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Selenium-enriched plant nutrient solution containing polyglutamic acid and preparation method thereof

A technology of polyglutamic acid and selenium-enriched plants, which is applied to the preparation of organic fertilizers, biochemical equipment and methods, and methods based on microorganisms. problems, to achieve the effect of reducing the use of chemical fertilizers and pesticides, eliminating energy consumption, and mild preparation conditions

Inactive Publication Date: 2018-06-22
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current nutrient solution sprayed on the leaves has the following problems. One is that the sprayed solution dries rapidly on the leaves, which affects absorption; Improper fertilization preparation can easily cause leaf burns; fourth, the residual effect time of foliar fertilization is short, and it needs to be sprayed many times, which is labor-intensive.
Fifth, the foliar nutrient solution generally only has a nutritional function and cannot prevent pests and diseases
Although the liquid fertilizer of this invention can increase the selenium content in crops, improve quality and yield, it does not have the effect of preventing and controlling diseases

Method used

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  • Selenium-enriched plant nutrient solution containing polyglutamic acid and preparation method thereof
  • Selenium-enriched plant nutrient solution containing polyglutamic acid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of polyglutamic acid fermentation broth: Bacillus subtilis FRD518CGMCC was inoculated in a conical flask equipped with seed medium, the inoculation amount was 1%, and cultured to OD at 38°C and 180r / min shaking table 600 Reached 1.8. The prepared seed solution was inoculated into the fermentation medium with an inoculation amount of 5%, and cultured at 38° C. with aeration and stirring for 42 hours to obtain a fermentation solution.

[0032] The components of each medium are as follows:

[0033] Seed medium: glucose 10g / L, yeast powder 5g / L, ammonium sulfate 5g / L, MgSO 4 0.3g / L, pH 7.2, 100mL medium in a 500mL Erlenmeyer flask, sterilized at 115°C for 20min.

[0034] Fermentation medium: peptone 10g / L, yeast extract 10g / L, ammonium sulfate 10g / L, sodium glutamate 50g / L, citric acid 2g / L, MgSO 4 0.2g / L, K 2 HPO 4 2g / L, glucose 100g / L, pH 7.2, sterilized at 115°C for 20min.

[0035] (2) Preparation of selenium-enriched plant nutrient solution cont...

Embodiment 2

[0038] (1) Preparation of fermentation broth: Bacillus subtilis FRD518CGMCC was inoculated in a Erlenmeyer flask equipped with seed medium, the inoculum amount was 1%, and cultured to OD at 36°C and 220r / min shaking table 600Reach 2.2. The prepared seed solution was inoculated into the fermentation medium with an inoculation amount of 2%, and cultured at 36° C. with aeration and stirring for 48 hours to obtain a fermentation solution.

[0039] The components of each medium are as follows:

[0040] Seed medium: glucose 6g / L, yeast powder 3g / L, ammonium sulfate 10g / L, MgSO 4 1.0g / L, pH 7.5, 100mL medium in a 500mL Erlenmeyer flask, sterilized at 115°C for 20min.

[0041] Fermentation medium: peptone 30g / L, yeast extract 30g / L, ammonium sulfate 20g / L, sodium glutamate 50g / L, citric acid 5g / L, MgSO 4 0.5g / L, K 2 HPO 4 4g / L, glucose 200g / L, pH 7.5, sterilized at 115°C for 20min.

[0042] (2) Preparation of selenium-enriched plant nutrient solution containing polyglutamic ac...

Embodiment 3

[0045] (1) Preparation of fermentation broth: Bacillus subtilis FRD518CGMCC was inoculated in a Erlenmeyer flask equipped with seed medium, the inoculum amount was 1%, and cultured to OD at 35°C and 200r / min shaking table 600 Reached 1.6. The prepared seed solution was inoculated into the fermentation medium with an inoculation amount of 3%, and cultured at 35° C. with aeration and stirring for 54 hours to obtain a fermentation solution.

[0046] The components of each medium are as follows:

[0047] Seed medium: glucose 5g / L, yeast powder 4g / L, ammonium sulfate 2g / L, MgSO 4 0.5g / L, pH 7.3, 100mL culture medium in a 500mL Erlenmeyer flask, sterilized at 115°C for 20min.

[0048] Fermentation medium: peptone 5g / L, yeast extract 25g / L, ammonium sulfate 5g / L, sodium glutamate 70g / L, citric acid 4g / L, MgSO 4 0.1g / L, K 2 HPO 4 1g / L, glucose 180g / L, pH 7.2, sterilized at 115°C for 20min.

[0049] (2) Preparation of selenium-enriched plant nutrient solution containing polyglu...

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Abstract

The invention provides a selenium-enriched plant nutrient solution containing polyglutamic acid and preparation method thereof. The nutrient solution is prepared from acid polyglutamic acid fermentation liquor, potassium nitrate, calcium nitrate, sulfuric acid, ferrous sulfate, manganese sulfate, zinc sulfate, boric acid, phosphate, alum and the like; the acid polyglutamic acid fermentation liquoris prepared by adding the seleninic acid in the polyglutamic acid fermentation liquor until the fermentation liquid pH is 2-4. The polyglutamic acid fermentation liquor is directly used as the raw material, the nutrient substances in the fermentation liquor are reserved, and the resource is sufficiently utilized, wherein the polyglutamic acid is easy to form hydrogel, so that the covered fertilizer and microelements have obvious sustained-releasing effect, the nutrient absorbing capacity of the crops can be enhanced, the fertilizer efficiency is improved, and the fertilizing amount is reduced; the bacillus subtilis has good preventing effect on the plant disease, and the use of the pesticide can be reduced.

Description

technical field [0001] The invention belongs to the field of high molecular polymer preparation, in particular to a selenium-enriched plant nutrient solution containing polyglutamic acid and a preparation method thereof. Background technique [0002] At present, the current situation of fertilization in my country's agriculture is: single fertilization, large amount of fertilizer, low utilization rate, serious loss of soil nutrients, poor yield increase effect, and damage to the ecological environment. Practice has proved that foliar fertilization has the characteristics of rapid fertilizer efficiency, high fertilizer utilization rate, and low dosage. With foliar fertilization, various nutrients can be quickly absorbed by the leaves of the crops, quickly alleviating the fertilizer shortage of the crops, and maximizing the benefits of fertilizers. [0003] However, the current nutrient solution sprayed on the leaves has the following problems. One is that the sprayed solutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05F17/00C12P13/02C12R1/125C05G3/60
CPCC05B7/00C05F17/00C05G3/00C05G3/60C05G5/20C12P13/02C05C5/02C05C5/04C05D1/00C05D5/00C05D9/02C05F9/04Y02W30/40
Inventor 王庆波苏移山李海军张英华谢鲁光袁艳华马双双刘芳李韩军王绪杰
Owner SHANDONG FREDA BIOTECH
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