Unlock instant, AI-driven research and patent intelligence for your innovation.

A high-throughput photoelectrochemical tyrosinase sensor

A tyrosinase, photoelectrochemical technology, applied in the fields of material electrochemical variables, scientific instruments, material analysis by electromagnetic means, etc., can solve the problems of reduced enzyme activity, loss, unfavorable high-throughput detection, etc., to achieve high selectivity The effect of high sex and high sensitivity

Active Publication Date: 2019-09-03
JIANGNAN UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these traditional PEC enzyme sensors usually immobilize natural enzymes on the electrode surface for detection, which has some defects: ① During the process of enzyme immobilization on the electrode surface through electrostatic adsorption, covalent binding, embedding and cross-linking, etc. It may lead to the reduction / loss of enzyme activity; ②After the enzyme is immobilized on the electrode surface as a biomacromolecule, it will generate a large steric hindrance, which will inhibit the signal substance (that is, the reactant / product involved in the enzyme reaction process) to a certain extent. The contact with the photoelectric active material on the electrode surface will reduce the sensitivity of the sensor; ③The immobilization of the enzyme on the electrode surface leads to either a biological reaction or a photoelectrochemical detection during a period of time during photoelectric analysis, which is not conducive to the realization of high-throughput detection
Unfortunately, so far, there is only one report on an enzyme sensor based on a photocathode: the literature uses the reduction of the NiO / CdS photocathode current to detect
However, the sensitivity and selectivity of this quenching biosensor are not particularly satisfactory compared to enhanced biosensors
In addition, how to achieve signal-enhanced, high-throughput detection that does not require enzyme immobilization on the photoelectrode surface remains a great challenge.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A high-throughput photoelectrochemical tyrosinase sensor
  • A high-throughput photoelectrochemical tyrosinase sensor
  • A high-throughput photoelectrochemical tyrosinase sensor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] a. Mix 12 μL of thioglycolic acid and 25 mL of 4.0 mmol / L Pb(CH 3 COO) 2 The solution was mixed and stirred, and the pH of the solution was adjusted to 8 with 1mol / L NaOH solution; then deoxygenated for 30min, and then 2.0mL of NaOH with a concentration of 0.02mol / L was added 2 S aqueous solution, deoxygenation reaction at 25°C for 4 hours to obtain water-soluble PbS quantum dots;

[0023]b. Modify PbS quantum dots and chitosan on the surface of the electrode sequentially: immerse the pretreated ITO glass sheet in a solution of 2% PDDA polymer containing NaCl, wash it after 10 minutes; then immerse it in water-soluble PbS quantum dots Spot the solution, take it out after 30 minutes and wash it; prepare a chitosan solution with a mass concentration of 2wt% with pH=4.0 acetate buffer, and then dilute it to a concentration of 0.5wt% with pH=7.0 acetate buffer; draw 20uL after dilution Add the chitosan solution dropwise on the PbS quantum dot electrode, dry at room temper...

example 2

[0026] a. Mix and stir 9 μL of thioglycolic acid and 25 mL of 4.0 mmol / L solution, adjust the pH of the solution to 8 with 1 mol / L NaOH solution; then deoxygenate for 30 minutes, and then add 2.0 mL of NaOH with a concentration of 0.02 mol / L 2 S aqueous solution, deoxygenation reaction at 0°C for 4 hours to obtain water-soluble PbS quantum dots;

[0027] b. Modify PbS quantum dots and chitosan on the surface of the electrode sequentially: immerse the pretreated ITO glass sheet in a solution of 2% PDDA polymer containing NaCl, wash it after 10 minutes; then immerse it in water-soluble PbS quantum dots Spot the solution, take it out after 30 minutes and wash it; prepare a chitosan solution with a mass concentration of 2wt% with pH=4.0 acetate buffer, and then dilute it to a concentration of 0.5wt% with pH=7.0 acetate buffer; draw 20uL after dilution Add the chitosan solution dropwise on the PbS quantum dot electrode, dry at room temperature, and set aside;

[0028] c. Determina...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Quinone compounds generated by a tyrosinase catalytic oxidation phenolic compound can be covalently bonded to the surface of a PbS quantum dot modified photoelectrode via a quinone-ammonia reaction; chitosan is deposited on the photoelectrode; the quinone compounds serve as electron acceptors and greatly inhibit photoproduction electron-hole recombination of a PbS quantum dot; and therefore, the cathode photocurrent is greatly increased. A photoelectrochemical enzyme sensor specific to activity detection of tyrosinase is constructed by using the principle. The sensor has high selectivity of aphotoelectric cathode and the advantages of no need for enzyme immobilization, high throughput and high sensitivity. A new strategy is provided for development of the photoelectrochemical enzyme sensor of the cathode.

Description

[0001] Technical field: [0002] The invention relates to the field of nanometer analysis and detection, in particular to the application of a photocathode in a high-throughput tyrosinase sensor. [0003] Background technique: [0004] The establishment of simple and sensitive sensors based on enzyme-catalyzed reactions has always been an important research content in the field of analysis and detection. Based on the high efficiency, mildness and specificity of the enzyme reaction, the enzyme sensor is used in the detection of enzyme activity, the detection of the substrate / inhibitor of the enzyme reaction, and most importantly, in many biological affinity reactions (such as DNA hybridization, immune reaction, Nucleic acid aptamer-protein recognition reaction, etc.)-based biological analysis [Créminon C, Taran F. Chem. Commun. 2015, 51:7996-8009.] has a wide range of applications. Therefore, enzyme sensors are of great significance in basic scientific research and clinical dia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327
Inventor 王光丽袁芳王红董玉明
Owner JIANGNAN UNIV