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A compound probiotic agent containing chicken alpha interferon and its preparation method

A technology of compounding probiotics and alpha interferon, applied in the preparation method of peptide, interferon, cytokine/lymphokine/interferon, etc., can solve the problems of single bacterial type, short storage period, poor antiviral effect, etc. To achieve the effect of inhibiting the growth of pathogenic bacteria, improving the growth level and convenient route of administration

Active Publication Date: 2021-06-18
广州市微生物研究所集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the defects and deficiencies of the above-mentioned prior art, provide a compound probiotic agent containing chicken alpha interferon and its preparation method, and solve the problems existing in the existing probiotic agent in the application process of animal husbandry. The bacteria type is single, the anti-virus effect is poor, and the storage period is short, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of chicken interferon alpha powder preparation

[0041] 1. High-density fermentation culture of engineering strains

[0042] (1) Take the recombinant Pichia strain expressing chicken α-interferon (that is, the Pichia engineered strain containing chicken α-interferon mature peptide gene sequence), in 100mL YPD medium, at 27-29°C, 250rpm / Min shaking culture for 17-19 hours; then transfer to 1000mL YPD medium, at 27-29°C, 250rpm / min shaking culture for 17-19 hours, to obtain seed liquid;

[0043] Wherein, the Pichia engineered strain containing chicken α-interferon mature peptide gene sequence is based on the Pichia wild-type strain X-33 as the host cell, and the Pichia constitutive expression plasmid pGAPzα-A as the vector (two Both are commercial strains and plasmids) and were constructed.

[0044] Specifically, the construction method of the Pichia pastoris engineering strain containing the chicken interferon alpha mature peptide gene sequence i...

Embodiment 2

[0055] Example 2 Preparation of Compound Probiotics Containing Chicken Interferon Alpha

[0056] 1. Weigh the following components by mass percentage: 20% chicken alpha interferon powder preparation, 5% Bacillus subtilis powder, 2% Saccharomyces cerevisiae powder, 15% Lactobacillus reuteri powder, Lactobacillus plantarum Powder 20%, Fumei Powder 38%.

[0057] Among them, the number of live bacteria in Bacillus subtilis powder is 100 billion cfu / g, the number of live bacteria in Saccharomyces cerevisiae powder is 5 billion cfu / g, and the number of live bacteria in Lactobacillus reuteri powder is 10 billion cfu / g. The number of viable bacteria in Lactobacillus powder is 20 billion cfu / g.

[0058] 2. The above components are stirred and mixed with a mixer, crushed and passed through a 100-mesh sieve to obtain a compound probiotic agent containing chicken alpha interferon.

Embodiment 3

[0059] Example 3 Preparation of Compound Probiotics Containing Chicken Interferon Alpha

[0060] With reference to Example 2, the distribution ratio of each component of the compound probiotic agent is: chicken alpha interferon powder preparation 15%, Bacillus subtilis bacterial powder 3%, Saccharomyces cerevisiae bacterial powder 3%, Lactobacillus reuteri bacterial powder 5%, Lactobacillus plantarum powder 25%, Fumei powder 49%.

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PUM

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Abstract

The invention discloses a compound probiotic agent containing chicken alpha interferon and a preparation method thereof. Firstly, a method for preparing chicken interferon alpha is provided, which is to add the fermentation liquid of recombinant bacteria expressing chicken interferon alpha to boiled benzoic acid, filter or centrifuge with a ceramic membrane, and then add a certain amount of After the maltodextrin is dissolved, it is spray-dried to obtain the chicken alpha interferon powder preparation. It is further mixed with Bacillus subtilis powder, Saccharomyces cerevisiae powder, Lactobacillus reuteri powder, Lactobacillus plantarum powder and Fumei powder to form a compound probiotic agent containing chicken alpha interferon. The compound probiotic agent can be used as a feed additive for poultry and livestock or directly mixed with water orally. It can regulate the microecological balance of the gastrointestinal tract of animals, inhibit the growth of pathogenic bacteria, significantly improve the growth level of laying chicks and the utilization rate of feed, and play a role Antiviral and immunomodulatory effects can reduce or replace the use of antibiotics, reduce the occurrence of drug-resistant bacteria and solve the problem of drug residues.

Description

technical field [0001] The invention belongs to the technical field of probiotics. More specifically, it relates to a compound probiotic agent containing chicken interferon alpha and a preparation method thereof. Background technique [0002] China is the largest poultry-raising country, but the epidemic of the virus has been perplexing the poultry industry in China, causing huge economic losses to the poultry industry. In recent years, chicken Marek's disease, Newcastle disease, and infectious FA Sacral disease, infectious bronchitis, infectious laryngotracheitis, encephalomyelitis, influenza, duck hepatitis and other highly infectious viral diseases have become local epidemic trends, bringing economic losses of billions of yuan to the poultry industry every year. How to effectively treat poultry tumors and viral infectious diseases has always been one of the major problems in the prevention and control of poultry diseases. [0003] At present, some poultry viral diseases...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/56C07K1/14A23K50/75
CPCA23K10/12A23K10/18A23K40/10A23K50/75
Inventor 曹丁黄魁英李学优甘祥武陈琼黄乐天张宜靖夏枫耿
Owner 广州市微生物研究所集团股份有限公司
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