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Application of phospholipid rich in DHA (Docosahexaenoic Acid) to product for improving acute kidney injury

A technology for acute kidney injury and phospholipids, which is applied in the extraction and preparation of phospholipids, the application in the improvement or treatment of acute kidney injury products, and the prevention field, can solve problems such as no relevant research reports, and achieves low cost, simple steps, low pollution effect

Inactive Publication Date: 2018-07-24
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Recent studies have found that w-3 polyunsaturated fatty acids (n3 polyunsaturated fatty acids), such as docosahexaenoic acid (DHA), can protect against various diseases, including atherosclerosis, diabetes, cancer, inflammation, however The effect of fish oil rich in polyunsaturated fatty acids on acute kidney injury has not been reported
At present, the DHA products on the market are mainly ethyl ester type and glyceride type. Compared with glyceride type and ethyl ester type unsaturated fatty acids, the unsaturated fatty acids in phospholipids not only have better oxidation stability, but also have faster digestion and absorption speed. , plus phospholipids themselves have activities such as improving fat metabolism and enhancing immunity. So far, there has been no relevant research report on the efficacy of DHA-rich phospholipids in improving acute kidney injury. Therefore, DHA-rich phospholipids may be used as a A Novel Lipid Health Product for Improving Acute Kidney Injury

Method used

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  • Application of phospholipid rich in DHA (Docosahexaenoic Acid) to product for improving acute kidney injury
  • Application of phospholipid rich in DHA (Docosahexaenoic Acid) to product for improving acute kidney injury
  • Application of phospholipid rich in DHA (Docosahexaenoic Acid) to product for improving acute kidney injury

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Experimental program
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Effect test

Embodiment 1

[0024] Embodiment 1: the preparation that is rich in DHA phospholipid

[0025] (1) Commercially available cuttlefish eggs were vacuum freeze-dried and pulverized, then ground into powder (200 mesh).

[0026] (2) Take dry squid roe powder, add ethanol, homogenize with a homogenizer for 50 minutes, filter and spin dry the ethanol extract by vacuum rotary evaporation, the obtained extract is ethanol extract, dissolve the ethanol extract Put in chloroform-methanol mixture, then transfer to a separatory funnel, add sodium chloride solution, shake well and let stand overnight, collect the lower chloroform layer, concentrate under reduced pressure to obtain the crude squid roe lipid extract;

[0027] (3) After activating the silica gel column, equilibrate the silica gel column with chloroform; then dissolve the lipid crude extract of squid roe obtained in step (2) with chloroform, slowly pour it into the silica gel column, and then use chloroform, chloroform - eluting with methanol ...

Embodiment 2

[0030] Embodiment 2: animal experiments

[0031] 1. Materials and Methods

[0032] (1) Animal feeding and behavioral analysis

[0033] As experimental animals, Balb / c mice, male, weighing 20-22 g, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. The humidity in the animal room was 65±15%, the room temperature was 23±2°C, light and dark alternated 12h:12h, and the mice could eat and drink freely. After one week of adaptive feeding, the mice were divided into 8 groups: normal control group, model control group, PC, DHA, DHA-PC, DHA-PS, DHA-PE and DHA-PI groups, with 19 mice in each group , respectively fed the corresponding feed. The feed was modified with reference to the AIN-93G rodent feed formula, the food intake was recorded and counted, and the weight change was recorded every other day, and the feeding was continued for 1 week. Except for the normal group, the other five groups were intraperitoneally injected with vancomycin hydrochlo...

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Abstract

The invention discloses application of phospholipid rich in DHA (Docosahexaenoic Acid) to a product for improving the acute kidney injury. According to the invention, DHA phospholipid is prepared by using sleeve-fish roes as raw materials, separation and conversion are further carried out, and purity of the prepared phospholipid is all 90% or above. A preparation method of sleeve-fish phosphatidylcholine extract is low in cost and simple in step, uses a small volume of organic solvent, and generates little organic solvent pollution to the environment. Animal experiments prove that when a mousesuffering from the acute kidney injury is only supplemented and intervened with 200 to 400mg / kg DHA-rich phosphatidylcholine, life time of the mouse suffering from the acute kidney injury is obviously prolonged and the kidney tissue injury degree of the mouse is reduced, which proves that the phospholipid rich in DHA can be applied to the product for improving the acute kidney injury.

Description

technical field [0001] The invention belongs to the technical field of active substance screening, and in particular relates to a method for extracting and preparing DHA-rich phospholipids and its application in products for preventing, improving or treating acute kidney injury. Background technique [0002] Acute kidney injury (acute kidney injury, AKI) is a type of severe disease mainly caused by renal ischemia or nephrotoxic drugs. Manifested as acute tubular necrosis (acute tubular necrosis, ATN). Existing studies have shown that the main pathogenesis of AKI includes mitochondrial damage, inflammatory response, oxidative stress response, renal tubular epithelial cell necrosis and apoptosis, etc. Since AKI has a very complicated pathogenesis and there is still a lack of effective treatment methods, the research on the specific pathogenesis and prevention of AKI has become one of the urgent problems in the field of nephrology. [0003] At present, there is no effective t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/685A61K31/683A61K31/202A61P13/12A23L33/115
CPCA61K31/685A23L33/115A23V2002/00A61K31/202A61K31/683A61K2300/00A23V2200/30A23V2250/1846A23V2250/1848A23V2250/1852A23V2250/185A23V2250/1868
Inventor 王玉明史豪豪薛长湖张恬恬薛勇李兆杰徐杰常耀光王静凤唐庆娟
Owner OCEAN UNIV OF CHINA
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