Lucid ganoderma skin care composition with anti-aging and brightening effects and application thereof
A skin care composition and composition technology, applied in the field of Ganoderma lucidum skin care composition, can solve problems such as unsatisfactory effects
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Embodiment 1
[0078] Example 1. Preparation of Ganoderma lucidum, Astragalus, Mushroom, Polygonum cuspidatum extract and preparation of the composition
[0079] Take the four traditional Chinese medicines of Ganoderma lucidum, Astragalus, Junchen and Polygonum cuspidatum, and prepare the mixed extract of Ganoderma lucidum, Astragalus, Junchen and Polygonum cuspidatum according to the following method:
[0080] (1) The grinder grinds each Chinese medicine into a finely crushed powder and passes it through a 60-mesh sieve, screens the finely divided part of the Chinese medicine through the mesh for use, and mixes the Chinese medicine powder separately through the sieve;
[0081] (2) According to the ratio of 1:30 for the ethanol extraction determined by the orthogonal experiment, weigh an appropriate amount of 95% ethanol reagent to soak the Chinese medicine for 1 hour, place an electric heating mantle for heating, and heat the temperature to 78°C. Turn on the cold circulating water for heating Refl...
Embodiment 2
[0086] Example 2: DPPH free radical scavenging experiment
[0087] Experimental principle:
[0088] The DPPH radical has a single electron, has a strong absorption at 517nm, and its alcohol solution is purple. When there is a free radical scavenger, its absorption gradually disappears due to its one-electron pairing, and its fading degree is quantitatively related to the number of electrons it accepts, so a spectrophotometer can be used for rapid quantitative analysis.
[0089] Main reagents:
[0090] DPPH (Shanghai Yuanye Biotechnology Co., Ltd., ≥97%); Ascorbic acid (Vc) and methanol are all domestically produced analytically pure.
[0091] Sample Preparation:
[0092] The sample was diluted 20 times with purified water to obtain a clear liquid for testing.
[0093] Experimental results:
[0094] According to the method described in Example 1, the ethanol extract extracts of the four Chinese herbal medicines of Ganoderma lucidum, Astragalus, Mushroom and Polygonum cuspidatum were prepar...
Embodiment 3
[0101] Example 3: Tyrosinase activity inhibition experiment
[0102] 1 part of Ganoderma lucidum, 2 parts of Astragalus, 2 parts of Polygonum cuspidatum, and 2 parts of Bacteria cuspidatum in a suitable ratio of four Chinese medicines (sample No. 8) (the following two Chinese medicines and three Chinese medicines for samples No. 1-7 below have a formula of 1:2 and 1:2:2), the grinder grinds into finely divided powder, and extracts with pure ethanol reagent under hot reflux, recovers the ethanol, and obtains the extract dissolved in butanediol. See Example 1 for the specific extraction method.
[0103] Experimental principle:
[0104] Tyrosinase can catalyze the substrate tyrosine to generate dopaquinone, and then through a series of reactions finally generate melanin. The tyrosinase catalyzed reaction is considered to be the rate-limiting step of melanin production, so it can inhibit tyrosinase activity The substance is considered to have potential whitening activity. Dopaquinone ...
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