Liver stem cell injection and preparation method thereof

A technology for liver stem cells and injection, which is applied in the field of biomedicine, can solve the problems of allergic reactions, poor cell preservation ability, and inability to guarantee cell activity for a long time, and achieves the effect of low cost and better treatment effect.

Inactive Publication Date: 2018-06-01
重庆斯德姆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, unlike ordinary biological products and medicines, the active substance of stem cell injection is cells, and the activity of cells will decrease rapidly in an in vitro environment. The traditional method uses sodium chloride injection containing human serum albumin to preserve liver stem cells. Within 8-24 hours, the cell viability will drop significantly, and the cell viability cannot be guaranteed for a long time. For example, the liver stem cell preservation solution disclosed in the Chinese patent CN102948413B is mainly composed of human serum albumin, sodium chloride, sodium gluconate, sodium acetate, chloride Potassium, magnesium chloride and heparin calcium, the hepatic stem cells are resuspended in the preservation solution to form an injection, and the cell preservation ability is poor
A large number of studies have found that using a preservation solution containing fetal bovine serum or cell culture medium can solve the problem of decreased cell activity, but when cells contact fetal bovine serum, they will endocytose fetal bovine serum, which will cause changes in the expression characteristics of certain proteins in cells. It may cause allergic reactions after reinfusion into the human body, and the serum contains a large number of unknown components, so it cannot be used clinically

Method used

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  • Liver stem cell injection and preparation method thereof
  • Liver stem cell injection and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0020] A kind of hepatic stem cell injection for treating diabetes, each ml injection mixture contains 1×107 hepatic stem cells and 1×103 umbilical cord blood stem cells; the injection mixture consists of the following mass percentage components: 2.1% benzyl alcohol, 3.3 % asparagine, 3.4% glutamine, 4% isoleucine, 4.4% proline, 0.9% hyaluronic acid, 3.7% transferrin, 4% Polygonatum polysaccharide, 0.4% taurine and 0.7% progesterone; the balance is normal saline.

Embodiment 2

[0022] A hepatic stem cell injection for treating diabetes. Each ml of the injection mixture contains 3×105 hepatic stem cells and 1×104 umbilical cord blood stem cells; the injection mixture consists of the following components by mass percentage: 1.3% benzyl alcohol, % asparagine, 4.6% glutamine, 4.1% isoleucine, 3.4% proline, 0.7% hyaluronic acid, 4.5% transferrin, 4.1% Polygonatum polysaccharide, 0.5% taurine and 0.8% progesterone; the balance is normal saline.

[0023] Preparation:

[0024] 1) Culture of liver stem cells;

[0025] 2) Cultivation of umbilical cord blood stem cells;

[0026] 3) dissolving benzyl alcohol, asparagine, glutamine, isoleucine, proline, hyaluronic acid, transferrin, polygonatum polysaccharide, taurine and progesterone in normal saline to prepare a compound solution, Pre-cool at 4°C for later use;

[0027] 4) Resuspend the hepatic stem cells obtained in step 1) and the cord blood stem cells obtained in step 2) in the compound solution obtained...

Embodiment 3

[0029] A hepatic stem cell injection for treating diabetes. Each ml of the injection mixture contains 1×106 liver stem cells and 7×103 umbilical cord blood stem cells; the injection mixture is composed of the following mass percentage components: 1% benzyl alcohol, 3.5 % asparagine, 4.9% glutamine, 4.8% isoleucine, 3.6% proline, 0.8% hyaluronic acid, 3.5% transferrin, 3.9% Polygonatum polysaccharide, 0.4% taurine and 1% progesterone; the balance is normal saline;

[0030] Preparation:

[0031] 1) Culture of liver stem cells;

[0032] 2) Cultivation of umbilical cord blood stem cells;

[0033] Preparation:

[0034] 1) Culture of liver stem cells;

[0035] 2) Cultivation of umbilical cord blood stem cells;

[0036] 3) dissolving benzyl alcohol, asparagine, glutamine, isoleucine, proline, hyaluronic acid, transferrin, polygonatum polysaccharide, taurine and progesterone in normal saline to prepare a compound solution, Pre-cool at 4°C for later use;

[0037] 4) Resuspend th...

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Abstract

The invention relates to a liver stem cell injection and a preparation method. The injection is prepared by composing liver stem cells, umbilical cord blood stem cells, benzyl alcohol, asparagine, glutamine, isoleucine, proline, hyaluronic acid, transferrin, polygonatum polysaccharide, taurine, and progesterone according to a certain weight ratio. In the liver stem cell injection provided by the invention, the liver stem cells and umbilical cord blood stem cells are coordinated mutually to have better therapeutic effects on treating diabetes; the injection formula provided by the invention canmaintain the activity of liver stem cells for a long time, and the injection has no toxic and side components, is safe and effective, and low in cost, and can be directly applied to the clinical injection about diabetes.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a liver stem cell injection and a preparation method thereof. Background technique [0002] In the early 20th century, some scholars speculated through some experimental phenomena that the mature liver has a strong regenerative capacity, because there is a group of "ancestor" cells that can differentiate into liver cells and bile duct cells. At present, people have formed a broad consensus on the existence of liver stem cells. It is a kind of stem cells with self-renewal ability and multi-directional differentiation potential. It can produce stem cells with the same phenotype and genotype as itself through its own symmetric and asymmetric division. , can also produce cells of different developmental stages and different differentiation directions in liver tissue, such as hepatocytes, bile duct epithelial cells and pancreatic epithelial cells. With the in-depth understanding of its cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K47/36A61K35/28A61P3/10
Inventor 赵翔熊华强熊荣骞杨仁淑
Owner 重庆斯德姆生物技术有限公司
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