Complex microbial agent for degrading straw, preparation method of complex microbial agent and method for degrading straw

A technology of compound bacterial agent and straw, which is applied in the preparation of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of inconvenient transport home and incineration on the spot, insufficient utilization, etc., and achieve rapid and full utilization. Degradation, the effect of reducing the cost of use

Active Publication Date: 2018-08-17
友美(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] After the crops are harvested in rural areas, there is a huge amount of st

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing a composite bacterial agent for degrading straw, comprising the following steps:

[0030] Take 30g of soil samples rich in rice stalks from the farmland around Wuhu City, Anhui Province, add 5g of rice stalk segments and 250ml deionized water in shake flasks for enrichment for 15 days, then take the soil solution and dilute it to 10 -1 to 10 -6 For different gradients, take 0.1ml and spread them on the straw powder medium and Congo red-cellulose medium and culture them at 37°C±2°C for 2-3 days, and select the single colony that grows well on the straw powder medium and has a large transparent circle Streak culture was repeated on the plate until pure strains were obtained. XJA1 and XJH1 were identified as Lysinibacillus macroides and Paenibacillus barcinonensis respectively by molecular identification.

[0031] Lysinibacillus macroides and Paenibacillus barcinonensis were inoculated into beef extract-peptone liquid medium at a ratio of 1:1, at a...

Embodiment 2

[0035] A method for degrading stalks, comprising the steps of:

[0036] 1) Prepare the liquid fermentation enzyme production medium, inoculate the compound bacterial agent on the liquid fermentation enzyme production medium according to the ratio of 3%, and cultivate it on a shaker at 180 ± 10 r / min for 1 day under the condition of 37°C ± 2°C to obtain the fermentation liquid;

[0037] The preparation method of the liquid fermentation enzyme production medium is as follows:

[0038] Take KH 2 PO 4 1.00g, CaCl 2 0.10g, MgSO 4 ·7H 2 O 0.30g, NaCl 0.10g, FeCl 3 0.01g and (NH 4 ) 2 SO 4 3.50g, add water to make up to 1000ml.

[0039] 2) Prepare 100ml of the same liquid fermentation enzyme production medium as in step 1), add 2.5g of straw (the straw is dried to constant weight, and the length is 2-3cm) and 3% of the liquid fermentation enzyme production medium prepared in step 1) For the fermentation broth, the straw is degraded at a temperature of 37°C±2°C at a sha...

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Abstract

The invention discloses a complex microbial agent for degrading straw, a preparation method of the complex microbial agent and a method for degrading the straw. The complex microbial agent for degrading the straw is obtained through mixed culture of lysinibacillus macroides and paenibacillus barcinonensis. Pectinase, cellulase, manganese peroxidase, lignin peroxidase and the like, which are relatively high in activity, can be generated; on the basis of enzyme system components which are on a relatively high amount and cause a coordinated effect on the straw, such components as pectic substances, cellulose, hemicellulose, lignin and the like can be degraded, so that rapid and full degradation of the straw is achieved.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a composite bacterial agent for degrading straws, a preparation method thereof, and a method for degrading straws. Background technique [0002] After the crops are harvested in rural areas, there is a huge amount of straw. Except some of them are used for feed, most of them are used as firewood. In fact, crop straw is a treasure. It contains a lot of organic matter, nitrogen, phosphorus, potassium and trace elements needed by crops. It is a very good fertilizer resource when used well. [0003] Straw biodegradation technology is to use the compatibility of microbial strains that can degrade straw and improve the soil, transform crop straw into carbon dioxide, organic matter, and trace elements that can be directly used by a new generation of plants, release heat, and achieve effective nutrition cycle, so that Crop melons, fruits, and vegetables change quali...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/00C05F17/00C12R1/07
CPCC05F11/00C05F17/00C12N1/20C12N1/205C12R2001/07C05F11/08Y02W30/40
Inventor 刘爱民刘宇陈亚玲陈立志李晓磊程鹏
Owner 友美(北京)生物科技有限公司
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