Synephrine composition for treating gastrointestinal dysfunction or irritable bowel syndrome and application thereof
A technology for irritable bowel syndrome and gastrointestinal dysfunction, applied in the field of natural pharmaceutical compositions, to achieve targeted production quality control, enhanced pharmacological effects and clinical efficacy, and stable and controllable drug quality
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experiment example 1
[0075] Effect of Composition on Visceral Hypersensitivity in Irritable Bowel Syndrome Model Rats
[0076] 1. Experimental animals and medicines are provided through legal channels. Pinaverium Bromide Tablets 50mg / tablet (Abbott Products SAS) for experiments; Synephrine (98%), Glycyrrhizinic Acid (95%), Naringin (98%), Hesperidin (98%), Islicorice for experiments Glycoside (98%), liquiritin (98%) and neohesperidin (98%) were provided by Nantong Feiyu Biotechnology Company. The relevant extracts used in the experiment were prepared by ourselves, synephrine extract (content greater than or equal to 10%), glycyrrhizic acid (content greater than or equal to 20%), naringin (content greater than or equal to 20%), orange peel Glycoside (content greater than or equal to 20%), neohesperidin extract (content greater than or equal to 20%), liquiritin (content greater than or equal to 10%), isoliquiritin (content greater than or equal to 5%). The decoction pieces of traditional Chinese m...
experiment example 2
[0126] Effects of the composition on 5-HT signaling pathway and TRPV1 in visceral hypersensitive rats.
[0127] 1. Experimental animals, medicines, modeling, and test product preparation: the same as in Experimental Example 1.
[0128] 2. Experimental method
[0129] Rat colon tissue sample collection: 2 hours after the last gavage, the rats were executed by decapitation, and 3 segments of the colon 3 cm above the anus were taken, each segment 1-2 cm, washed with normal saline and PBS buffer solution (pH 7.4) , blotted dry with filter paper, accurately weighed, quickly frozen with liquid nitrogen, and stored at -80°C for later use.
[0130]5-HT, 5-HT3R, 5-HT4R, TPH1, and SERT protein expression levels in rat colon were measured by enzyme-linked immunosorbent assay (ELISA). The rat colon tissue pieces were washed with PBS buffer, and the tissue was homogenized by adding appropriate saline. slurry, and centrifuged to obtain the supernatant. According to the instructions of th...
experiment example 3
[0154] The adjustment of experimental example 3 to the gastrointestinal dysfunction rat caused by reserpine
[0155] 1. Animals and reagents are the same as in Experimental Example 1.
[0156] 2. Animal grouping and dosage: (1) Normal group was injected intraperitoneally with equal volume of normal saline, and at the same time fed with equal volume of distilled water; (2) Model group: intraperitoneally injected with reserpine 0.5 mg / kg, and simultaneously fed with equal volume of distilled water (3) Positive group: intraperitoneal injection of reserpine 0.5mg / kg, and at the same time, 50 mg / kg of Suliqenone was administered; (4) sample group: intraperitoneal injection of reserpine 0.5mg / kg, and simultaneously, the test samples were administered (with experimental example one). Each group was administered continuously for two weeks. The feces and signs of the rats were observed at any time, and the body weight and food intake of the rats were weighed every day. The following...
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