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Novel fermented Lactobacillus and applications of novel fermented Lactobacillus in fields of feeds and sewage treatment

A fermenting Lactobacillus, a new type of technology, applied in the field of microorganisms, can solve the problems of easy inactivation, poor salt tolerance, etc., and achieve the effect of reducing odorous substances, increasing intestinal activity, and stabilizing the effect

Active Publication Date: 2018-09-18
浙江亲水园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems that existing lactobacilli have poor acid resistance, salt resistance, and easy inactivation, the present invention provides a new type of fermenting lactobacillus and its application in the field of feed and sewage treatment. The fermenting lactobacillus LBF128 of the present invention has Acid resistance, salt resistance, high temperature resistance, strain LBF128 or its mutants that can reproduce under anaerobic, aerobic and facultative conditions, acid resistance means that it can survive for more than 2 hours in an environment with a pH as low as 2.0. Salt performance refers to the ability to survive and function in environments with up to 6% salt

Method used

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  • Novel fermented Lactobacillus and applications of novel fermented Lactobacillus in fields of feeds and sewage treatment
  • Novel fermented Lactobacillus and applications of novel fermented Lactobacillus in fields of feeds and sewage treatment
  • Novel fermented Lactobacillus and applications of novel fermented Lactobacillus in fields of feeds and sewage treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Acid resistance test

[0058] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, the pH values ​​of the medium were adjusted to 3.0 and 2.0 with dilute hydrochloric acid, respectively. After cultivating for 1 hour and 2 hours, respectively detect the content of LBF128 lactic acid bacteria in the petri dish, the results are as follows:

[0059] Experimental results

[0060] The acid resistance test result of table 1 lactobacillus

[0061]

[0062] The above experiments prove that LBF128 lactic acid bacteria have strong acid resistance and can survive for more than 2 hours in an environment of pH=2.0. Under acidic conditions of pH=3.0, LBF128 lactic acid bacteria can not only survive but also maintain reproductive capacity. Therefore, it is considered that it can basically pass through the strongly acidic stomach enviro...

Embodiment 2

[0064] Salt tolerance test

[0065] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then adjust the saline environment containing salinity 6% (mass concentration) with salt, cultivate the LBF128 lactic acid bacteria content in the culture dish after 2 hours and 4 hours respectively, the results are as follows:

[0066] Salt tolerance experiment result of table 2 lactobacillus

[0067]

[0068] The above experiment proves that LBF128 lactic acid bacteria has a strong salt resistance ability, and can survive for more than 4 hours in an environment with a salt concentration as high as 6%, indicating that the LBF128 lactic acid bacteria of the present invention can survive and work in high-salinity water such as seawater.

Embodiment 3

[0070] High temperature test

[0071] Experimental process: LBF128 lactic acid bacteria were cultivated with diluted agar medium in a petri dish, and the content of LBF128 lactic acid bacteria in the petri dish was adjusted to 10 7 / mL, then place the petri dish in an environment of 60°C and 80°C for cultivation, and detect the content of LBF128 lactic acid bacteria in the petri dish after cultivating for 1 hour and 2 hours, the results are as follows:

[0072] Salt tolerance experiment result of table 3 lactobacillus

[0073]

[0074] The above experiments prove that LBF128 lactic acid bacteria have a strong ability to resist high temperature, and can still survive and work in a high temperature environment of 80°C.

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Abstract

The invention discloses novel fermented Lactobacillus and applications of the novel fermented Lactobacillus in the fields of feeds and sewage treatment, and belongs to the field of microorganisms. According to the present invention, the novel fermented Lactobacillus is Lactobacillus Fermentum strain LBF128, has characteristics of acid resistance, salt tolerance and high-temperature resistance, canbe propagated under anaerobic, aerobic and facultative conditions, and can survive for more than 2 h under the pH value of 2.0, wherein more than 70% of the live bacteria can reach the intestine after the novel fermented Lactobacillus passes through the stomach due to the breeding under the anaerobic, aerobic and facultative conditions; the novel fermented Lactobacillus can be used as the additive for livestock and poultry feeds, and can further be used for the biological deodorization of manure, wherein the effect is stable, and the pollutants (COD of more than 25000) in the original manure)can be degraded to below 1500; and the novel fermented Lactobacillus can be used for the treatment of domestic sewage, acidic high-salt sewage and river basin sewage, has advantages of good acid resistance, good salt resistance, high survival rate and significant treatment effect, and can survive and work in the environment with the salt content of up to 6%.

Description

technical field [0001] The invention belongs to the field of microorganisms, and more specifically relates to a novel fermenting lactobacillus and its application in the fields of feed and sewage treatment. Background technique [0002] Lactobacillus refers to bacteria that can ferment sugar to produce lactic acid. It is a group of microorganisms that live in the body and benefit the health of the host. Its role in maintaining human health and regulating immune function has been widely recognized. Lactobacillus is a genus of Lactobacillus family, named for the large amount of lactic acid produced by fermenting sugar. Widely distributed in nature, it is one of the important physiological flora in the intestinal tract of animals and humans. The vast majority of bacteria in this group are non-toxic and harmless to animals and humans, and they are responsible for important physiological functions in animals. Among lactic acid bacteria, Lactobacillus is the largest genus, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18C02F3/34C12R1/225
CPCA23K10/18C02F3/34C12N1/20C12N1/205C12R2001/225
Inventor 丁庆
Owner 浙江亲水园生物科技有限公司
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