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Layered magnetic fluorescence nanometer assembly capable of triggering nuclear explosion of cancer cells

A fluorescent nanotechnology, cancer cell nucleus technology, applied in nanotechnology, nanotechnology, luminescent materials, etc., can solve problems such as the inability to ideally and accurately provide the morphological information of the chemotherapy mechanism of targeted agents, limited binding stability, and limited optical stability. , to achieve the effect of good application prospect and simple preparation process

Inactive Publication Date: 2018-09-28
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing phenotypic characterization technology uses transmission electron microscopy for cell steady-state analysis. Most of the optical reagents used for dynamic imaging tracking are fluorescein or biological dyes. The binding stability and optical stability of these materials and drugs or preparations are limited, which is not ideal. , Accurately provide morphological information of the chemotherapy mechanism of targeted agents

Method used

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  • Layered magnetic fluorescence nanometer assembly capable of triggering nuclear explosion of cancer cells
  • Layered magnetic fluorescence nanometer assembly capable of triggering nuclear explosion of cancer cells
  • Layered magnetic fluorescence nanometer assembly capable of triggering nuclear explosion of cancer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: DMF@QD layered magnetic fluorescent nano-assembly with a mass fraction of 1:3 was prepared, and the physical and chemical properties of the sample were characterized.

[0039] (1) One-pot synthesis of ZnHgSe quantum dots in aqueous phase:

[0040] a. Weigh 0.5131g of Zn(NO 3 ) 2 ·6H 2 O solid is put into 1000mL reactor, add 900mL water to dissolve until clarification, in N 2 Oxygen removal for 30 minutes under circulation, room temperature and 300rpm magnetic stirring conditions; molar ratio of Zn 2+ / Hg 2+ =1:0.05 ratio, take 863μL of Hg(NO 3 ) 2 4H 2 O solution (0.01mol·L -1 ) was added to the above solution to disperse evenly. Molebi Zn 2+ / MPA=1:1.8 ratio Add 22μL of MPA to the above mixed solution, react under in situ conditions for 50min, then add 2.0mol·L dropwise -1 NaOH solution, adjust the end point pH of the slurry to 8.5 to make Zn 2+ -Hg 2+ - MPA precursor;

[0041] b. Molar ratio of Se / NaBH 4 =1:2 Weigh 0.017g Se powder and 0.016g...

Embodiment 2

[0046] Example 2: DMF@QD layered magnetic fluorescent nano-assembly with a mass fraction of 1:2 was prepared; a concentration of 1.4 mg / μL was used to act on human gastric cancer MGC-803 cells for live cell imaging.

[0047] (1) Preparation of ZnHgSe quantum dots: Weigh 5.1313g of Zn(NO 3 ) 2 ·6H 2 O solid is put into 1000mL reactor, add 900mL water to dissolve until clarification, in N 2 Deoxygenation for 30min under protection, room temperature and 300rpm magnetic stirring conditions, add 8.6mL concentration 0.01mol L -1 Hg(NO 3 ) 2 4H 2 O solution, stirred and mixed in situ for 30min, then added dropwise 0.22mL of MPA, reacted in situ for 45min; then, added dropwise 2.0mol·L -1 NaOH solution, control the end point pH of the slurry to 9.0, made Zn 2+ -Hg 2+ -MPA precursor.

[0048] Weigh 0.17g Se powder and 0.16g NaBH 4 Solid phase, put into 100mL reactor, add 10mL double distilled water, 2 Under the conditions of protection, 50°C constant temperature and 300rpm m...

Embodiment 3

[0052] Example 3: Preparation of DMF@QD layered magnetic fluorescent nano-assembly with a mass fraction of 1:1; acting on human gastric cancer MGC-803 cells at a concentration of 1.2 mg / μL, live cell imaging tracking; evaluating in vitro killing by MTT method capacity of cancer cells.

[0053] (1) Preparation of ZnHgSe quantum dots: conditions and process (same as Example 2 (1))

[0054] a. Weigh 0.5131g of Zn(NO 3 ) 2 ·6H 2 O solid is put into 1000mL reactor, add 900mL water to dissolve until clarification, in N 2 Oxygen removal for 30 minutes under circulation, room temperature and 300rpm magnetic stirring conditions; molar ratio of Zn 2+ / Hg 2+ =1:0.05 ratio, take 862.5μL of Hg(NO 3 ) 2 4H 2 O solution (0.01mol·L -1 ) into the above solution to disperse evenly. Molebi Zn 2+ / MPA=1:1.8 ratio Add 21.5μL of MPA to the above mixed solution, react under in situ conditions for 50min, then add 2.0mol·L dropwise -1 NaOH solution, adjust the end point pH of the slurry to...

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Abstract

The invention relates to a layered magnetic fluorescence nanometer assembly capable of triggering nuclear explosion of cancer cells. The layered magnetic fluorescence nanometer assembly is characterized by being assembled with a dextran-magnetic layered double hydroxides-fluorouracil magnetic slow-released drug delivery system serving as a therapeutic drug, a water-soluble near-infrared ZnHgSe quantum dot serving as a fluorescent tracer through an electrostatic composite technology. The nanometer assembly can start an oncosis path of the cancer cells, and trigger cell nucleus explosion and cytoclasis; with the assistance of a cell imaging technology, the morphological change of the oncosis process of the cells can be disclosed. The preparing technology is simple and efficient, the producthas a function integrating diagnosis and treating, and has special meanings and good application prospects in the aspect of disclosing an oncosis mechanism of the cells and clearing away tumor lesions.

Description

technical field [0001] The present invention relates to a chemotherapeutic preparation, specifically, a drug compounded by fluorouracil magnetic slow-release drug (dextran-magnetic layered double hydroxide-fluorouracil system, DMF) and fluorescent quantum dots (QD), which can trigger cancer cell nuclear explosion and cell disintegration. Layered magnetic fluorescent nanoassemblies. Background technique [0002] Physical targeting preparations are prepared with viral carriers, organic cationic compounds, recombinant proteins, and inorganic nanoparticles as carriers. The highest targeting targets that can be achieved are organelles such as mitochondria, which usually cause cancer cells to die in the form of apoptosis; Limited by the development technology of new vectors, the preparations targeting the nucleus are rarely reported at home and abroad. Magnetic layered double hydroxides (MLDH) are structurally integrated with Fe(OH) x The paramagnetism and LDH's drug slow and co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/513A61K49/00A61P35/00C09K11/88B82Y20/00B82Y40/00
Inventor 苟国敬金学琴王锐裴琴玉姚惠琴张玉梅左玲霞
Owner NINGXIA MEDICAL UNIV
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