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A kind of process of extracting l-isoleucine by fermentation

An isoleucine and process technology, which is applied in the field of L-isoleucine extraction technology in the biological fermentation industry, and can solve the problem of inability to meet the requirements of L-isoleucine production technology, inability to separate heteroamino acids, and difficulty in sewage treatment, etc. problems, to achieve the effect of promoting utilization and transportation, reducing production costs, and prolonging the acid production cycle

Active Publication Date: 2021-04-16
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precipitation method has the advantages of simple operation and high purity of the extracted product. The disadvantage is that the precipitant is a kind of benzene, which is carcinogenic and easy to remain in the product. Moreover, the operation process is strong acid extraction, which has potential safety hazards. At the same time, there are difficulties in sewage treatment. The problem
Although the total membrane method has less waste water, it cannot separate heteroamino acids with a molecular weight close to that of L-isoleucine, resulting in a high level of heteroacids in the extracted product. High-purity L-isoleucine can only be prepared by repeated crystallization.
The ion exchange method has the advantages of low cost, convenient operation, good extraction effect, simple equipment, etc., but the organic wastewater generated by this method causes higher post-treatment costs
At present, a single extraction method can no longer meet the requirements of L-isoleucine production technology, and it is necessary to find a more economical and effective method to solve this technical problem

Method used

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  • A kind of process of extracting l-isoleucine by fermentation

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Experimental program
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Effect test

Embodiment 1

[0035] A process for fermenting and extracting L-isoleucine, comprising the steps of:

[0036] 1) Corynebacterium glutamicum ATCC14309 seed solution (1×10 8 CFU / mL) was transferred to the fermentation medium according to the inoculum size of 6% for cultivation, the temperature was 30°C, the ventilation rate was 3L / min, and the cultivation time was 60 hours to obtain the L-isoleucine fermentation liquid; Sodium hydroxide aqueous solution is used to control the pH of the fermentation broth to be 7.0, and the residual sugar is controlled at not less than 1.2% by feeding a glucose solution with a concentration of 100g / L;

[0037] Fermentation medium components are: glucose 10%, corn steep liquor 3%, ammonium sulfate 3%, calcium carbonate 1%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.2%, ferrous sulfate 0.001% , VB1 0.0001%, pH 7.0;

[0038] Use a ceramic membrane to filter the L-isoleucine fermentation liquid to separate the fi...

Embodiment 2

[0044] A process for fermenting and extracting L-isoleucine, comprising the steps of:

[0045] 1) Corynebacterium glutamicum ATCC14309 seed solution (1×10 8 CFU / mL) was transferred to the fermentation medium according to the inoculum size of 8% for cultivation, the temperature was 30°C, the ventilation rate was 3L / min, and the cultivation time was 60 hours to obtain the L-isoleucine fermentation liquid; Sodium hydroxide aqueous solution is used to control the pH of the fermentation broth to be 7.0, and the residual sugar is controlled at not less than 1.2% by feeding a glucose solution with a concentration of 100g / L;

[0046] Fermentation medium components are: glucose 10%, corn steep liquor 3%, ammonium sulfate 3%, calcium carbonate 1%, dipotassium hydrogen phosphate 0.5%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.2%, ferrous sulfate 0.001% , VB1 0.0001%, pH 7.0;

[0047] Use a ceramic membrane to filter the L-isoleucine fermentation liquid to separate the filt...

Embodiment 3

[0053] The impact of various factors of the present invention on the yield of L-isoleucine products is set up as a control group: wherein, control group 1: after the fermentation is completed, directly enter the extraction step, without ultrasonic treatment, tourmaline powder treatment and dialysis culture treatment, and the rest Same as Example 1; Control group 2: After the fermentation is completed, only dialysis culture treatment is carried out, without ultrasonic treatment and tourmaline powder treatment, and the rest are the same as in Example 1; Control group 3: After the fermentation is completed, ultrasonic treatment and dialysis culture treatment are carried out. No tourmaline powder treatment, the rest are the same as in Example 1; control group 4: After the fermentation is completed, tourmaline powder treatment and dialysis culture treatment are carried out, no ultrasonic treatment is used, the rest is the same as in Example 1; L-isoleucine in each group is detected ...

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Abstract

The invention relates to a process for fermenting and extracting L-isoleucine, which comprises the following steps: step 1) fermenting and preparing L-isoleucine, step 2) decolorizing, step 3) concentrating and coarse crystallization, step 4) removing Miscellaneous and crystallization, high-purity boutique L-isoleucine is obtained through the above steps. The process of the invention has high fermentation efficiency, simple operation, high degree of automation, abandons traditional ion exchange steps, and is energy-saving and environment-friendly.

Description

technical field [0001] The invention belongs to the extraction process of L-isoleucine in the biological fermentation industry, and specifically provides a process for extracting L-isoleucine by fermentation. Background technique [0002] L-isoleucine, also known as "isoleucine". The scientific name is "α-amino-β-methylpentanoic acid" aliphatic neutral amino acid. The basic building blocks of protein. L-isoleucine, L-leucine and L-valine are collectively referred to as branched-chain amino acids. As a kind of essential amino acids for human body, they are mainly used in compound amino acid infusion, three-branched-chain amino acid infusion, amino acid oral liquid, etc. It is used in the treatment of symptoms such as liver disease, hepatic coma, and weakness. It is a necessity for amino acid raw materials. It can also be used in food, health products and feed additives, and has a good market prospect. [0003] The applicant's previous patent CN105274179A discloses a proces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/06C07C227/40C07C229/08C12R1/15
CPCC07B2200/07C07C227/40C12P13/06C07C229/08Y02P20/10
Inventor 李学朋刘西龙包鑫张宗华刘洁宋房房刘元涛沈伟伟
Owner XINJIANG FUFENG BIOTECH
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