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A preparation method of Prussian blue nanoparticles loaded with polypeptide

A Prussian blue and nanoparticle technology, applied in the field of preparation of Prussian blue nanoparticles, can solve the problems of difficult to achieve precise treatment, lack of active targeting function, failure to accurately reach the lesion site, etc., and achieve good biological safety, Good biocompatibility and easy operation

Active Publication Date: 2021-05-28
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the synthesized Prussian blue nanoparticles lack the function of active targeting, making them unable to accurately reach the lesion in practical applications, making it difficult to achieve precise treatment. Therefore, the preparation of a Prussian blue nanoparticle that can be actively targeted Especially important for medical diagnosis

Method used

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  • A preparation method of Prussian blue nanoparticles loaded with polypeptide
  • A preparation method of Prussian blue nanoparticles loaded with polypeptide
  • A preparation method of Prussian blue nanoparticles loaded with polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1): Dissolve 0.02 mmol of potassium ferrocyanide and 0.5 mmol of citric acid in 20 ml of deionized water, and mix thoroughly to obtain a clear solution A; dissolve 0.02 mmol of ferric chloride and 0.5 mmol of citric acid In 20 ml of deionized water, mix thoroughly to obtain a clear solution B;

[0032] 2): Add solution A dropwise to solution B, react at 60°C to obtain aqueous solution C containing Prussian blue nanoparticles, then add 0.0006 mmoles of polyetherimide to solution C, react at 60°C for 0.5 h, the reaction system is cooled to room temperature, centrifuged and washed to obtain a precipitate D containing aminated Prussian blue nanoparticles;

[0033] 3): Dissolve the precipitate D containing aminated Prussian blue nanoparticles and 0.1 mmol of maleic anhydride in 40 ml of dimethyl sulfoxide, react at room temperature for 3 days, and centrifuge and wash to obtain carboxylated Prussian blue nanoparticles. Precipitation of particles E;

[0034] 4): Precipitate ...

Embodiment 2

[0038] 1): Dissolve 0.056 mmol of sodium ferricyanide and 1.12 mmol of citric acid in 20 milliliters of deionized water, and mix well to obtain a clear solution A; dissolve 0.056 mmol of ferrous chloride and 1.12 mmol of citric acid In 20 ml of deionized water, mix thoroughly to obtain a clear solution B;

[0039] 2): Add solution A dropwise to solution B, react at 60°C to obtain aqueous solution C containing Prussian blue nanoparticles, then add 0.001 mmol of polyetherimide to solution C, and react at 60°C After 0.5h, the reaction system was cooled to room temperature, centrifuged and washed to obtain a precipitate D containing aminated Prussian blue nanoparticles;

[0040] 3): Precipitation D containing aminated Prussian blue nanoparticles and 0.25 mmoles of succinic anhydride were dissolved in 40 ml of acetone, reacted at room temperature for 2 days, centrifuged and washed to obtain precipitate E containing carboxylated Prussian blue nanoparticles ;

[0041]4): Precipitat...

Embodiment 3

[0046] 1): Dissolve 0.04 mmol of ammonium ferrocyanide and 0.6 mmol of malic acid in 20 ml of deionized water, mix thoroughly to obtain clear solution A; dissolve 0.04 mmol of ferric chloride and 0.6 mmol of malic acid In 20 ml of deionized water, mix thoroughly to obtain a clear solution B;

[0047] 2): Add solution A dropwise to solution B, and react at 60°C to obtain aqueous solution C containing Prussian blue nanoparticles, then add 0.0008 mmol of polypropylene amine hydrochloride to solution C, and heat at 60°C The reaction was carried out for 1 hour, and the reaction system was cooled to room temperature, and centrifuged and washed to obtain a precipitate D containing aminated Prussian blue nanoparticles;

[0048] 3): Dissolve the precipitate D containing aminated Prussian blue nanoparticles and 0.16 mmol of maleic anhydride in 40 ml of dimethyl sulfoxide, react at room temperature for 3 days, and centrifuge and wash to obtain carboxylated Prussian blue nanoparticles. P...

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Abstract

The invention relates to a preparation method of Prussian blue nanoparticles capable of loading polypeptides. The preparation method includes the synthesis of Prussian blue nanoparticles and their surface functional modification, using the specific combination of maleimide groups modified on the surface of nanoparticles and sulfhydryl groups to load biologically active targeting polypeptides. The preparation method of the present invention is simple to operate, green and environment-friendly, and the raw materials are readily available. The prepared nanoparticles have the advantages of good biocompatibility and high biosafety, and can actively target lesion areas to realize accurate diagnosis of diseases. The particle size of the Prussian blue nanoparticles prepared by the invention is controllable, has good dispersibility in phosphate buffer solution (pH=7.4), and is beneficial for application in living bodies.

Description

technical field [0001] The invention relates to a nanometer material applicable to the field of biomedicine, in particular to a method for preparing polypeptide-loaded Prussian blue nanoparticles. Background technique [0002] Magnetic resonance imaging (Magnetic Resonance Imaging, MRI) is a non-invasive medical imaging diagnostic method. It can image deep tissues by detecting the distribution of hydrogen ions under a pulsed magnetic field. It has the ability to observe axial and sagittal planes simultaneously. , which has a unique advantage in reflecting the weak difference of lesions among tissues, and has no radiation damage to the human body. The MRI contrast agents currently studied mainly include two categories: one is T, which brightens local tissues. 1 Contrast agents, such as trivalent gadolinium ions (Gd 3+ ) of paramagnetic chelates; the other is T that darkens local tissues 2 Contrast agents, such as iron oxide nanoparticle contrast agents with paramagnetic pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/18A61K49/14A61K49/08
CPCA61K49/085A61K49/14A61K49/1866
Inventor 常津刘雅娟窦妍
Owner TIANJIN UNIV
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