Preparation method of micro-encapsulation nanometer hybridized microspheres

A microsphere and microfluidic chip technology, which is applied in the field of preparation of micro-encapsulated nano-hybrid microspheres, can solve the problems of cumbersome preparation process, excessively large particle size of microspheres, inconvenient for controllable embolism, etc., and achieves a simple preparation process. , Process controllable effect

Inactive Publication Date: 2018-05-08
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

In addition, this method of preparing autographic microencapsulated nanospheres obviously has the following disadvantages: (1) the preparation process is cumbersome and needs to be completed in multiple steps; (2) the particle size of the microspheres prepared by the drop method is too large (about 2mm) , which is not conducive to its propulsion in the catheter, and the embolization of smaller blood vessels cannot be achieved; the microspheres obtained by the emulsification method all have the defect of non-uniform particle size, which is not convenient for controllable embolization

Method used

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  • Preparation method of micro-encapsulation nanometer hybridized microspheres

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preparation example Construction

[0033] A method for preparing microencapsulated nanohybrid microspheres provided in the embodiment of the present invention adopts droplet microfluidic technology, and specifically includes the following steps:

[0034] (1) Prepare the oil phase: mix the surfactant and the continuous phase matrix uniformly to get final product. The above-mentioned surfactant is a non-ionic surfactant, preferably Span 80, and its content is between 1.5-5wt / V%. The continuous phase matrix is ​​preferably non-polar high-boiling organic solvents such as liquid paraffin, salad oil, and silicone oil;

[0035] (2) Prepare the water phase: dissolve the trivalent gadolinium salt and / or iron salt in the PVA aqueous solution, mix evenly, it is the water phase, the above-mentioned polyvinyl alcohol concentration is 4-10.0wt%, and the above-mentioned trivalent gadolinium salt is water-soluble One of the water-soluble trivalent gadolinium salts such as nitrate, chloride and sulfate, Gd 3+ The concentration...

Embodiment 1

[0043] A method for preparing microencapsulated nanohybrid microspheres, prepared by using a microfluidic chip, comprising the following steps:

[0044] (1) Preparation of oil phase: add 0.75 g of Span 80 to 50 mL of liquid paraffin, and mix well to obtain a liquid paraffin oil phase containing 1.5 wt / V% Span 80.

[0045] (2) Prepare the water phase: first prepare a 4.0wt% PVA aqueous solution, and add a certain amount of GdCl 3 , so that the concentration is 100mmol / L, which is the water phase.

[0046] (3) Prepare alkaline receiving solution: prepare 0.5 mol / L NaOH ethanol solution.

[0047] (4) Preparation of micro-encapsulated PVA microspheres by microfluidic method: the water phase prepared in step (2) and the oil phase prepared in step (1) were respectively loaded into a 2mL syringe (Bidey Medical Instrument Co., Ltd.) and a 10mL syringe ( In Shanghai Jinta Medical Equipment Co., Ltd.), under the push injection of the micro-injection pump, the flow rate of the oil phas...

experiment example 2

[0053] A method for preparing microencapsulated nanohybrid microspheres, prepared by using a microfluidic chip, comprising the following steps:

[0054] (1) Prepare the oil phase: prepare the salad oil oil phase containing 2.5wt / V% Span 80.

[0055] (2) Preparation of water phase: first prepare 7.5wt% PVA aqueous solution, add a certain amount of FeCl 3 and FeCl 2 , so that the concentration is 50mmol / L, which is the water phase.

[0056] (3) Prepare alkaline receiving solution: prepare 1.0 mol / L NaOH aqueous solution.

[0057] (4) Preparation of micro-encapsulated PVA microspheres by microfluidic method: the water phase prepared in step (2) and the oil phase prepared in step (1) were respectively loaded into a 2mL syringe (Bidey Medical Instrument Co., Ltd.) and a 10mL syringe ( Shanghai Jinta Medical Equipment Co., Ltd.), under the push injection of the micro-injection pump, the flow rate of the oil phase is set to 8000 μL / h, and the flow rate of the water phase is 4000 μ...

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Abstract

The invention discloses a preparation method of micro-encapsulation nanometer hybridized microspheres. The preparation method comprises the following steps: propelling an oil phase and an aqueous phase containing polyvinyl alcohol (PVA) into a micro-channel of a micro-fluidic chip separately by a micro-injection pump by a liquid drop type micro-fluidic technology, collecting the formed liquid drops by an alkaline solution and placing until complete reaction, wherein the oil phase comprises a surfactant and a continuous phase matrix; the aqueous phase also comprises trivalent gadolinium salt and / or iron salt; and the iron salt is a mixture of ferric salt and ferrous salt. According to the method, the PVA aqueous phase containing the trivalent gadolinium salt and / or the iron salt is under the action of interfacial tension and oil phase shear force to obtain uniform liquid drops, the liquid drops and the oil phase enter an alkaline receiving liquid, and the PVA microspheres for encapsulating in-situ formed Gd2O3 and / or Fe3O4 nanoparticles; furthermore, the process is simple and controllable, the obtained microspheres are reasonable and uniform in particle size, important theoretical and practical value is achieved and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to a preparation method of an autographic material for interventional embolism therapy, in particular to a preparation method of a microencapsulated nanohybrid microsphere. Background technique [0002] Transcatheter Arterial Embolization (TAE) is to inject the embolic material into the blood vessel of the target site through a microcatheter to cut off the blood supply of the target site under the monitoring of an X-ray machine or a magnetic resonance imaging machine, so as to achieve the purpose of treatment. Small, simple, safe, effective, less complications, etc., are widely used in the treatment of tumors and arteriovenous malformations and other diseases. [0003] Polyvinyl alcohol (PVA) microspheres are clinically commonly used permanent embolic materials (Osuga K, et al. Inter. J. Clin. Oncol. 2012, 17, 306-315; Semenzim VL, J. Appl. 121,1417-1423), has the advantage...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/06A61L24/00A61L24/02A61K49/08A61K49/12A61K49/18A61K41/00
CPCA61L24/06A61K41/0052A61K49/08A61K49/126A61K49/1821A61L24/001A61L24/02A61L2300/418C08L29/04
Inventor 王芹肖艾杨亚江杨祥良朱艳红
Owner HUAZHONG UNIV OF SCI & TECH
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