagent
  • Xanthone Schiff base modified graphene quantum dots CPCG as well as preparation method thereof and application in preparing sodium glutamate detection reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Synthesis of Xanthone Schiff Base (abbreviation: CP)

[0021] (1) Place 1 g of chitosan (viscosity 100-200 mPa.s) in 100 mL aqueous solution containing 1% acetic acid, stir at a constant speed for 24 hours, then transfer the solution to a three-necked bottle, drop by drop Add 100 mL of ethanol solution containing 0.3 g of 1-chloro-4-propoxythioxanth-9-one, and then add 3 mL of glacial acetic acid dropwise, and heat and stir the mixture at 70 ºC for 20 h, stop stirring, and cool After reaching room temperature, neutralize with NaOH solution until the solution becomes neutral, filter, wash, and dry to obtain compound CP.

[0022] (2) Place 1.5 g of chitosan (viscosity 100-200 mPa.s) in 100 mL aqueous solution containing 5% acetic acid, stir at a constant speed for 12 hours, transfer the solution to a three-necked bottle, drop by drop Add 150 mL of ethanol solution containing 0.1 g of 1-chloro-4-propoxythioxanth-9-one, and then add 2 mL of glacial acetic acid dropwise, an...

Embodiment 2

[0024] Synthesis of compound CPCG

[0025] (1) Put 0.1 g of compound CP in 100 mL of acetic acid aqueous solution with a pH of 6, and stir until it is uniformly dispersed. Take 30 mL of 2.0 mg / mL graphene quantum dot aqueous solution in a 100 mL beaker, add 0.1 mL of N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethyl The mixed solvent of carbodiimide hydrochloride was used as a catalyst. After standing still for 10 minutes for activation, the above-mentioned uniformly dispersed CP solution was added dropwise, heated in a water bath at 40°C for 10 minutes with ultrasonic waves, heated in a water bath at 55°C for 5 hours, and then kept away from room temperature. After light stirring for 24 hours, the product was placed in a dialysis bag with a molecular weight of 1000 and dialyzed in 1000 mL deionized water for three days, and the water was changed every 3 hours to obtain the xanthone Schiff used for the detection of sodium glutamate Alkali-modified graphene quantum do...

Embodiment 3

[0027] Embodiment 3 (selective experiment)

[0028] In the fluorescence experiment, the compound CPCG was made into a 0.03 mg / mL aqueous stock solution. Glycine, glucose, glucosamine, sodium glutamate, maltose, lactose, sucrose, fructose and other substances were used as biomolecules. All experimental solutions were newly configured. And experiment now. Emitting at 426 nm, the biomolecules were tested separately. In the experiment, 2.5 mL of stock solution was taken, and 1M biomolecules solution was added respectively. Test its fluorescence spectrum.

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Abstract

The invention discloses xanthone Schiff base modified graphene quantum dots CPCG as well as a preparation method thereof and application in preparing a sodium glutamate detection reagent. The preparation method is characterized by introducing the xanthone Schiff base into nano quantum dots which are strong in water solubility, specifically for example, introducing Schiff base which is synthesizedby enabling 1-chloro-4-propoxy thioxanthene-9-ketone to react with chitosan to the graphene quantum dots, thus obtaining the xanthone Schiff base modified graphene quantum dots which are strong in water solubility and high in sodium glutamate selectivity. A synthesis method of the xanthone Schiff base modified graphene quantum dots is simple, the conditions are mild, a product can be easily obtained, a good effect can be obtained by applying the compound to sodium glutamate detection, and the compound cannot be affected by other conventional symbiotic biomolecules such as matters of glycine, arginine, methionine, glucose, glucosamine and the like, and high selectivity is obtained; a fluorescence spectrophotometer is convenient to operate, and a fluorescent signal of a sample is obvious.

Description

technical field [0001] The invention relates to the technical field of molecular detection of the amino acid histidine, which is necessary for the optical detection of human body and mammals, in particular to a graphene quantum dot CPCG modified by xanthone Schiff base and its preparation method And the application on the preparation of sodium glutamate fluorescence detection reagent. Background technique [0002] L-sodium glutamate is an excitatory neurotransmitter in the central nervous system of vertebrates. It is a potent neuroexcitatory amino acid and is associated with certain behaviors, such as aggressive behavior, visual task learning, muscle stiffness and retrograde behavior. Forgetting ([Compagnone, D., Federici, G., Massoud, R., et al. Clin. Chem. 1992, 38, 2306–2310. J Gordon,.A. Nat. Neurosci. 2010, 13, 2–4 . Jamal, M., Hasan, Mathewson, M., A., et al. Biosens. Bioelectron. 2013, 40, 213–218.). L-sodium glutamate also has important uses in food processing and c...

Claims

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Application Information

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IPC IPC(8): C07H17/00C07H1/00C09K11/06B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C07H1/00C07H17/00C09K11/06C09K2211/1088C09K2211/1092G01N21/64
Inventor 程如梅区升举程秀梅姚清清
Owner WENZHOU MEDICAL UNIV
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