A photoelectrochemical nucleic acid analysis method for defect-regulated semiconductors
A photoelectrochemical and analytical method technology, which is applied in the field of analytical chemistry to achieve wide application prospects, improved recognition ability and sensitivity, and low background signals.
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Embodiment 1
[0032] (1) Preparation of defect-regulated titania nanoblocks
[0033] Dissolve 12 mg of ferric chloride hexahydrate solid in oleic acid (8 mL), oleylamine (7 mL) and ethanol (5 mL) mixed solvent, then add 5 mmol butyl phthalate and stir for ten minutes, transfer to 35 mL open glass bottle, and finally put the glass bottle into a 100 mL polytetrafluoroethylene reactor with 20 mL ethanol solution, and react at 160°C for 20 hours; after cooling, wash twice with ethanol centrifugation, collect solid ingredients.
[0034] (2) Defect-regulated surface modification of titania nanoblocks
[0035] The hydrophobic titanium dioxide prepared in step (1) was dispersed into a mixed solution of toluene (3 mL), diethylene glycol (20 mL) and polyacrylic acid (2 g), heated slowly to 100 °C, and then heated to 180 °C under reflux for 10 hours, and then alternately centrifuged with ethanol and water for 4 washes to collect the solid components.
[0036] (3) Exonuclease III assists signal ampl...
Embodiment 2
[0043] λ exonuclease-assisted signal amplification for detection of chronic myelogenous leukemia genes
[0044] Disperse the solid modified in step (2) in Example 1 into water to obtain a 4 mg / mL solution. Take 5 μL of the resulting solution and add it dropwise to the surface of the PDDA-modified electrode. NHS aqueous solution (1.5mL), after 40 minutes, washed with 10 mM Tris-HCl buffer (pH 7.4), 2 μM capture probe with amino group (cDNA 2 , 10 μL) onto the surface of the electrode and left overnight at 4°C; blocked with 1 mM ethanolamine for 1 hour and washed with Tris-HCl buffer.
[0045] Mix and shake the hairpin nucleic acid with nano silver (AgNPs) sol overnight to form DNA-AgNPs nucleic acid probe (pDNA 2 );
[0046] Dissolve 2 μL of pDNA in a centrifuge tube 2 (0.5 μM), 5 μL of λ exonuclease (0.5 U), 4 μL of different concentrations of tDNA 2 Mix, shake and react at 37°C for 60 minutes; then heat the centrifuge tube in a water bath to 80°C and cool it to room temp...
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