Probiotic grape seed extract mask and preparation method thereof
A grape seed and probiotic technology, applied in the field of skin care products, can solve problems such as greater skin damage and less than ideal effects, and achieve the effect of delicate and smooth skin, beneficial to skin health, and free radical scavenging.
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[0034] The preparation method of the above-mentioned grape seed fermentation liquid is:
[0035] a. Separate the grape seeds from the grape pomace after making wine, add 0.05mol / L sodium hydroxide solution at a material-to-liquid ratio of 1:10, soak in a water bath at 80°C for 30 minutes, repeat the soaking three times, and then rinse with water Clean, dry and crush through a 40-mesh sieve to obtain grape seed powder;
[0036] b. Transfer the Bacillus licheniformis preserved on the slant to the plate. After culturing for 24 hours, insert a single colony into a seed culture medium with a volume of 20 / 100ml, and culture at 32°C, 150r / min, shaking for 14-16h, Insert the seed solution with 10% inoculum into an enzyme production medium with a filling amount of 60 / 250ml, and culture at 34°C, 150r / min, shaking for 8h to obtain a mixture of Bacillus licheniformis and alkaline protease;
[0037] c. Add 50% mixture according to the material-liquid ratio of 1:12, incubate at 34°C, 150r / min, sh...
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