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Improvement method for antenatal gene chip detection

A gene chip and gene technology, applied in the field of detection of fetal chromosome copy number abnormalities or single nucleotide polymorphism abnormalities, can solve problems such as lack of known content

Pending Publication Date: 2018-05-25
翁炳焕
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, in the face of prenatal gene chip detection of rare genetic diseases, which are precious and rare in case samples and difficult to be amplified industrially, there is a lack of standards with known content that can be used as standards and quality controls

Method used

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  • Improvement method for antenatal gene chip detection
  • Improvement method for antenatal gene chip detection
  • Improvement method for antenatal gene chip detection

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Experimental program
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Embodiment Construction

[0014] figure 1 It is a schematic diagram of CD138 monoclonal antibody fusion of the present invention.

[0015] figure 2 It is a schematic diagram of SPA synergistic double antibody fusion of the present invention.

[0016] image 3 It is the real picture of cell fusion of the present invention

[0017] Such as figure 1 , 1 represents myeloma cells with CD138 monoclonal antibody Fab receptor, 2 represents CD138 monoclonal antibody (IgG), 3 represents gene mutation cells with CD138 monoclonal antibody Fc receptor, and the other Fab end of CD138 monoclonal antibody (IgG) is also It can be combined with a myeloma cell, and the cells to be fused are connected together by CD138 monoclonal antibody, which is beneficial to fusion under the action of PEG.

[0018] Such as figure 2 , 1 represents the Staphylococcus aureus protein A (SPA) with IgGFc receptor, 2 represents the monoclonal antibody of the cells to be fused with numbers 6 and 7, 3 represents the monoclonal antibodi...

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Abstract

The invention discloses an improvement method for antenatal gene chip detection and relates to the field of medicine. The method is characterized in that according to surface markers of genome abnormal cells and myeloma cells, the effects of a specific antibody and an antibody bridge connection thereof are involved, cells are easily in contact with cell membranes and is specifically fused with thecell membranes under the effect of the antibody, the valid fusion rate is increased, and invalid fusion is reduced; a cell fusion method is designed, full-abnormal genomes are implanted into tumor cells and amplified along with amplification of the tumor cells, and then monoclonal hybridoma cell strains with abnormal genomes are screened out and prepared, subjected to abnormal gene detection, prepared into standard reference cells, and used for replacing an existing gene detection comparison database, and accordingly the improvement method for detection comparison, in-chamber quality controland among-chamber quality control of detected mutant genes and the antenatal gene chip detection is established.

Description

technical field [0001] The invention relates to an improved prenatal gene chip detection method in the medical field, which is mainly applied to the detection of fetal chromosome copy number abnormality or single nucleotide polymorphism abnormality. Background technique [0002] Gene chip is a recently developed high-throughput gene expression analysis technology, which is to print dot matrix on the surface of nylon membrane or synthesize thousands of oligonucleotides representing different genes as probes on the surface of silicon slide. Complementary combination with radioactive isotope or fluorescein-labeled DNA or cDNA in the material to be tested, using autoradiography or laser confocal microscope to scan hybridization signals, and finally centralized analysis and processing by computer. The obtained hybridization signal intensity and distribution pattern can reflect the expression of related genes in the tested sample. [0003] The application of gene chips depends on...

Claims

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Application Information

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IPC IPC(8): C12N5/16C12Q1/6883
CPCC12N5/16C12Q1/6883C12Q2600/158
Inventor 翁炳焕李兰娟
Owner 翁炳焕
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