Suspension culture method of albizia cells
A technology of suspension culture and cell suspension, applied in the directions of plant cells, tissue culture, biochemical equipment and methods, etc., can solve the problems of unprotected genetic resources of excellent traits, long cycle of sowing and breeding, and unable to meet the market demand of Albizia Julibrissin , to increase the fresh weight of cells, improve the growth rate of cells, and solve the shortage of medicinal materials.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] (1) Acquisition of aseptic explants: choose the axillary buds of Albizia julienne that grow healthy and have no damage by diseases and insect pests as explants, rinse with tap water for 30 minutes, then shake for 2 minutes in an aqueous solution with a volume concentration of 1% detergent, and use sterile Rinse with water; soak for 30s with a volume concentration of 70-75% ethanol solution on the ultra-clean workbench, and then use a mass concentration of 0.1% HgCl 2 Soak in disinfectant for 8 minutes, then rinse with sterile water for 3 times, and blot dry on sterile filter paper;
[0029] (2) Induction culture of Albizia juliensis callus: insert the sterilized axillary buds into the callus induction medium, first culture in dark at 23°C for 10 days, then light for 8 hours every day, and light intensity under the condition of 1000lx For culturing, the callus induction medium is to add 6-BA (ie 6-benzylaminoadenine) 0.1mg / L, 2,4-D (ie 2,4-dichlorophenoxyacetic acid) 0.5...
Embodiment 2
[0035] The influence of embodiment 2PVP on Albizia juliensis cell suspension culture
[0036] After pre-cultivation, single cells or loose cell aggregates of Albizia juliensis in the middle and upper part of the suspension culture medium were selected and transferred to the cell suspension medium added with different hormones. Other operations were the same as in Example 1. The results of the suspension culture of Albizia juliensis cells are shown in Table 1. Show. The results show that the culture medium of different hormone combinations can induce Albizia julienne cell proliferation to varying degrees, but after adding PVP (i.e. polyvinylpyrrolidone) in the culture medium, the browning rate of the cells decreases, the cell growth multiple increases, and the cell growth rate also increases. Among them, PVP added with 50mg / L had the fastest cell growth rate, the cell proliferation multiple reached 2.97 times, and the cell growth rate reached 3.36g / (d·L).
[0037] The effect o...
Embodiment 3
[0039] The influence of the pre-cultivation of embodiment 3 cells on the suspension culture of Albizia juliensis cells
[0040] The non-browning yellow-white callus of Albizia juliensis obtained in step (2) of Example 1 is transferred into the pre-culture liquid medium for pre-cultivation of different days, and other steps are the same as in Example 1. For the results of the suspension culture of Albizia juliensis cells, see Table 2 shows. The results showed that after pre-cultivation for 7 days, the biomass of Albizia juliensis cells subcultured for 3 generations proliferated the most, the cell proliferation multiple was 2.86 times, and the cell growth rate was 3.25g / (d·L).
[0041] Table 2 The effect of pre-cultivation on the suspension culture of Albizia juliensis cells
[0042] Pre-cultivation time (days)
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com