Application of spirulina powder as binder
A technology of spirulina algae powder and binder, which is applied in the direction of alginic acid binder, adhesive, non-polymer binder additives, etc., to achieve the effects of resisting cancer cell proliferation, improving immune function, and reducing production energy consumption
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Embodiment 1
[0019] Embodiment 1, high-sugar spirulina algae cultivation
[0020] Spirulina cells are cultured in a nutrient-rich medium (Zarrouk) prepared with deionized water containing: NaHCO per liter of water 3 16.8g, K 2 HPO 4 0.5g, NaNO 3 2.5g, K 2 SO 4 1g, NaCl 1g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.04g, FeSO 4 ·7H 2 O 0.01g, H 3 BO 3 2.86mg, MnCl 2 4H 2 O 1.86mg, ZnSO 4 ·7H 2 O0.22mg, Na 2 MoO 4 2H 2 O 0.39mg, CuSO 4 ·5H 2 O 0.08mg. Initial inoculation density OD 560 0.5, culture temperature 30±2℃, continuous single-row light, light intensity 200μmol m –2 the s –1 , into the air flow rate of 0.4vvm. The polysaccharide accumulation process was carried out in a 6L plastic square basin with a water depth of 8cm.
[0021] Grow to OD 560 3 cells were centrifuged at 4000rpm for 5min, the supernatant was discarded, and the algal mud was used without NaNO 3 Zarrouk medium (Zarrouk-N) was washed once and then resuspended in Zarrouk-N to adjust the cell den...
Embodiment 2
[0026] Embodiment 2, large-scale high-sugar spirulina production
[0027] Large-scale cultivation of Spirulina using N limitation in an outdoor racetrack pond for enhanced biomass and polysaccharide yields.
[0028] The process of algae cultivation and polysaccharide accumulation was carried out in an outdoor 1-acre track pool with a water depth of 30 cm and a water flow velocity of 20 cm / s.
[0029] 1) Cultivation of algal species
[0030] Spirulina cells are cultured in nutrient-rich natural mother liquor alkali medium (MYJ), and the culture medium is prepared with underground fresh water, and each liter of water contains: 6g of mother liquor alkali (containing NaHCO 3 about 3.4g), NaNO 3 1g, KCl0.5g, H 3 PO 4 0.12g, FeSO 4 ·7H 2 O 0.01g, MgSO 4 ·7H 2 O 0.01 g. Initial inoculation density OD 560 0.3, the culture temperature is 32±5°C, the natural conditions are illuminated, and the maximum light intensity on the surface of the algae liquid is 3000 μmol m –2 the s ...
Embodiment 3
[0037] Embodiment 3, high-sugar spirulina algae powder tabletting
[0038] According to the traditional spirulina tabletting parameters, high-sugar spirulina powder is directly used to obtain stable algae tablets. Compared with the tablet obtained by conventionally adding 1-2% carboxymethylcellulose sodium, there is no significant difference in its appearance, friability, and disintegration time limit.
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