Probiotic grape seed extract mask and preparation method thereof
A grape seed and probiotic technology, applied in the field of skin care products, can solve problems such as greater skin damage and less than ideal effects, and achieve the effect of delicate and smooth skin, beneficial to skin health, and free radical scavenging.
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[0034] The preparation method of above-mentioned grape seed fermented liquid is:
[0035] a. Separate the grape seeds from the grape marc after wine making, add 0.05mol / L sodium hydroxide solution according to the material-to-liquid ratio of 1:10, soak in a water bath at 80°C for 30 minutes, repeat soaking three times, and then rinse with water Clean, dry and crush through a 40-mesh sieve to obtain grape seed powder;
[0036] b. Transfer the Bacillus licheniformis preserved on the slant to the plate, and after culturing for 24 hours, insert a single colony into the seed culture solution with a liquid volume of 20 / 100ml, and culture it with shaking at 32°C and 150r / min for 14-16 hours. Add the seed liquid with 10% inoculum amount to the enzyme-producing medium with a liquid content of 60 / 250ml, and culture at 34°C and 150r / min for 8 hours with shaking to obtain a mixed solution of Bacillus licheniformis and alkaline protease;
[0037] c. Add 50% mixed solution according to the...
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